Pyridine-2,6-dicarboxylic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Pyridine-2,6-dicarboxylic acid

EC Number:

207-894-3

EC Name:

Pyridine-2,6-dicarboxylic acid

Cas Number:

499-83-2

Molecular formula:

C7H5NO4

IUPAC Name:

pyridine-2,6-dicarboxylic acid

Test material form:

solid: crystalline

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

A solution containing 100.5 mg/L test item in dilution water was prepared and treated with ultrasonication. In the non-GLP pre-test, pH of the test item solution was 3.5, therefore the pH was adjusted to 7.9 (as in the dilution water) and then the test item solution was used for the test.
This procedure is in agreement with the OECD guidance document no. 23.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Species Daphnia magna
Authority STRAUS
Strain Berlin
Sex female
Age between 0 and 24 hours
Origin Umweltbundesamt Berlin
In-house breeding since 27. September 2007
Selection of the test system was made following the proposal of the guidelines

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.502 mmol/L

Test temperature:

19.8 – 21.6°C

pH:

7.8

Dissolved oxygen:

8.9 mg/L

Nominal and measured concentrations:

The measured concentration at the beginning of the test was at 102.39% of the nominal concentration and after 48 hours the measured concentration was at 114.58% of the nominal concentration. Therefore, the determination of the biological results was based on the nominal concentration.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 4

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

1.1     Biological Results Test Item

The biological results are presented in the following table:

Table 9.3‑a      Biological Results Test Item

Parameter	Value
24h EC50	> 100 mg/L
48h EC50	> 100 mg/L
48h NOEC	≥100 mg/L
48h LOEC	> 100 mg/L

2      Validity

¨            Immobilisation in the controls may not exceed 10 %.
Immobilisation in the controls was 0 %.

¨            The concentration of dissolved oxygen at the end of the test must be at least 3 mg/L. The lowest concentration of dissolved oxygen at the end of the test was 8.9 mg/L.

One valid experiment was performed.

The study was performed as a limit test with 100 mg/L. For the test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.

Toxicity was not observed in the treatment and also none of the animals was immobilised in the blank control.

Potassium dichromate K₂Cr₂O₇ (CAS No. 7778-50-9) was used as positive controlin a current reference study to assure that the test conditions are reliable.

 

At the beginning and at the end of the test, the content of the test item in the test solutions was determined usingHPLC-UV-determination. The measured concentration at the beginning of the test was at 102.39% of the nominal concentration and after 48 hours the measured concentration was at 114.58% of the nominal concentration. Therefore, the determination of the biological results was based on the nominal concentration.

No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met.

The result of the test is considered valid. 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

48h-NOEC≥100 mg/L
48h-LOEC > 100 mg/L
24h-EC50> 100 mg/L
48h-EC50> 100 mg/L

Executive summary:

One valid experiment was performed.

The study was performed as a limit test with 100 mg/L. For the test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.

Toxicity was not observed in the treatment and also none of the animals was immobilised in the blank control.

Potassium dichromate K₂Cr₂O₇ (CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable.

 

At the beginning and at the end of the test, the content of the test item in the test solutions was determined using HPLC-UV-determination. The measured concentration at the beginning of the test was at 102.39% of the nominal concentration and after 48 hours the measured concentration was at 114.58% of the nominal concentration. Therefore, the determination of the biological results was based on the nominal concentration.

The following results were determined for the test itemDipicolinic acid (DPA)(species:Daphnia magna).

 

48h-NOEC≥100 mg/L
48h-LOEC > 100 mg/L
24h-EC₅₀> 100 mg/L
48h-EC₅₀> 100 mg/L