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EC number: 947-782-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
In Chemico Skin Sensitisation: Direct Peptide Reactivity Assay (DPRA), OECD 442C.
The method is not applicable for the testing of substances of unknown or variable composition, complex reaction products or biological materials (UVCB substances) due to the unknown and/or variable composition of the test substance as the defined molar ratio of test chemical and peptide is needed for the assessment of the test results. Reaction products of stearic acid, diethylenetriamine and N-aminoethylethanolamine and acetic acid is a UVCB, therefore this test was not performed.
In Vitro Skin Sensitisation: ARE-Nrf2 Luciferase Test Method (KeratinoSensTM), OECD 442D.
A GLP compliant OECD 442D test was performed on Reaction products of stearic acid, diethylenetriamine and N-aminoethylethanolamine and acetic acid to assess its skin sensitisation potential. This study concluded the substance is not a skin sensitiser.
In Vitro Skin Sensitisation: Human Cell Line Activation Test (h-CLAT), OECD 442E.
Substances with Log Kow up to 3.5 can be tested whereas substances with Log Kow higher than 3.5 tend to produce negative results. For such substances positive results could be used to support the identification of a test chemical as sensitiser. Negative results should be considered as inconclusive.
Although Reaction products of stearic acid, diethylenetriamine and N-aminoethylethanolamine and acetic acid was determined to have a high Log Kow >6.5, a GLP compliant OECD 442E test was performed, yeilding positive results to support classification as a skin sensitiser.
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08/02/2018 - 03/04/2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 442D (In Vitro Skin Sensitisation: ARE-Nrf2 Luciferase Test Method)
- Deviations:
- no
- Remarks:
- see principles of method below for full details
- Principles of method if other than guideline:
- Deviations from the guidelines:
- Standard states MTT musted be used for an incubation period of 4 hours, solubilised overnight in 10% SDS and read at 690 nm, the study uses MTT for a 3 hr incubation period, which is solubilised in isopropanol and read at 570 nm.
- The standard states that cinnamic aldehyde must be used at 4 - 64 μM, whereas the study uses cinnamic aldehyde 8 - 128 μM. - GLP compliance:
- yes (incl. QA statement)
- Type of study:
- activation of keratinocytes
- Justification for non-LLNA method:
- The test (KeratinoSens) test is a method for which validation studies have been completed followed by an independent peer review conducted by the European Union Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and is considered scientifically valid when used as part of an integrated approach to testing to determine skin sensitisers and non-sensitisers for the purposes of hazard classification and labelling.
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 0001230562
- Expiration date of the lot/batch: 08/06/2019
- Purity test date: N/A
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Assumed to be stable
- Stability under test conditions:Assumed to be stable
- Solubility and stability of the test substance in the solvent/vehicle: Assumed to be stable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: Assumed to be stable
- Details on the study design:
- Skin sensitisation (In vitro test system) - Details on study design: Using a test system (KeratinoSens cell line (test system) derived from the HaCaT human keratinocytes) a luciferase reporter gene transcriptionally controlled by the Anti-oxidant Response Element from a gene that is known to be up regulated in contact with sensitisers was quantitatively measured. Experiments were performed in plates where cells were exposed to in individual concentrations of the test substance or appropriate control substances. Test concentrations were determined based on the solubility of the test item in ethanol (max. 20 mg/mL in Ethanol), and for subsequent dilution in cell culture medium a top concentration of 200 μg/mL) with 3 replicates per concentration per treatment.
- Positive control results:
- The positive control, 'Cinnamic Aldehyde' showed that there was a dose-dependent increase of induction, which was > 1.5 - fold in at least one concentration and the CV% of blank values was < 20% so was accepted. The positive control was positive at both 64 and 129 μM.
- Key result
- Run / experiment:
- other: Test Item
- Parameter:
- other: EC1.5
- Value:
- 1.5
- Vehicle controls validity:
- not valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- no indication of skin sensitisation
- Run / experiment:
- other: Test item
- Parameter:
- other: Imax
- Value:
- 0.854
- Vehicle controls validity:
- not specified
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- no indication of skin sensitisation
- Other effects / acceptance of results:
- ACCEPTANCE OF RESULTS:
- Acceptance criteria met for negative control: Not specified.
- Acceptance criteria met for positive control: Not all criteria met, however considered valid based on Positive Control results.
- Acceptance criteria met for variability between replicate measurements: No specified.
- Range of historical values if different from the ones specified in the test guideline: Same. - Interpretation of results:
- GHS criteria not met
- Conclusions:
- The Reaction products of stearic acid, diethylenetriamine and N-aminoethylethanolamine and acetic acid was classified as a Non-sensitiser to human skin as EC1.5 value value did not exceed the minimum threshold (1.5) and the Imax value was between 0.854 - 1.183.
- Executive summary:
The study assesses the In vitro sensitisation potential of Reaction products of stearic acid, diethylenetriamine and N-aminoethylethanolamine and acetic acid using the test system (KeratinSens test method). The study is GLP compliant and is performed according to the OECD 442D guidelines. The experiment use the quantification of luciferase expression in the cell reporter line to determine the response to the test item, a negative control and a positive control, reporting the EC1.5 and Imax values. In all test item experiments the EC1.5 value did not exceed the minimum threshold value (1.5) and the Imax value range was 0.854 - 1.183, which lead to conclusion that the test item is not a skin sensitiser (GHS criteria determined).
- Endpoint:
- skin sensitisation: in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23/01/2018 - 14/03/2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 442E (In Vitro Skin Sensitisation: human Cell Line Activation Test (h-CLAT)).
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- other: THP-1 cell surface marker expression
- Justification for non-LLNA method:
- The test method (h-CLAT) has been evaluated in a European Union Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM)-lead validation study and subsequent independent peer review by the EURL ECVAM Scientific Advisory Committee (ESAC) and was considered scientifically valid to be used as part of an Integrated Approach to Testing and Assessment (IATA) to support the discrimination between skin sensitisers and non-sensitisers for the purpose of hazard classification and labelling.
Note that for this method, test items with a Log Kow (octanol/water partition coefficient) of up to 3.5 have been tested successfully. However, test items with a Log Kow of greater than 3.5 can still be tested at lower soluble concentrations. In such a case, a negative result (non-sensitiser) should be considered inconclusive, whereas a positive result (sensitiser) could still be considered valid. - Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 0001230562
- Expiration date of the lot/batch: 08/06/2019
- Purity test date: N/A - Details on the study design:
- Skin sensitisation (In vitro test system)
- Details on study design: THP-1 cells were pre-cultured for either 48 or 72 hrs. Following this, the cells were dosed with the test item over an 8 dose range and incubated for 24 ±0.5hrs. The cells were then washed and stained with propidium iodide which allows for discrimination of live/dead cells by flow cytometry. The dose of test item that yields 75% cell viability (CV75) was calculated and taken forward for the next stage of testing. This dose finding assay was carried out over two independent runs.
THP-1 cells were pre-cultured again for 48hrs. Once the CV75 was determined, a narrower dilution series based around the CV75 value was produced for the test item. This dilution range was used to dose the cells again for 24 ±0.5hrs. The cells were then washed and stained with propidium iodide and also with antibodies that detect CD54 and CD86 expression as well as a negative control antibody. This allowed for discrimination of live/dead cells and also changes in CD54 and CD86 marker expression by flow cytometry.
Prior to the CV75 determination, the test item was assessed for solubility and was found to be soluble in Isopropanol at 25 mg/mL. - Positive control results:
- Positive control - CV75:
Complete RPMI culture medium containing 10% Human Serum, 0.05mM 2-mercaptoethanol and 0.4% DMSO was used for reference to the positive control that was solubilised in DMSO. Positive control was accepted as both criteria where met (cell viability ≥ 75% at the lowest dose and the highest test item concentration produced cytotoxicity).
Positive Control - CD54 and CD86 Expression:
Complete RPMI culture medium containing 10% Human Serum, 0.05mM 2-mercaptoethanol and 0.4% Isopropanol was used for reference to the test item that was solubilised in Isopropanol. Positive control was accepted as all criteria where met. - Run / experiment:
- other: 1
- Parameter:
- other: EC200
- Remarks:
- CD54
- Value:
- 16
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- positive indication of skin sensitisation
- Key result
- Run / experiment:
- other: 2
- Parameter:
- other: EC200
- Remarks:
- CD54
- Value:
- 26
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- positive indication of skin sensitisation
- Run / experiment:
- other: 1
- Parameter:
- other: EC150
- Remarks:
- CD86
- Value:
- 13.76
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- positive indication of skin sensitisation
- Key result
- Run / experiment:
- other: 2
- Parameter:
- other: EC150
- Remarks:
- CD86
- Value:
- 25
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- positive indication of skin sensitisation
- Interpretation of results:
- Category 1 (skin sensitising) based on GHS criteria
- Conclusions:
- The Reaction products of stearic acid, diethylenetriamine and N-aminoethylethanolamine and acetic acid was classified as a skin sensitiser to human skin as EC200 and EC150 values for CD54 and CD86 expression were 26 and 25 µg/mL, respectively.
- Executive summary:
The study assesses the in vitro sensitisation potential of Reaction products of stearic acid, diethylenetriamine and N-aminoethylethanolamine and acetic acid using the test system (h-CLAT test method). The study is GLP compliant and is performed according to the OECD Guideline 442E. The experiment uses the quantification of cytotoxic effects observed (CV75 assay) and cell surface marker expression on THP-1 cells (CD54 and CD86 assays) after 24-hour exporure to the test substance to determine the response. The relative fluorescence intensity (RFI) is used a measure of expression of CD54 and CD86, calculated from the results at test item doses in duplicate.
The expression of CD54, as measured by the RFI, crossed the threshold (RFI ≥200) in 3/8 doses in run 1 and 4/7 doses in run 2.
The expression of CD86, as measured by the RFI, crossed the threshold (RFI ≥150) in 7/8 doses in run 1 and 4/7 doses in run 2.
Final EC200 and EC150 values for CD54 and CD86 expression were 26 and 25 µg/mL, respectively.
As the CD54/CD86 expression crossed the thresholds, the test item is classified as a skin sensitiser. Cell viability did not fall below 50% at any of the test item concentrations and therefore the result is deemed to be valid.
- Endpoint:
- skin sensitisation: in chemico
- Data waiving:
- study technically not feasible
- Justification for data waiving:
- other:
- Justification for type of information:
- The Direct Peptide Reactivity Assay (DPRA) is not applicable for the testing of UVCB substances due to the unknown and/or variable composition as the defined molar ratio of test chemical and peptide is needed for the assessment of the test results. Reaction products of stearic acid, diethylenetriamine and N-aminoethylethanolamine and acetic acid is a UVCB, therefore this test was not performed.
Referenceopen allclose all
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
Justification for classification or non-classification
The skin sensitisation potential of Reaction products of stearic acid, diethylenetriamine and N-aminoethylethanolamine and acetic acid was assessed experimentally by two of the three available in vitro tests (OECD 442D and 442E). OECD 442C could not be performed as the substance is a UVCB. The results of OECD 442D were negative, whilst those of 442E were positive and therefore no overall conclusion on classification can be determined based on these two results alone.
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