Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 213-584-9 | CAS number: 989-38-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005-01-18 to 2005-12-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 21 Juli 1997
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Version / remarks:
- 19 May 2000
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- Version / remarks:
- August 1998
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- BASF Aktiengesellschaft, Experimental Toxicology and Ecology, 67056 Ludwigshafen, Germany
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthylium chloride
- EC Number:
- 213-584-9
- EC Name:
- 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthylium chloride
- Cas Number:
- 989-38-8
- Molecular formula:
- C28H31N2O3.Cl
- IUPAC Name:
- 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthylium chloride
- Test material form:
- solid
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver S9 fraction
- Test concentrations with justification for top dose:
- 15.625 µg - 5000 µg/plate (STP: Standard plate test)
0.3 µg - 125 µg/plate (PIT: Preincubation test; Salmonella strains)
7.8125 - 125 µg (PIT; Preincubation test; E. coli WP2uvrA) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Due to the Iimited solubility of the test substance in water, DMSO was selected as the vehicle, which had been demonstrated to be suitable in bacterial reverse mutation tests and for which historical control data are available.
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: +S-9 mix; 2-aminoanthracene (2-AA): TA 1535, TA 100, TA 1537, TA 98, E. coli WP2 uvrA;
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: -S-9 mix: N-methyl-N'-nitro-N-nitrosoguanidine (MNNG): TA 1535, TA 100; 4-nitro-o-phenylendiamine (NOPD): TA 98; 9-aminoacridine (AAC): TA 1537; 4-nitroquinoline-N-oxide (4-NQO): E. coli WP2 uvrA
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation); preincubation
Standard platetest
Test tubes containing 2-mL portions of soft agar (overlay agar), which consists of 100 mL agar (0.8% agar + 0.6% NaCl) and 10 mL amino acid solution (minimal amino acid solution
for the determination of mutants: 0.5 mM histidine + 0.5 mM biotin) are kept in a water bath at about 42 - 45 °C, and the remaining components are added in the following order:
0.1 mL test solution or vehicle
0.1 mL fresh bacterial culture
0.5 mL S-9 mix (in tests with metabolic activation)
or
0.5 mL phosphate buffer (in tests without metabolic activation)
After mixing, the samples are poured onto Vogel-Bonner agar plates (minimal glucose agar plates) within approx. 30 seconds.
Composition of the minimal glucose agar:
980 mL purified water
20 mL Vogel-Bonner E medium
15 g Difco bacto agar
20 g D-glucose, monohydrate.
After incubation at 37 °C for 48 - 72 hours in the dark, the bacterial colonies (his~ revertants) are counted.
Escherichia coli
Test tubes containing 2-mL portions of soft agar (overlay agar), which consists of 100 mL agar (0.8% agar + 0.6% NaCI) and 10 ml amino acid solution (minimal amino acid solution
for the determination of mutants: 0.5 mM tryptophan) are kept in a water bath at about 42 - 45 °C, and the remaining components are added in the following order:
0.1 mL test solution or vehicle
0.1 mL fresh bacterial culture
0.5 mL S-9 mix (in tests with metabolic activation)
or
* 0.5 mL phosphate buffer (in tests without metabolic activation)
After mixing, the samples are poured onto minimal agar plates within approx. 30 seconds. Composition of the minimal agar:
The composition of the minimal agar (SAl selective agar) is based on the description of Green, M.H.L. and Muriel, W.J. (5), with the exception of solution E (tryptophan solution),
which has previously been added to the soft agar:
300 mL solution B (agar)
100 mL solution A (sahne solution)
8 mL solution C (glucose solution)
10 mL solution D (casein solution)
After incubation at 370 °C for 48 - 72 hours in the dark, the bacterial colonies (trp~ revertants) are counted.
Preincubation Test:
0.1 mL test solution or vehicle, 0.1 mL bacterial suspension and 0.5 mL S-9 mix or phosphate buffer are incubated at 37 °C for the duration of about 20 minutes using a shaker. Subsequently, 2 mL of soft agar is added and, after mixing, the samples are poured onto the agar plates within approx. 30 seconds. After incubation at 37 °C for 48 - 72 hours in the dark, the bacterial colonies are counted.
Titer determination
In the standard plate test, 0.1 mL of the overnight cultures is diluted to 10^6 in each case.
Test tubes containing 2-mL portions of soft agar containing maximal amino acid solution (5 mM tryptophan or 5 mM histidine + 0.5 mM biotin) are kept in a water bath at about 42 - 450 °C, and the remaining components are added in the following order:
0.1 mL vehicle (without and with test substance)
0.1 mL fresh bacterial culture (dilution: 10-6)
0.5 mL S-9 mix
In the preincubation test, 0.1 mL of the overnight cultures is diluted to 10^-6 in each case. 0.1 mL vehicle (with and without test substance), 0.1 mL bacterial suspension and 0.5 mL S-9 mix are incubated at 370 °C for about 20 minutes using a shaker. Subsequently, 2 mL of soft agar containing maximal amino acid solution for titer determination (5 mM tryptophan or 5 mM histidine + 0.5 mM biotin) is added. After mixing, the samples are poured onto the agar plates within approx. 30 seconds. After incubation at 370 °C for 48 - 72 hours in the dark, the bacterial colonies are counted.
NUMBER OF REPLICATIONS: 3 - Evaluation criteria:
- ACCEPTANCE CRITERIA:
Generally, the experiment is considered valid if the following criteria are met:
- The number of revertant colonies in the negative controls was within the normal range of the historical control data for each tester strain.
- The sterility controls revealed no indication of bacterial contamination.
- The positive control articles both with and without S-9 mix induced a significant increase in the number of revertant colonies within the range of the historical control data or above.
- The titer of viable bacteria was ≥ 10^8/ mL.
ASSESSMENT CRITERIA:
The test chemical is considered positive in this assay if the following criteria are met:
- A dose-related and reproducible increase in the number of revertant colonies, i.e. about doubling of the spontaneous mutation rate in at least one tester strain either without S-9 mix or after adding a metabolizing system.
A test substance is generally considered nonmutagenic in this test if:
- The number of revertants for all tester strains were within the historical negative control range under all experimental conditions in two experiments carried out independently of each other.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 500 µg - 5000 µg/Plate (+/-S9 mix)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 100 µg - 5000 µg/Plate (+/-S9 mix)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 100 µg - 5000 µg/Plate (+/-S9 mix)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 500 µg - 5000 µg/Plate (+/-S9 mix)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 500 µg - 5000 µg/Plate (-S9 mix); 2500 µg - 5000 µg/Plate (+S9 mix)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 30 - 125 µg/plate (+/-S9 mix)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 30 - 125 µg/plate (+/-S9 mix)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 30 - 125 µg/plate (+/-S9 mix)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 30 - 125 µg/plate (+/-S9 mix)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 125 µg/plate (+/-S9 mix)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: No Precipitation of the test substance was found
ADDITIONAL INFORMATION ON CYTOTOXICITY:
- Toxicity: A strong bacteriotoxic effect was observed under all test conditions. - Remarks on result:
- other: Standard plate test
Any other information on results incl. tables
Standard Plate Test (10.02.2005)
Dose/ Plate |
Mean number of revertant colonies/ plate (± SD) with different strains of Salmonella typhimurium and one Escherichia coli strain |
||||
TA 1535 |
TA 100 |
TA 1537 |
TA 98 |
E. coli WP2 uvrA |
|
-S9 mix |
|||||
DMSO |
16 ± 1 |
102 ± 11 |
13 ± 3 |
33 ± 7 |
45 ± 9 |
20 µg |
23 ± 5 |
95 ± 12 |
14 ± 6 |
24 ± 1 |
47 ± 7 |
100 µg |
16 ± 3 |
34 ± 6 B |
5 ± 1 B |
27 ± 4 |
35 ± 2 |
500 µg |
0B |
0B |
0B |
6 ± 1 B |
26 ± 1 B |
2500 µg |
0B |
0B |
0B |
0B |
18 ± 2 B |
5000 µg |
0B |
0B |
0B |
0B |
2 ± 2 B |
MNNG 5.0 µg |
1218 ± 133 |
1379 ± 121 |
- |
- |
- |
AAC |
- |
- |
980 ± 65 |
- |
- |
NOPD |
- |
- |
- |
1009 ± 95 |
- |
4-NQO |
- |
- |
- |
- |
1065 ± 106 |
+S9 mix |
|||||
DMSO |
21 ± 4 |
104 ± 13 |
13 ± 1 |
39 ± 2 |
44 ± 3 |
20 µg |
23 ± 1 |
113 ± 5 |
13 ± 4 |
38 ± 2 |
39 ± 3 |
100 µg |
20 ± 3 |
67 ± 16 B |
9 ± 4 B |
33 ± 4 |
35 ± 8 |
500 µg |
0B |
0B |
0B |
6 ± 3 B |
18 ± 3 |
2500 µg |
0B |
0B |
0B |
0B |
10 ± 4 B |
5000 µg |
0B |
0B |
0B |
0B |
4 ± 1 B |
2-AA 2.5 µg |
182 ± 20 |
1564 ± 294 |
107 ± 6 |
1544 ± 192 |
306 ± 16 |
Standard Plate Test (18.02.2005)
Dose/ Plate |
Mean number of revertant colonies/ plate (± SD) with different strains of Salmonella typhimurium and one Escherichia coli strain |
||||
TA 1535 |
TA 100 |
TA 1537 |
TA 98 |
E. coli WP2 uvrA |
|
-S9 mix |
|||||
DMSO |
20 ± 3 |
116 ± 9 |
10 ± 3 |
28 ± 2 |
32 ± 4 |
15.625 µg |
19 ± 2 |
134 ± 3 |
9 ± 2 |
26 ± 2 |
28 ± 4 |
31.25 µg |
19 ± 3 |
120 ± 5 |
13 ± 3 |
28 ± 4 |
28 ± 7 |
62.5 µg |
18 ± 1 |
89 ± 4 |
8 ± 2 |
27 ± 3 |
29 ± 4 |
125 µg |
15 ± 2 |
48 ± 6 |
7 ± 1 |
17 ± 3 |
25 ± 4 |
250 µg |
11 ± 3 |
5 ± 3 |
4 ± 2 |
20 ± 3 |
24 ± 4 |
MNNG 5.0 µg |
673 ± 26 |
625 ± 106 |
- |
- |
- |
AAC |
- |
- |
578 ± 57 |
- |
- |
NOPD |
- |
- |
- |
783 ± 30 |
- |
4-NQO |
- |
- |
- |
- |
594 ± 39 |
+S9 mix |
|||||
DMSO |
18 ± 1 |
127 ± 18 |
13 ± 2 |
34 ± 4 |
40 ± 5 |
15.625 µg |
20 ± 3 |
136 ± 7 |
10 ± 2 |
29 ± 5 |
36 ± 5 |
31.25 µg |
19 ± 1 |
129 ± 14 |
10 ± 2 |
28 ± 4 |
38 ± 3 |
62.5 µg |
17 ± 3 |
118 ± 11 |
10 ± 1 |
29 ± 2 |
31 ± 13 |
125 µg |
14 ± 3 |
104 ± 9 |
10 ± 2 |
25 ± 5 |
32 ± 12 |
250 µg |
16 ± 2 |
43 ± 43 |
5 ± 2 |
25 ± 2 |
25 ± 4 |
2-AA 2.5 µg |
177 ± 8 |
1010 ± 129 |
135 ± 10 |
857 ± 25 |
245 ± 27 |
Preincubation Test (22.02.2005)
Dose/ Plate |
Mean number of revertant colonies/ plate (± SD) with different strains of Salmonella typhimurium and one Escherichia coli strain |
||||
TA 1535 |
TA 100 |
TA 1537 |
TA 98 |
E. coli WP2 uvrA |
|
-S9 mix |
|||||
DMSO |
18 ± 2 |
110 ± 3 |
12 ± 3 |
32 ± 3 |
31 ± 2 |
7.8125 µg |
14 ± 1 |
108 ± 6 |
11 ± 1 |
32 ± 6 |
29 ± 3 |
15.625 µg |
15 ± 1 |
103 ± 2 |
8 ± 1 |
22 ± 4 |
31 ± 6 |
31.25 µg |
12 ± 3 B |
69 ± 10 B |
4 ± 1 B |
11 ± 2 B |
28 ± 4 |
62.5 µg |
0B |
11 ± 3 B |
0B |
6 ± 3 B |
24 ± 3 |
125 µg |
0B |
0B |
0B |
0B |
22 ± 7 B |
MNNG 5.0 µg |
744 ± 26 |
832 ± 29 |
- |
- |
|
AAC |
- |
- |
475 ± 24 |
- |
- |
NOPD |
- |
- |
- |
954 ± 18 |
- |
4-NQO |
- |
- |
- |
- |
571 ± 55 |
+S9 mix |
|||||
DMSO |
17 ± 2 |
107 ± 3 |
12 ± 3 |
37 ± 7 |
39 ± 5 |
7.8125 µg |
14 ± 2 |
104 ± 5 |
11 ± 2 |
28 ± 3 |
32 ± 4 |
15.625 µg |
12 ± 2 |
110 ± 6 |
8 ± 1 |
20 ± 4 |
36 ± 5 |
31.25 µg |
10 ± 2 B |
98 ± 3 B |
6 ± 2 B |
17 ± 3 B |
28 ± 7 |
62.5 µg |
6 ± 1 B |
72 ± 3 B |
7 ± 0 B |
13 ± 2 B |
30 ± 2 |
125 µg |
4 ± 2 B |
31 ± 5 B |
4 ± 1 B |
7 ± 2 B |
28 ± 3 B |
2-AA 2.5 µg |
155 ± 17 |
885 ± 55 |
158 ± 18 |
1021 ± 86 |
240 ± 17 |
Preincubation Test (02.03.2005)
Dose/ Plate |
Mean number of revertant colonies/ plate (± SD) with different strains of Salmonella typhimurium and one Escherichia coli strain |
|||
TA 1535 |
TA 100 |
TA 1537 |
TA 98 |
|
-S9 mix |
||||
DMSO |
17 ± 2 |
107 ± 3 |
9 ± 3 |
30 ± 5 |
0.3 µg |
16 ± 3 |
104 ±7 |
8 ± 2 |
28 ± 3 |
1 µg |
15 ± 3 |
111 ± 4 |
10 ± 2 |
30 ± 6 |
3 µg |
14 ± 2 |
100 ± 4 |
8 ± 2 |
30 ± 7 |
10 µg |
12 ± 2 |
87 ± 4 |
6 ± 2 |
22 ± 3 |
30 µg |
9 ± 1 B |
47 ± 20 B |
4 ± 3 B |
9 ± 1 B |
MNNG 5.0 µg |
811 ± 96 |
750 ± 40 |
- |
- |
AAC |
- |
- |
902 ± 58 |
- |
NOPD |
- |
- |
- |
635 ± 53 |
4-NQO |
- |
- |
- |
- |
+S9 mix |
||||
DMSO |
18 ± 2 |
108 ± 10 |
10 ± 1 |
31 ± 2 |
0.3 µg |
15 ± 1 |
104 ± 3 |
8 ± 2 |
28 ± 7 |
1 µg |
15 ± 3 |
107 ± 7 |
11 ± 3 |
29 ± 3 |
3 µg |
17 ± 3 |
103 ± 7 |
7 ± 1 |
27 ± 6 |
10 µg |
11 ± 3 |
100 ± 6 |
7 ± 2 |
21 ± 3 |
30 µg |
11 ± 6 B |
84 ± 11 B |
6 ± 2 B |
10 ± 2 B |
2-AA 2.5 µg |
107 ± 8 |
902 ± 58 |
383 ± 41 |
806 ± 41 |
SD: Standard deviation
B: Reduced background growth
Applicant's summary and conclusion
- Conclusions:
- Under the experimental conditions chosen, the test substance is not mutagenic in the Salmonella typhimurium/Escherichia coli reverse mutation assay.
- Executive summary:
The test substance (in DMSO) was tested in the Salmonella typhimurium reverse mutation assay with four histidine-requiring strains of Salmonella typhimurium (TA 1535, TA 1537, TA 100 and TA 98) and in Escherichia coli reverse mutation assay with a tryptophan-requiring strain of Escherichia coli WP2 uvrA according to OECD guideline 471.
The test substance was tested up to concentrations of 20 µg to 5000 µg/plate in the standard plate test (STP). In the preincubation tests concentrations of 0.3 µg to 125 µg/plate (S. typhimurium) and 7.7128 µg to 125 µg/plate (E. coli) were tested. No test substance precipitation was found. A bacteriotoxic effect (reduced his- or trp- background growth, decrease in the number of his+ or trp+ revertants, reduction in the titer) was observed in the standard plate test depending on the strain and test conditions from about 100 µg to 500 µg/plate onward. In the preincubation assay bacteriotoxicity (reduced his- background growth, decrease in the number of his+ revertants, reduction in the titer) was observed depending on the Salmonella strain and test conditions from about 30 µg/plate onward. Using E. coli WP2 uvrA toxicity was found at doses ≥ 62.5 µg/plate.
An increase in the number of his+ or trp+ revertants was not observed in the standard plate test or in the preincubation test either without S-9 mix or after the addition of a metabolizing system. According to the results of the present study, the test substance is not mutagenic in the Salmonella typhimurium/Escherichia coli reverse mutation assay under the experimental conditions chosen here.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.