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EC number: 218-259-5 | CAS number: 2095-06-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1 February 2013 - 01 February 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was performed according with OECD Guideline No. 209 and in accordance with GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- (2010)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- (2008)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- (2007)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline
- EC Number:
- 275-662-9
- EC Name:
- m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline
- Cas Number:
- 71604-74-5
- Molecular formula:
- C15H19NO4
- IUPAC Name:
- 3-(oxiran-2-ylmethoxy)-N,N-bis(oxiran-2-ylmethyl)aniline
- Test material form:
- liquid: viscous
- Details on test material:
- Description: Clear slightly yellow very viscous liquid
Test substance storage: In refrigerator (2-8°C) in the dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- - Method: The test substance was not completely soluble in test medium at the loading rates initially prepared. Therefore, 1-Litre test bottles were filled with 200 ml of test substance mixtures in Milli-RO water (tap water purified by reverse osmosis) with initial loading rates of 2.5 times the final loading rate. These mixtures were stirred in closed dark brown bottles for 10 minutes. Subsequently, 16 ml synthetic medium, 250 ml sludge and Milli-RO water up to 500 ml were added resulting in the required loading rates. Optimal contact between the test substance and test organisms was ensured applying continuous aeration and stirring
- Controls: a blank-control, an abiotic control and the highest loading rate with a nitrification inhibitor.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: Municipal sewage treatment plant: 'Waterschap Aa en Maas', Heeswijk-Dinther, The Netherlands, receiving predominantly domestic sewage.
- Preparation of inoculum for exposure: The sludge was coarsely sieved (1 mm), washed and diluted with ISO-medium. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.0 g/L of sludge). The pH was 7.1 on the day of testing. The batch of sludge was used one day after collection; therefore 50 ml of synthetic medium was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
- Performance of the test: The synthetic medium (16 ml) and an appropriate amount of the test substance stock were mixed and made up to 250 ml with Milli-RO water in a 1 litre bottle. The pH was determined. Thereafter 250 ml activated sludge was added.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- - After the 3-hour contact time, the oxygen consumption was recorded for a period of 10-11 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer.
The pH and temperature were determined in the remaining part of the reaction mixture. This procedure was repeated for all test/reference substance concentrations/loading rates and controls.
Test conditions
- Test temperature:
- 18 to 20°C
- pH:
- - Before addition of sludge was between 7.4 and 7.5.
- After the 3 hour exposure period the pH was between 7.4 and 8.1. - Dissolved oxygen:
- - At the start of the test the dissolved oxygen concentration was above 60-70% saturation (60% of air saturation is > 5 mg/L at 20°C)
- The respiration rate (R) from each vessel, in mg O2/l.h was calculated or interpolated from the linear part of the respiration curve, which was generally between 2 and 7 mg O2/l.
- Controls: 42 mg O2/l h (mean value)
T1 (a loading rate of 10 mg/l): 41 mg O2/l h
T2 (a loading rate of 100 mg/l): 46 mg O2/l h
T3 (a loading rate of 1000 mg/l): 30 mg O2/l h (mean value) - Nominal and measured concentrations:
- - Nominal: Blank-control and loading rates of 10, 100 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: All-glass bottles
- Type: open
- Material, size, headspace, fill volume: All-glass, 1 L capacity, filled with 500 ml of test solution
- Aeration: Yes. The aeration was adjusted in such a way that the dissolved oxygen concentration at the start was above 60-70% saturation (60% of air saturation is > 5 mg/L at 20°C) and to maintain the sludge flocs in suspension.
- No. of vessels per concentration (replicates): 3 replicates for the 1000 mg/L test concentration and 1 replicate for the other test concentrations
- No. of vessels per control (replicates): Blank-control (6 replicates), nitrification control and abiotic control
- Concentration of suspended solids in test solution: 1.5 g/L of sludge
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ion-exchange cartridges (Milli-Q)
- Intervals of water quality measurement
pH: At the beginning and at the end of the test period.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): After the 3-hour contact time the oxygen consumption was recorded for a period of 10-11 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: x10 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: An average of 28% inhibition was observed at 1000 mg/L
- Details on results:
- - The combined limit/range-finding test showed 2%, no inhibition and an average of 28% inhibition of the respiration rate at a loading rate of 10, 100 and 1000 mg/l, respectively. The inhibition observed at a loading rate of 1000 mg/l was statistically significant (Two Sample t-Test). The EC50 was above the highest loading rate tested (1000 mg/l).
- There was no oxygen uptake from abiotic processes. The results at 1000 mg/l with a nitrification inhibitor showed 52% heterotrophic inhibition of the respiration rate. - Results with reference substance (positive control):
- - The EC50 was 8.9 mg 3,5-Dichlorophenol/L
- Reported statistics and error estimates:
- - ECx
For m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline no EC50 could be calculated because the test substance proved to be non-toxic (EC50 > a loading rate of 1000 mg/l).
- NOEC estimation
In this present test m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline was slightly toxic to waste water (activated sludge) bacteria at all loading rates tested. Therefore, a NOEC could not be determined in the combined limit/range-finding test (Two sample-t-Test α=0.05, Toxstat Release 3.5, 1996, D.D. Gulley, A.M. Boelter, H.L. Bergman). As discussed with the sponsor no further testing was performed to determine the NOEC.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- - Under the conditions of this present test m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline was slightly toxic to waste water (activated sludge) bacteria at or below a loading rate of 1000 mg/l. As discussed with the sponsor no further testing was performed to determine the NOEC.
- The EC50 exceeded a loading rate of 1000 mg/l. - Executive summary:
The influence of m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline on the respiration rate of activated sludge was investigated after a contact time of 3 hours.
The study procedures described in this report were based on the OECD guideline No. 209, 2010. In addition, the procedures were designed to meet the test methods of the Council Regulation (EC) No. 440/2008 of 30 May 2008, Publication No. L142, Part C11 and ISO Standard 8192 (2007).
The batch of m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline tested was a clear slightly yellow very viscous liquid and consisted of 100% Mono constituent substance. No correction was made for the purity/composition of the test substance. The test substance was not completely soluble in test medium at the loading rates initially prepared. Therefore, 1-Litre test bottles were filled with 200 ml of test substance mixtures in Milli-RO water with initial loading rates of 2.5 times the final loading rate. These mixtures were stirred in closed dark brown bottles for 10 minutes. Subsequently, synthetic medium, sludge and Milli-RO water were added resulting in the required loading rates. Optimal contact between the test substance and test medium was ensured applying continuous aeration and stirring during the 3-hour exposure period. Thereafter, oxygen consumption was recorded for 10-11 minutes.
In a combined limit/range-finding test loading rates of 10, 100 and 1000 mg/l were tested. The highest loading rate was tested in triplicate, lower concentrations consisted of one replicate. In addition, blank-controls and a nitrification control of the sludge were included. Furthermore, an abiotic control and the highest concentration with a nitrification inhibitor were tested.
The combined limit/range-finding test showed 2%, no inhibition and an average of 28% inhibition of the respiration rate at a loading rate of 10, 100 and 1000 mg/l, respectively. The inhibition observed at a loading rate of 1000 mg/l was statistically significant (Two Sample t-Test : α=0.05). The EC50 was above the highest loading rate tested (1000 mg/l).
There was no oxygen uptake from abiotic processes. The results at 1000 mg/l with a nitrification inhibitor showed 52% heterotrophic inhibition of the respiration rate.
The batch of activated sludge was checked for sensitivity by testing the reference substance 3,5-dichlorophenol, which showed normal sensitivity.
The study met the acceptability criteria prescribed by the protocol and was considered valid.
In conclusion, m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline was slightly toxic to waste water (activated sludge) bacteria at or below a loading rate of 1000 mg/l. As discussed with the sponsor no further testing was performed to determine the NOEC.
The EC50 exceeded a loading rate of 1000 mg/l.
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