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EC number: 204-310-9 | CAS number: 119-27-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study completed October 9, 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.2500 (Acute Dermal Irritation)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,4-dinitroanisole
- EC Number:
- 204-310-9
- EC Name:
- 2,4-dinitroanisole
- Cas Number:
- 119-27-7
- Molecular formula:
- C7H6N2O5
- IUPAC Name:
- 2,4-dinitroanisole
1
- Specific details on test material used for the study:
- The test material was stored at room temperature. The Sponsor was responsible for any necessary evaluations related to identity, strengh, purity, composition, stability and method of synthesis of the test material according to 40 CFR 160.105 and 40 CFR 792.105.
The Sponsor was responsible for maintaining a retention sample of the test article.
The test article was administered as reveived from the Sponsor and dispensed fresh on the day of dosing.
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- Plastic ear tags displaying unique identification numbers were used to individually identify the animals.
Animals were housed individually in suspended stainless steel cages.
Animals room temperature and relative humidity ranges were 66-70°F and 43-49%.
PMI Certified Rabbit Chow was provided ad libitum.
Municipal tap water treated by reverse osmosis was available ad libitum throughout the study.
Upon receipt, animals were acclimated to the laboratory conditions for a minimun of 5 days.
Animals were approximately 11 weeks old and weighted 2,3-2,5 kg at study initiation.
Light timers were set to maintain a 12-hour light-12-hour dark cycle and roon ventilation was set to produce 10-15 air changes-hour.
Test system
- Type of coverage:
- semiocclusive
- Preparation of test site:
- clipped
- Remarks:
- Care was taken to avoid abrading the skin during the clipping procedure.
- Vehicle:
- water
- Amount / concentration applied:
- 0,5g applied
Concentration 100% - Duration of treatment / exposure:
- 4h exposure period
- Observation period:
- 1 hour after patch removal
24, 48 and 72h after patch application - Number of animals:
- 3
- Details on study design:
- The test article was moistened with the appropriate amount of deionized water to form a paste and then administered under the gauze patch. Tha gauze patch was held in contact with the skin at the cut edges with a nonirritating tape.
Removal and ingestion of the test article was prevented by placing an elastic wrap over the trunk and test area (semi-occlusive binding).
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- 24 h
- Score:
- ca. 1
- Reversibility:
- fully reversible within: 48h
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- other: 1 h
- Score:
- ca. 1
- Reversibility:
- fully reversible within: 48h
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- 48 h
- Score:
- ca. 0
- Reversibility:
- fully reversible
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- 72 h
- Score:
- ca. 0
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- other: 1h
- Score:
- ca. 0
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- ca. 0
- Irritation parameter:
- erythema score
- Basis:
- animal #3
- Time point:
- other: 1h
- Score:
- ca. 0
- Irritation parameter:
- erythema score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- ca. 0
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- other: 1h
- Score:
- ca. 0
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- ca. 0
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- other: 1h
- Score:
- ca. 0
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- ca. 0
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- other: 1h
- Score:
- ca. 0
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- ca. 0
- Irritant / corrosive response data:
- The responses were scored according to the Macroscopic Dermal Grading System which is based on Draize, which is the same as the system used in the 404 OECD guideline.
- Other effects:
- Any unusual observations and/or mortality were recorded.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Not classified. Score of 0.25.
- Executive summary:
In a primary skin irritation study, performed according the OPPTS Guideline 870.2500, in GLP compliance, 3 New Zealand rabbits were used to assess irritant and/or corrosive potential effect of 2,4 -Dinitroanisole. Each of 3 rabbits received a 0,5g dose of the test article in a single dermal application. The dose was held in contact with the skin under a semi-occlusive binder.
Exposure to DNAN produced a very slight erythema on 2/3 test sites at the 1/hour scoring interval. The dermal irritation resolved completely in the affected animals by the 48 -hour scoring interval.
The Draize's score is 0.25.
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