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EC number: - | CAS number: -
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Short term toxicity Daphnia magna: EC50 > 100 % v/v saturated solution, NOEC = 100 % v/v saturated solution, OECD 202, EU Method C2, Goodband & Mullee (2009).
Key value for chemical safety assessment
Additional information
In the key study Goodband & Mullee (2009), the short term toxicity of the test material to Daphnia magna was determined in a GLP study performed according to the standardised guidelines OECD 202 and EU Test Method C2, in an acute immobilisation test. The study was assigned a reliability score of 1 in line with the principles for assessing data quality as defined by Klimisch (1997).
Pre-study solubility work showed that whilst the test material was readily soluble/dispersable in deionised reverse osmosis water, the test material formed a precipitate. Following a media preparation trial it was considered appropriate to prepare the test media by the use of a saturated solution using a stirring period of 24 hours followed by filtration (0.2µm, 2 litres discarded to pre-condition the filter) to remove any undissolved test material prior to exposure of the test organisms. Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to be less than the limit of quantitation (LOQ) of the analytical method. This does not infer that no test material was in solution but that the dissolved concentration (i.e. bioavailable to the test organisms) was below the limit of quantitation which was assessed down to 0.50 mg/L. The concentration of the test material could not be determined in the test media. Therefore the results are based on concentration as % v/v saturated solution.
Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to a saturated solution of the test material for 48 hours at a temperature of 22 °C under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 24 and 48 hours. A positive control conducted using potassium dichromate, results of which were in the normal range for this reference material.
Under the conditions of the test, no toxic effects were observed at saturation of the test material. Therefore the 48 hours EC50 was determined to be > 100 % v/v saturated solution and correspondingly the NOEC was 100 % v/v saturated solution.
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