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EC number: 947-716-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-10-09 to 2018-11-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- Quartz sand
- Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- nominal: 20 +/- 2 °C
measured: 21.2-22.7 °C - Moisture:
- Maximum water holding capacity: 34.9 ± 1.8 g/100 g DW
The soil was adjusted to approx. 45% of MWHC.
Soil moisture content in terms of percent MWHC before application: 41.6%
Moisture on day 0: 43.1-43.8%
Moisture on day 28: 42.9-433.6% - Organic carbon content (% dry weight):
- 0.465
- Details on test conditions:
- TEST SYSTEM
- Testing facility: DR.U.NOACK-LABORATORIEN
- Test container (type, material, size): Plastic boxes (volume 1.0 L, food grade) with perforated tops to enable gas exchange
- Amount of soil: 400 g soil DW
- No. of replicates per concentration: 3
- No. of replicates per control: 3
- Control: Untreated soil with 1% quartz sand (tested under the same conditions as the test replicates)
SOIL INCUBATION
- Method: bulk approach
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil: LUFA soil 2.3
- Geographical reference of sampling site (latitude, longitude):Offenbach, “rechts der Landauer Str.“ Nr. 826/7, Rheinland-Pfalz, Germany
- History of site: Cultures: 2014 - 2018: uncultivated
Fertilisation: 2015 - 2018: none, no organic fertilisation.
- Vegetation cover: Not specified
- Treatments with pesticides or fertilizers: No crop protection products applied during sampling year and 4 preceding years.
- Accidental contamination: No
- Depth of sampling: ca. 20 cm
- Soil texture
Sand:
2.0 - 0.63 mm: 2.7 ± 0.5 %
0.63 - 0.2 mm: 29.4 ± 1.2 %
0.2 - 0.063 mm: 25.2 ± 1.4 %
Silt:
0.063 - 0.02 mm: 18.7 ± 1.3 %
0.02 - 0.006 mm: 11.0 ± 0.7 %
0.006 - 0.002 mm: 5.3 ± 0.8 %
Clay:
< 0.002 mm: 7.6 ± 0.4 %
- Soil taxonomic classification: silty sand
- Soil classification system: acc. to German DIN classification
- pH (in water): 5.9+/- 0.5
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight): 7.18
- Maximum water holding capacity (in % dry weigth): 34.9+/- 1.8
- Cation exchange capacity: 7.3 +/- 1.1 meq/100 g
LUFA Speyer:
The soil was manually cleared of large objects and then sieved to a particle size of up to 2 mm (carried out by LUFA Speyer).
The maximal water holding capacity and the pH-value were determined.
Test facility:
The soil moisture content was determined.
The soil was adjusted to about 45 % of its maximal water holding capacity with demineralised water. Drying out of the soil was prevented by moistening with demineralised water as necessary.
The soil was checked for a detectable microbial biomass (result in terms of percentage of total organic carbon) and the amount of total inorganic nitrogen.
The soil amounts were amended with powdered lucerne-green-grass-meal (0.5 % of soil dry weight).
At experimental starting the soil was adjusted to approximately 42 % of its maximal water holding capacity. All replicates were checked once per week for water losses by evaporation (recommended maximum 5 %, actual 0.5 - 1.0 %). Compensation with demineralised water was done. Replicates were weighed for this procedure.
- Storage (condition, duration): The soil was stored for 63 days (2018-07-18 to 2018-09-19) in the dark at 4 ± 2 °C in a climatic room (TE1200, VIESSMANN).
- Initial microbial biomass as % of total organic C: 3.30
DETAILS OF PREINCUBATION OF SOIL (if any): Subsequently, the soil was pre-incubated at room temperature (ca. 20 °C) for 19 days (2018-09-19 to 2018-10-09) before experimental starting to guarantee a temperature adaptation of the micro-organisms.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Measurements of inorganic nitrate were carried out after 0, 7, 14 and 28 days. The pH values and water contents were determined after 0 and 28 days.The room temperature was measured and recorded continuously by a datalogger.
Determination of nitrate concentrations:
Nitrate was extracted from soil with a mineral salt solution. For the elimination of coloured organic matter in the extraction solution a cleaning step with solid phase extraction (SPE) cartridges was carried out. Thereafter, photometric determination took place.
VEHICLE CONTROL PERFORMED: No vehicle used
RANGE-FINDING STUDY: Yes, with 10, 100 and 1000 mg/kg soil dw. Based on the results the concentrations for the definitive study were selected. - Nominal and measured concentrations:
- Nominal test concentrations: 1000 - 316 - 100 - 31.6 - 10.0 mg/kg soil dry weight (factor 3.16).
- Reference substance (positive control):
- yes
- Remarks:
- Cyanoguanidine, conducted from 2018-05-18 to 2018-06-15 with 50 mg/kg soil dry weight and resulted in 33% compared to control on the basis of Nitrate-N.
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- 19.8 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Duration:
- 28 d
- Dose descriptor:
- EC25
- Effect conc.:
- 82 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- 274 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes,
- Relevant effect levels: difference to control > 25 % - Validity criteria fulfilled:
- yes
- Conclusions:
- Based on results of an OECD 216 compliant study with the test item an EC10 (based on the Nitrate-N formation rate) of 19.8 mg/kg soil dw was determined as key value.
- Executive summary:
The effects of the test item on the metabolic activity of soil micro-organisms were determined according to OECD Guideline 216 (2000) over 28 days. The test item was applied via quartz sand with the test concentrations 1000 - 316 - 100 - 31.6 - 10 mg/kg soil dry weight. Untreated soil was tested as control under the same test conditions as the test item concentrations. Standard soil, moistened to nominally 45 % of its maximal water holding capacity, was used. Plastic boxes (volume 1.0 L) with perforated tops to enable gas exchange and filled with 400 g soil dry weight were used. The effects of the test item on the metabolic activity of the nitrogen-N formation rate (nitrate) were measured on the day of treatment (day 0) and after 7, 14 and 28 days.
After 7 days of exposure, no significant inhibition of the microbial nitrate transformation was observed for all test item concentrations up to 1000 mg/kg soil dry weight. After 14 days of exposure, a significant inhibition of the microbial nitrate transformation was observed for test item concentrations 100 mg/kg soil dry weight. After 28 days of exposure a significant inhibition of the microbial nitrate transformation was observed for test item concentrations > 100 mg/kg soil dry weight. For the test item concentration 1000 mg/kg soil dry weight, nitrate formation rates were negative, due to low nitrate contents. On day 28 the NOEC for nitrogen transformation in soil measured as nitrate was 31.6 mg/kg soil dry weight, the EC10 for nitrogen transformation in soil measured as nitrate was 19.8 mg/kg soil dry weight.
Reference
Nitrate-N Contents -Day 0
Test concentration |
Nitrate-N |
||
Repl. |
MV±SD |
CV |
|
[mg NO3-N/kg soil dry weight] |
[%] |
||
Control |
26.5 |
26.5 ± 0.10 |
0 |
26.6 |
|||
26.4 |
|||
10.0 |
27.8 |
27.2 ± 0.60 |
2 |
26.6 |
|||
27.2 |
|||
31.6 |
26.6 |
27.3 ± 0.82 |
3 |
27.1 |
|||
28.2 |
|||
100 |
28.5 |
27.8 ± 0.96 |
3 |
28.2 |
|||
26.7 |
|||
316 |
29.1 |
29.1 ± 2.05 |
7 |
31.2 |
|||
27.1 |
|||
1000 |
26.7 |
27.4 ± 1.33 |
5 |
28.9 |
|||
26.5 |
Nitrate-N Contents -Day 7
Test concentration |
Nitrate-N |
Nitrate-N Formation Rate |
||||
Repl. |
MV±SD |
CV |
Repl. |
MV±SD |
CV |
|
[mg NO3-N/kg soil dry weight] |
[%] |
[mg NO3-N×(kg soil dry weight×d)-1] |
||||
Control |
34.0 |
35.3 ± 2.25 |
6 |
1.07 |
1.26 ± 0.32 |
26 |
37.9 |
1.63 |
|||||
34.0 |
1.07 |
|||||
10.0 |
37.6 |
35.6 ± 2.16 |
6 |
1.49 |
1.20 ± 3.11 |
26 |
35.8 |
1.23 |
|||||
33.3 |
0.87 |
|||||
31.6 |
34.5 |
36.0 ± 1.31 |
4 |
1.03 |
1.24 ± 0.19 |
15 |
37 |
1.39 |
|||||
36.4 |
1.30 |
|||||
100 |
36.3 |
35.4 ± 0.78 |
2 |
1.21 |
1.08 ± 0.11 |
10 |
35.0 |
1.03 |
|||||
34.9 |
1.01 |
|||||
316 |
37.3 |
36.9 ± 0.55 |
1 |
1.17 |
1.12 ± 0.08 |
7 |
36.3 |
1.03 |
|||||
37.2 |
1.16 |
|||||
1000 |
35.4 |
36.6 ± 1.11 |
3 |
1.14 |
1.31 ± 0.16 |
12 |
37.6 | 1.46 | |||||
36.8 | 1.34 |
Nitrate-N Contents -Day 14
Test concentration |
Nitrate-N |
Nitrate-N Formation Rate |
||||
Repl. |
MV±SD |
CV |
Repl. |
MV±SD |
CV |
|
[mg NO3-N/kg soil dry weight] |
[%] |
[mg NO3-N×(kg soil dry weight×d)-1] |
||||
Control |
42.8 |
44.3 ± 1.97 |
4 |
1.16 |
1.27 ± 0.14 |
11 |
43.5 |
1.21 |
|||||
46.5 |
1.43 |
|||||
10.0 |
47.7 |
45.2 ± 1.35 |
3 |
1.39 |
1.29 ± 0.09 |
7 |
44.1 |
1.21 |
|||||
44.8 |
1.26 |
|||||
31.6 |
44.7 |
44.2 ± 1.61 |
4 |
1.24 |
1.21 ± 0.11 |
9 |
42.4 |
1.08 |
|||||
45.5 |
1.30 |
|||||
100 |
40.6 |
40.9 ± 1.82 |
4 |
0.91 |
0.94 ± 0.13 |
14 |
42.9 |
1.08 |
|||||
39.3 |
0.82 |
|||||
316 |
35.6 |
34.6 ± 0.91 |
3 |
0.46 |
0.40 ± 0.07 |
16 |
33.8 |
0.34 |
|||||
34.5 |
0.39 |
|||||
1000 |
28.3 |
29.8 ± 1.41 |
5 |
0.06 |
0.17 ± 0.10 |
59 |
30.0 | 0.19 | |||||
31.1 | 0.26 |
Nitrate-N Contents -Day 28
Test concentration |
Nitrate-N |
Nitrate-N Formation Rate |
||||
Repl. |
MV±SD |
CV |
Repl. |
MV±SD |
CV |
|
[mg NO3-N/kg soil dry weight] |
[%] |
[mg NO3-N×(kg soil dry weight×d)-1] |
||||
Control |
57.6 |
57.9 ± 0.31 |
6 |
1.11 |
1.12 ± 0.01 |
26 |
58.0 |
1.13 |
|||||
58.2 |
1.13 |
|||||
10.0 |
57.1 |
57.1 ± 1.65 |
6 |
1.07 |
1.07 ± 0.06 |
26 |
58.8 |
1.13 |
|||||
55.5 |
1.01 |
|||||
31.6 |
54.4 |
54.9 ± 0.92 |
4 |
0.97 |
0.99 ± 0.004 |
15 |
56.0 |
1.03 |
|||||
54.4 |
0.97 |
|||||
100 |
46.7 |
48.1 ± 2.42 |
2 |
0.68 |
0.73 ± 0.009 |
10 |
46.7 |
0.68 |
|||||
50.9 |
0.83 |
|||||
316 |
51.0 |
45.0 ± 5.23 |
1 |
0.78 |
0.57 ± 0.19 |
7 |
42.6 |
0.48 |
|||||
41.4 |
0.44 |
|||||
1000 |
17.9 |
16.9 ± 1.18 |
3 |
n.d. |
- |
- |
15.6 | n.d. | |||||
17.2 | n.d. |
Repl.: Replicate MV: Mean value
SD: Standard deviation CV: Coefficient of variation
n.d. not determinable, due to negative Nitrate-N Formations rates
Description of key information
Based on results of an OECD 216 compliant study with the test item an EC10 (based on the Nitrate-N formation rate) of 19.8 mg/kg soil dw was determined as key value for the risk assessment.
Key value for chemical safety assessment
- Short-term EC50 for soil microorganisms:
- 274 mg/kg soil dw
- Long-term EC10 or NOEC for soil microorganisms:
- 19.8 mg/kg soil dw
Additional information
The effects of the test item on the metabolic activity of soil micro-organisms were determined according to OECD Guideline 216 (2000) over 28 days. The test item was applied via quartz sand with the test concentrations 1000 - 316 - 100 - 31.6 - 10 mg/kg soil dry weight. Untreated soil was tested as control under the same test conditions as the test item concentrations. Standard soil, moistened to nominally 45 % of its maximal water holding capacity, was used. Plastic boxes (volume 1.0 L) with perforated tops to enable gas exchange and filled with 400 g soil dry weight were used. The effects of the test item on the metabolic activity of the nitrogen-N formation rate (nitrate) were measured on the day of treatment (day 0) and after 7, 14 and 28 days.
After 7 days of exposure,no significant inhibition of the microbial nitrate transformation was observed for all test item concentrations up to 1000 mg/kg soil dry weight. After 14 days of exposure,a significant inhibition of the microbial nitrate transformation was observed for test item concentrations 100 mg/kg soil dry weight. After 28 days of exposurea significant inhibition of the microbial nitrate transformation was observed for test item concentrations > 100 mg/kg soil dry weight. For the test item concentration 1000 mg/kg soil dry weight, nitrate formation rates were negative, due to low nitrate contents.On day 28 the NOEC for nitrogen transformation in soil measured as nitrate was 31.6 mg/kg soil dry weight, the EC10 for nitrogen transformation in soil measured as nitrate was 19.8 mg/kg soil dry weight.
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