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EC number: 205-472-3 | CAS number: 141-24-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 Aug - 01 Sep 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with restrictions (no analytical monitoring), only three replicates used.
- Qualifier:
- according to guideline
- Guideline:
- other: Annex of EU guideline 92/69/EWG, adopted 1992
- Qualifier:
- according to guideline
- Guideline:
- other: VCI concept draft for (acute) aquatic testing of poorly soluble substances, adopted 1994
- Principles of method if other than guideline:
- The VCI concept schedules a maximum of 4 test steps:(1) Limit test at nominal 100 mg/L without separation of phases(2) Limit test at nominal 100 mg/L after separation of insoluble components(3) Testing at saturation level: 3- to 5-fold saturation concentration and separation of insoluble components(4) Testing of dilutions of the saturated solution prepared in (3)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method 1: 100 mg/L after separation of undissolved material:111 mg test substance were added to 1 L deionised water and continuously stirred for 24 h using a magnetic stirrer. Insoluble parts of the test substance were separated by filtration through a glass fibre filter pre-treated with 1 M NaOH.- Method 2: 100 mg/L direct weighing without separation of undissolved material:After weighing, the test substance solution was incubated for 24 h on a shaker.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM- Source (laboratory, culture collection): Laboratory stock at Henkel KGaA, originally obtained from Universität Göttingen, Institute of Plant Physiology- Age of inoculum (at test initiation): 3 to 4 days pre-incubation in test medium without test substanceACCLIMATION- Culturing media and conditions (same as test or not): same
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Hardness:
- no data
- Test temperature:
- 23 °C
- pH:
- Method 1: 6.9 - 7.0 at test startMethod 2: 6.8 - 7.2 at test end
- Dissolved oxygen:
- no data
- Nominal and measured concentrations:
- Nominal test substance concentration: Method 1: 100 mg/L (with filtration)Method 2: 100 mg/L (without filtration)
- Details on test conditions:
- TEST SYSTEM- Test vessel: Erlenmeyer flasks- Size, fill volume: 300 mL, 100 mL- Initial cells density: 10,000 cells/mL- Control end cells density: 220,000 cells/mL- No. of vessels per concentration (replicates): 3- No. of vessels per control (replicates): 3GROWTH MEDIUM- Standard medium used: mineral salt medium following DIN 38412/9- Detailed composition if non-standard medium was used: no details givenOTHER TEST CONDITIONS- Sterile test conditions: Bacterial growth was observed in the test series with direct weighing of the test substance.- Photoperiod: continuous illumination- Light intensity and quality: max. 300 lux with daylight spectrum- Shaking: 120 rpmEFFECT PARAMETERS MEASURED (with observation intervals if applicable) :- Determination of cell concentrations: counting chamber
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- growth rate
- Remarks on result:
- other: Method 1: with filtration
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- < 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Method 2: without filtration
- Details on results:
- - Exponential growth in the control (for algal test): yes- Other: In the test series with direct weighing of the test substance, turbidity of the medium occured due to growth of bacteria- Effect concentrations exceeded solubility of substance in test medium in part 2 of the study.The decrease in the algal growth observed in the replicates without filtration are presumably due to physical effects.
Reference
Algal growth rate:
Method 1 (with filtration): average growth inhibition: 0%
Method 2 (without filtration): average growth inhibition: 61.2%
Algal biomass:
Method 1 (with filtration): average growth inhibition: 0%
Method 2 (without filtration): average growth inhibition: 92.7%
Part 2 of the study (after separation of undissolved test substance): No negative effects at the level of water solubility (< 0.025 mg/L).
Based on these results it can be assumed that the effects occurring in the presence of undissolved test substance components are due to the physical effects of undissolved fractions rather than to systemic toxicity.
Table 1: Cell concentrations after 24, 48 and 72 h of exposure to the test substance
Test Concentration (mg/L) | Cell Concentration (cells/mL) | ||
24 h | 48 h | 72 h | |
0 | 3.0E04 | 9.0E04 | 2.2E05 |
100 (method 2) | 0 | 2.3E04 | 3.3E04 |
100 (method 1) | 2.0E04 | 1.5E05 | 3.6E05 |
Description of key information
No effects up to the limit of water solubility
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
Additional information
Experimental data on the acute toxicity of methyl ricinoleate (CAS 141-24-2) to aquatic algae are not available. Therefore, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5, toxicity data from structurally related substance Fatty acids, C16-18 and C18 unsaturated isobutyl esters (CAS No. 84988-79-4) was used to assess the toxicity to algae in a read across approach.
Based on the high degree of similarity between the structural and physico-chemical properties of the target and source substance, the source substance is considered as a suitable representative for the evaluation of the short-term toxicity of the target substance to aquatic invertebrates. The read-across approach is justified in detail within the analogue justification in IUCLID section 13.
The study on the read across substance Fatty acids, C16-18 and C18 unsaturated isobutyl esters (CAS No. 84988-79-4) was performed as a limit test according to the EU guideline 92/69/EWG and GLP (BASF, 1995). The test organism Scenedesmus subspicatus was exposed to the test substance in a static system for 72 hours, at the nominal test concentration 100 mg/L. The test was performed with and without separation of undissolved test material (the test substance was separated by filtration). With undissolved test substance present, algal growth was significantly inhibited. An inhibition of the algal growth was not observed in the filtered test solutions. The inhibition can be thus regarded as physical effect caused by undissolved material. It can be concluded that the substance has no toxic effects on test organism up to the limit of water solubility.
Based on the results obtained for the structurally related read across substance an acute toxicity of methyl ricinoleate (CAS 141-24-2) to aquatic algae is not expected within the limit of its water solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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