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EC number: 915-650-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1 December 2009 to 30 December 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- although minor deviations from guideline are reported (see 'Any other information on materials and methods'), these are not considered to have affected the overall reliability of the test.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- Version 1992
- Deviations:
- yes
- Remarks:
- see 'Any other information on materials and methods incl. tables'.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Version / remarks:
- Version 1992
- Deviations:
- yes
- Remarks:
- see 'Any other information on materials and methods incl. tables'.
- Qualifier:
- according to guideline
- Guideline:
- ISO 10707 Water quality - Evaluation in an aqueous medium of the "ultimate" aerobic biodegradability of organic compounds - Method by analysis of biochemical oxygen demand (closed bottle test)
- Version / remarks:
- Version 1994
- Deviations:
- yes
- Remarks:
- see 'Any other information on materials and methods incl. tables'.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Food and Consumer Product Safety Authority (VWA). Prinses Beatrixlaan 2, 2595 AL Den Haag. Postbus 19506, 2500 CM Den Haag, The Netherlands (d.d. 19 March 2008).
- Specific details on test material used for the study:
- - Solubility in water: 49 mg/L
- Test material storage: At ambient temperature in the dark - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source: Secondary activated sludge (25-11-2009) was obtained from the wastewater treatment plant Nieuwgraaf in Duiven, The Netherlands. This plant is an activated sludge plant treating predominantly domestic wastewater.
- Pre-conditioning: The activated sludge was pre-conditioned to reduce the endogenous respiration rates. To this end, 400 mg Dry Weight (DW)/L of activated sludge was aerated for one week. The sludge was diluted in the BOD bottles. - Duration of test (contact time):
- 28 d
- Initial conc.:
- ca. 2 mg/L
- Based on:
- test mat.
- Initial conc.:
- ca. 5.8 mg/L
- Based on:
- ThOD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The nutrient medium of the Closed Bottle test contained per liter of deionized water: 8.5 mg KH2P04, 21.75 mg K2HP04, 33.3 mg Na2HP04·2H2O, 22.5 mg MgS04·7H2O, 27.5 mg CaCl2, 0.25 mg FeCl3·6H2O. Ammonium chloride was omitted from the medium to prevent nitrification.
- Test concentrations: Administration of myrac aldehyde was accomplished by dosing 0.03 g of myrac aldehyde on 10 g of silica gel in a 50-ml serum flask. Only part of the top layer of the silica gel was brought into contact with the fragrance substance. The serum flask was closed with a screw top with alumiminium foil and the content was mixed vigorously. Subsequently 0.2 g of silica gel dosed with myrac aldehyde was added to the test bottles. The resulting concentration of test substance in the bottles is ~2.0 mg/L. Next the bottles were filled with nutrient medium with inoculum and closed. Each of the prepared solutions was dispensed into the respective group of BOD bottles so that all bottles were completely filled without air bubbles.
- Reference substance: Sodium acetate was added to the bottles at a concentration of 6.7 mg/L (5.36 mg/L based on ThOD). For preparations of reference solutions a stock solution of 1.0 g/L was used.
- Test bottles: The test was performed in 0.30 L BOD (biological oxygen demand) bottles with glass stoppers. Use was made of 10 bottles containing only inoculum, 10 bottles containing inoculum and silica gel, 10 bottles containing inoculum and silica gel dosed with test substance test substance and silica gel, and 6 bottles containing sodium acetate and inoculum.
- Toxicity control: Inhibition of the degradation of a well-degradable compound, e.g. sodium acetate by the test substance in the Closed Bottle test was not determined because possible toxicity of myrac aldehyde to microorganisms degrading acetate is not relevant. Inhibition of the endogenous respiration of the inoculum by the test substance was not detected. Therefore, no inhibition of the biodegradation due to the "high" initial concentration of the test substance is expected.
- Sampling: The zero time bottles were immediately analyzed for dissolved oxygen using an oxygen electrode. The remaining bottles were closed and incubated in the dark. Two duplicate bottles of all series were withdrawn for analyses of the dissolved oxygen concentration at day 7, 14, 21, and 28.
- Temperature: The temperature was measured and recorded with a sensor connected to a data logger. Temperatures were within the prescribed temperature range of 22 to 24°C.
- pH: The pH was measured using a Knick 765 calimatic pH meter (Elektronische Messgerate GmbH, Berlin, Germany). The pH of the media was 7.0 at the start of the test. The pH of the medium at day 28 was 6.9. - Reference substance:
- acetic acid, sodium salt
- Remarks:
- 6.7 mg/L based on test mat., 5.36 mg/L based on ThOD
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 71
- Sampling time:
- 28 d
- Details on results:
- The pass level of 60% was not reached within 10 days upon achieving 10% biodegradation. Myrac aldehyde therefore only fulfilled one criterion for ready biodegradable compounds i.e. a biodegradation percentage in excess of 60 within 28 days. The reason for failing the 10-day window is the composition of myrac aldehyde. Myrac aldehyde is a mixture of two isomers 3-and 4-( 4-methyl-3-penten-1-yl)-3-cyclohexene-1-carbaldehyde. One isomer is present at ~70% and the other at ~30%. The degradation curve is therefore the sum of two growth curves. The 10-day time window criterion was developed on the assumption that a single compound is degraded according to a "standard" growth curve in ready biodegradability tests. The time-window should therefore be ignored as a pass fail criterion for myrac aldehyde. Myrac aldehyde is therefore classified as readily biodegradable only based on the biodegradation percentage of 71 % at day 28.
- Validity criteria fulfilled:
- yes
- Remarks:
- see 'Any other information on materials and methods incl. tables'.
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The substance is readily biodegradable under the conditions of the test.
- Executive summary:
The biodegradation potential of 3-CYCLOHEXENE-1-CARBOXALDEHYDE MULTICONSTITUENT was examined in a study according to OECD TG 301D (Closed Bottle test) and in compliance with GLP criteria.
In this study 2 mg/L test substance (ca. 5.8 mg/L based on ThOD) was inoculated for 28 days under aerobic conditions and in the dark with secondary activated sludge obtained from a municipal wastewater treatment plant treating predominantly domestic wastewater. Two duplicate bottles of all series were withdrawn for analyses of the dissolved oxygen concentration at day 0 and after 7, 14, 21, and 28 exposure and the cumulative percentages biodegradation calculated from the oxygen depletion ratio BOD/ThOD. Minor deviations from the guideline included omitting ammonium chloride from the medium to prevent oxygen consumption due to nitrification and use of secondary activated sludge as inoculum instead of an effluent/extract/mixture. Also, the pH was 7.0 at the start of the test instead of the recommended pH of 7.4 ± 0.2. None of these deviations are however considered to have affected the overall reliability or outcome of the test. All validity criteria were met.
After the 28-day incubation period the substance was biodegraded 71%. The 10-day window criterion was not met. The reason for failing the 10-day window is that the test substance is a mixture of two isomers present at 70% and 30%, respectively. The degradation curve is therefore the sum of two growth curves whereas the 10-day time window criterion was developed on the assumption that a single compound is degraded according to a ‘standard’ growth curve in ready biodegradability tests. The time-window may therefore be ignored as pass/fail criterion and the substance should be classified as readily biodegradable based on the biodegradation percentage of 71% at day 28 alone.
Reference
Table: Dissolved oxvqen concentrations (mg/L) in the closed bottles
Time (days) |
Oxygen concentration (mg/L) |
|||
Ocs |
Ot |
Oc |
Oa |
|
0 |
8.7 |
8.7 |
8.7 |
8.7 |
8.7 |
8.7 |
8.7 |
8.7 |
|
Mean (M) |
8.7 |
8.7 |
8.7 |
8.7 |
7 |
7.8 |
7.5 |
7.8 |
3.6 |
7.9 |
7.6 |
7.8 |
3.6 |
|
Mean (M) |
7.9 |
7.6 |
7.8 |
3.6 |
14 |
7.4 |
4.5 |
7.4 |
2.9 |
7.5 |
4.2 |
7.4 |
2.8 |
|
Mean (M) |
7.5 |
4.4 |
7.4 |
2.9 |
21 |
7.4 |
4.1 |
7.3 |
- |
7.3 |
4.2 |
7.3 |
- |
|
Mean (M) |
7.4 |
4.2 |
7.3 |
- |
28 |
7.4 |
3.3 |
7.3 |
- |
7.4 |
2.9 |
7.3 |
- |
|
Mean (M) |
7.4 |
3.1 |
7.3 |
- |
Ocs: Mineral nutrient solution without test material but with inoculum and silica gel.
Ot: Mineral nutrient solution with test material (ca. 2.0 mg/L), silica gel, and inoculum.
Oc: Mineral nutrient solution with only inoculum.
Oa: Mineral nutrient solution with sodium acetate (ca. 6.7 mg/L) and with inoculum.
Table: Oxygen consumption (mg/L) and the percentages biodegradation of the test substance, myrac aldehyde (BOD/ThOD) and sodium acetate (BOD/ThOD) in the Closed Bottle test.
Time (days) |
Oxyqen consumption (mq/L) |
Biodegradation (%) |
||
Test substance |
Acetate |
Test substance |
Acetate |
|
0 |
0.0 |
0.0 |
0 |
0 |
7 |
0.3 |
4.2 |
5 |
78 |
14 |
3.0 |
4.5 |
52 |
83 |
21 |
3.1 |
- |
53 |
- |
28 |
4.1 |
- |
71 |
- |
Description of key information
The biodegradation potential of 3 -CYCLOHEXENE-1 -CARBOXALDEHYDE MULTICONSTITUENT was examined in a study according to OECD TG 301D (Closed Bottle test) and in compliance with GLP criteria.
In this study 2 mg/L test substance (ca. 5.8 mg/L based on ThOD) was inoculated for 28 days under aerobic conditions and in the dark with secondary activated sludge obtained from a municipal wastewater treatment plant treating predominantly domestic wastewater. Two duplicate bottles of all series were withdrawn for analyses of the dissolved oxygen concentration at day 0 and after 7, 14, 21, and 28 exposure and the cumulative percentages biodegradation calculated from the oxygen depletion ratio BOD/ThOD. Minor deviations from the guideline included omitting ammonium chloride from the medium to prevent oxygen consumption due to nitrification and use of secondary activated sludge as inoculum instead of an effluent/extract/mixture. Also, the pH was 7.0 at the start of the test instead of the recommended pH of 7.4 ± 0.2. None of these deviations are however considered to have affected the overall reliability or outcome of the test. All validity criteria were met.
After the 28-day incubation period the substance was biodegraded 71%. The 10-day window criterion was not met. The reason for failing the 10-day window is that the test substance is a mixture of two isomers present at 70% and 30%, respectively. The degradation curve is therefore the sum of two growth curves whereas the 10-day time window criterion was developed on the assumption that a single compound is degraded according to a ‘standard’ growth curve in ready biodegradability tests. The time-window may therefore be ignored as pass/fail criterion and Myrac aldehyde be classified as readily biodegradable based on the biodegradation percentage of 71% at day 28 alone.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
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