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EC number: 294-304-2 | CAS number: 91697-43-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 12 - 15 Sep 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP-Guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Qualifier:
- according to guideline
- Guideline:
- other: VCI concept for the acute aquatic testing of hardly soluble substances from 31st Jan 1994
- GLP compliance:
- yes
Test material
- Reference substance name:
- Isopropyl myristate
- EC Number:
- 203-751-4
- EC Name:
- Isopropyl myristate
- Cas Number:
- 110-27-0
- Molecular formula:
- C17H34O2
- IUPAC Name:
- isopropyl myristate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method 1: 100 mg/L nominal: Direct weighing-in of TS in Aqua bidest, shaking for 24 h
- Method 2: 100 mg/L filtered test solution: Direct weighing-in of 111 TS in 1 L Aqua bidest, stirring over a period of 24 h; filtration through glass fibre filter where unsoluble residues are removed, preconditioning of the glass fibre filter with 1 mol NaOH
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Source (laboratory, culture collection): laboratory culture, source Institute of Plant physiology, University of Göttingen, Germany
- Method of cultivation: 10000 cells/mL used as initial cell count for renewed cultures
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21.9 °C
- pH:
- Test begin: 7.9
Test end: 9.1-9.4 (control); 9.4 (test substance, method 1), 7.8 (test substance, method 2) - Nominal and measured concentrations:
- 100 mg/L (nominal) as emulsion (method 1) and as filtered test solution (method 2)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flask
- Material, size, headspace, fill volume: 100 mL test solution
- Initial cells density: 10000 cells/mL
- Control end cells density: 790000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used: according to DIN 38412 part 9, mineral salt medium
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Photoperiod: 24 hours, permanent
- Light intensity and quality: 300 Lux
- other: shaking at 120 rounds per minute
EFFECT PARAMETERS MEASURED: growth
- Determination of cell concentrations: counting chamber (Thoma chamber)
- Determination of cell number at 24, 48 and 72 h
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- < 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: method 1
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.05
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: filtered test solution
- Basis for effect:
- growth rate
- Remarks on result:
- other: method 2
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
Method 1: turbidity was observed in the test solution at all observation times. Method 2: no turbidity of the test solution (filtered).
The biomass was inhibited to 86.8% (area under the curve) and the growth rate was inhibited to 68.3% at 100 mg/L with method 1; the effects are discussed to be caused by the physical effect caused by undissolved chemical substance rather than a systemic toxic effect
- Effect concentrations exceeding solubility of substance in test medium: The limit solubility of the TS is approximately 0.05 mg/L.
Any other information on results incl. tables
Cells/mL counted with Thoma chambers for the different test solutions:
Concentration of TS |
Incubation time (in h) |
||
24 |
48 |
72 |
|
Control |
63000 |
290000 |
790000 |
100 mg/L emulsion (method 1) |
27000 |
73000 |
40000 |
100 mg/L filtrate (method 2) |
73000 |
290000 |
820000 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.