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EC number: 276-337-4 | CAS number: 72102-30-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- FROM 15 OCT 2018 TO 30 NOV 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- The temperature for the study ranged from 20.4 to 22.8 °C and therefore higher than stated in the Guideline and fluctuated by more than 2°C. The deviation was only short due to the relocation of the data logger.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Fatty acids, vegetable-oil, Me esters, sulfurized
- EC Number:
- 276-337-4
- EC Name:
- Fatty acids, vegetable-oil, Me esters, sulfurized
- Cas Number:
- 72102-30-8
- IUPAC Name:
- Fatty Acids, vegetable-oil, Me-Esters, sulfurized
- Test material form:
- liquid
- Details on test material:
- - Density: 0.95 g/mL
- Batch no.: 71012767
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The water-accommodated fraction (WAF) was prepared for the test. This was done by mixing of 100.1 mg/L resp. 105.4 μL/L test item (based on a density of 0.95 g/mL stated in the SDS) with the corresponding amount of dilution water and stirring moderately for 24 hours. The lower phase was used unfiltered as test solution. The same preparation procedure was used for the medium renewal. For this solution the WAF was stirred for 23 hours 30 minutes.
- Controls: At the start and at the end of the test, the content of the test item in the test solutions was determined using GC/MSD.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): not stated
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Berlin
- Age at study initiation (mean and range, SD): between 0 and 24 hours
- Stage and instar at study initiation: newborn daphnia
- Method of breeding: in-house breeding
- Source: LAUS GmbH
- Feeding during test: not stated
ACCLIMATION
- Acclimation period: not stated
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES, INCLUDING CULTURING CONDITIONS:
The husbandry is performed similar to the method described in the OECD guideline, following SOP 115 002 01 („Zucht und Hälterung von Daphnia magna STRAUS“), version 12 from 02. Feb. 2015.
Vessels preserving glasses, nominal volume 2 L
Medium M4-Medium (recipe of ELENDT)
Food green algae (Desmodesmus subspicatus)
Medium renewal twice a week
Photo period 16/8 hours, using neon tubes
Temperature 20 ± 2 °C
21 hours before the start of the test, the adult animals were separated from the young. 40 minutes before test start, the adults were caught with the help of a glass tube, and the newborn daphnia, aged between 0 and 21 hours, were sieved from the medium and immediately placed into a beaker containing dilution water. After the settling-in period, animals which showed no apparent damage were used for the test.
Switching from M4-medium (husbandry) to Dilution water (test) has been shown not to cause any detrimental effects for test daphnia.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Hardness in mmol/L: 2.502
- Test temperature:
- 20.4 – 22.8 °C
- pH:
- pH-values
Nominal Concentration 0 h 24 h 24 h 48 h
in mg/L old new
Blank control 7.7 7.8 7.8 7.8
100 7.7 7.9 7.9 7.9 - Dissolved oxygen:
- O2-Concentration in mg/L
Nominal Concentration 0 h 24 h 24 h 48 h
in mg/L old new
Blank control 8.9 9.0 9.2 8.5
100 8.9 8.8 8.9 8.6 - Conductivity:
- not stated
- Nominal and measured concentrations:
- 100 mg/L nominal concentration
- Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers
- Type: open
- Material, size, headspace, fill volume: glass, nominal volume 100 mL, tall shape
- Volume of solution: 50 ± 5 mL
- Aeration: no
- Renewal rate of test solution: renewal was performed after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionised water with an enrichment of certain minerals (following SOP 11800201) was used in the test.
- Total organic carbon: not stated
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: not stated
- Light intensity: not stated
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Daphnia are considered immobilised if they perform no movements or are only able to move their antennae when the beaker is gently agitated. Observation times 24 and 48 hours. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: no behavioural abnormalities reported
- Observations on body length and weight: not reported
- Other biological observations: not reported
Other adverse effects control: no adverse effects of control reported
- Immobilisation of control: 1/20
- Abnormal responses: no abnormal responses reported
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not reported
- Effect concentrations exceeding solubility of substance in test medium: yes - Results with reference substance (positive control):
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study (201714R201). The 24h-EC50i value was determined as 1.9 mg/L.
The value was within the requested range for the 24h-EC50 between 0.6 - 2.1 mg/L of potassium dichromate K2Cr2O7 as specified in the OECD guideline. Since the fits showed sufficient statistical correspondence of the data with the dose-response-equation, this reference study can be considered valid.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study was performed as a limit test at 100 mg/L. For the test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours in a semi-static test system. After 24 and 48 hours, the immobilised Daphnia were counted. Because the test item is not stable under test conditions the study was performed under semi-static conditions.
The treatment showed no toxicity/mortality. One of the animals was immobilised in the blank control. Therefore no statistical evaluation was performed.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable.
At the start and at the end of the test, the content of the test item in the test solutions was determined using GC/MSD.
At the beginning of the test the test item was still detectable in small quantities. No test item was detectable after 24 h and 48 h incubation. Since the test item was stirred and therefore could not be removed from the solution by filtration, test item was definitely present in the solution.
Therefore, the results are related to the nominal values.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable.
No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met.
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