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EC number: 210-764-9 | CAS number: 623-00-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-01-15 - 1997-02-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-bromobenzonitrile
- EC Number:
- 210-764-9
- EC Name:
- 4-bromobenzonitrile
- Cas Number:
- 623-00-7
- Molecular formula:
- C7H4BrN
- IUPAC Name:
- 4-bromobenzonitrile
- Reference substance name:
- 2-bromobenzonitrile
- EC Number:
- 218-045-1
- EC Name:
- 2-bromobenzonitrile
- Cas Number:
- 2042-37-7
- Molecular formula:
- C7H4BrN
- IUPAC Name:
- 2-bromobenzonitrile
- Reference substance name:
- 3-bromobenzonitrile
- EC Number:
- 230-127-9
- EC Name:
- 3-bromobenzonitrile
- Cas Number:
- 6952-59-6
- Molecular formula:
- C7H4BrN
- IUPAC Name:
- 3-bromobenzonitrile
- Reference substance name:
- Benzonitrile
- EC Number:
- 202-855-7
- EC Name:
- Benzonitrile
- Cas Number:
- 100-47-0
- Molecular formula:
- C7H5N
- IUPAC Name:
- benzonitrile
- Reference substance name:
- Water
- EC Number:
- 231-791-2
- EC Name:
- Water
- Cas Number:
- 7732-18-5
- Molecular formula:
- H2O
- IUPAC Name:
- Water
- Test material form:
- solid
Constituent 1
impurity 1
impurity 2
impurity 3
impurity 4
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic (adaptation not specified)
- Details on inoculum:
- The source of the test organisms was activated sludge freshly obtained from a municipal sewage treatment plant: 'Watershap de Maaskant', s-Hertogenbosch, the Netherlands.
The sludge was kept under continuous eration until further treatment. The concentration of suspended solids was 4.3 g/L in the concentrated sludge. Before use, the sludge was allowed to setle for at least 30 min and the liquid was decanted for use as inoculum at the amount of 10 mL/L of mineral medium. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- ca. 26 mg/L
- Based on:
- test mat.
- Initial conc.:
- ca. 12 mg/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
TEST CONDITIONS
- Composition of medium:
Stock solutions:
a) 08.50 g KH2PO4
21.75 g K2HPO4
67.20 g Na2HPO4 * 12 H2O
00.50 g NH4Cl
dissolved in 1 L purified deionized water, pH 7.4 +/- 0.2
b) 22.50 g MgSO4 * 7 H2O in 1 L purified deionized water
c) 36.40 g CaCl2 * 2 H2O in 1 L purified deionized water
d) 00.25 g FeCl3 * 6 H2O in 1 L purified deionized water
Mineral medium:
a) 10 mL
b) - d) 1 mL
filled up to 1 L with purified deionized water
- Additional substrate: Sodium acetate
- Solubilising agent (type and concentration if used):
- Test temperature: 20 - 23 °C
- pH: 7.3 - 7.5
- pH adjusted: yes, mineral medium
- CEC (meq/100 g):
- Aeration of dilution water: yes, CO2 free air
- Suspended solids concentration: 4.3 g/L in concentrated sludge
- Continuous darkness: yes, brown colored glass bottles
- Other:
TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Mixture of O2 (21 %) and N2 (79 %), led through a solution of Ba(OH)2 (0.0125 M) to trap small amounts of CO2.
- Method used to create anaerobic conditions: %
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: 3 CO2 absorbers (bottles filled with 100 ml 0.0125 M Ba(OH)2) in series
- Other:
SAMPLING
- Sampling frequency: every 2nd or 3rd day in the first 10 days, at least every 5th day until 28th day
- Sampling method: CO2 absorber nearest to test bottle was removed for titration, a new absorber was placed at the end of the series.
- Sterility check if applicable:
- Sample storage before analysis:
- Other:
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes, Sodium acetate + test item
- Other:
STATISTICAL METHODS:
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- < 10
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- the percentage degradation of the reference substance reached the level for ready biodegradability (60 %) by 14 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- 4-Brombenzonitrile is not ready biodegradable under the conditions of the modified Sturm test presently performed.
- Executive summary:
4 -Brombenzonitrile was tested for its biodegradability in the carbon dioxide (CO2) evolution test (modified Sturm test) at ca. 52 mg per 2 L, corresponding to ca. 12 mg TOC / L.
The study procedure was based on ECC Directive 92769, C.4 -C, December 1992 and OECD guideline No. 301 B July 17, 1992.
The Theoretical CO2 production (ThCO2) of 4 -Bromobenzonitrile was calculated to be 1.692 CO2/mg.
The relative degradation values calculated from the measurementes perfomed during the test period revealed no significant degradation of 4 -Bromobenzinitrile (significant: >10 %).
In the toxicity control 4 -Bromobenzonitrile was found to be not inhibitory.
Since all acceptability criteria prescribed in by the protocol were met, this study was considered to be valid.
In conclusion. 4 -Bromobenzonitrile was not ready biodegradable under the conditions of the modified Sturm test presently performed.
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