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EC number: 215-671-7 | CAS number: 1340-06-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- start of study: November 4th, 1993
termination of study: November 5th, 1993
duration of study 16 +- 1 h - Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Deviations:
- not specified
- Principles of method if other than guideline:
- Determination of the inhibitory effect of the sample on Pseudomonas putida by measurement of cell growth under the influence of varying dilutions of the test sample, compared to the cell growth of a culture obtained under the same conditions, but without the test sample.
Determination of the cell concentration as optical density after a test period of 16 h ± 1 h.
The concentrations of the test sample at which cell multiplication is inhibited by 10 % and 50 % within 16 h ± 1 h. are the basis for assessment. - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ichthyolic acid, sodium salt
- EC Number:
- 215-671-7
- EC Name:
- Ichthyolic acid, sodium salt
- Cas Number:
- 1340-06-3
- IUPAC Name:
- Identification of the UVCB substance by "Chemcial Abstracts Index Name", among others: Ichthyolic Acid, Sodium Salt. No IUPAC name known.
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- The test substance was taken from a regular production batch of the registrant's manufacturing site (sulfonated shale oil, pale batch no. R 92/0541). The name "sulfonated shale oil, pale is a common general expression for the trademark substance ICHTHYOL PALE. A certificate of analysis is part of the study report.
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Test solutions consisted of inoculation medium, stock solutions and test substance solution. For preparation of the different solutions aqua dest. was used.
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- One day old stock cultures of Pseudomonas putida served as inocula for preliminary cultures. Using aseptic techniques, suspensions of bacteria in preliminary culture medium (stock solution I, III, IV; 2.5 ml stock solution I, 2.5 ml stock solution III, 5.0 ml stock solution IV; see below) were diluted to a concentration corresponding a turbidity value of TU/F = 10 (turbidity units formazin).
Stock solution I
10.0 g sodium nitrate
2.4 g dipotassium hydrogen phosphate
1.2 g potassium dihydrogen phosphate
1.0 g yeast extract
in 500 ml aqua dest.
Stock solution II
10.0 g sodium nitrate
2.4 g dipotassium hydrogen phosphate
1.2 g potassium dihydrogen phosphate
in 500 ml aqua dest.
Stock solution III
44.0 g D(+)-glucose-monohydrate
in 500 ml aqua dest.
Stock solution IV
0.01 g ferrous citrate, w(Fe) = 19%
4.0 g magnesium sulphate, heptahydrate
in 1000 ml aqua dest.
Four parallel dilution series in 250-ml Erlenmeyer flasks with sterile stoppers were prepared with a final volume of 100 ml test medium each. Three series were inoculated with bacteria. The fourth series was not inoculated and served as a blank.
The final volume consisted of 10 ml of the inoculation medium (or 10 ml of preliminary culture medium for the fourth series which was not inoculated), 10 ml of different stock solutions and 80 ml of the test substance solution. The dilution factor was 2.0. The bacterial concentration at start corresponded to a turbidity value of TU/F = 5 (TU/F: turbidity units formazin). The pH was 6.94 to 6.95.
Four control culture flasks with 80 ml sterile water, 2.5 ml of stock solution and 10 ml of preliminary culture medium (1 flask) were also incubated.
Both inoculated an non-inoculated dilution series as well as the control culture flasks were left at 20°C - 21°C in the dark for 16 +- 1 hours (shaker; 75 - 85 rpm).
After termination of the test period the extinction of the monochromatic light at 436 nm was measured in a 10-mm cell with samples from the four test substance flasks per concentration and the control flasks. Since turbidity occurred in the dilution series for chemical-physical reasons, the analogous steps of dilution of the non-inoculated series were used as photometric blank values for the turbidity of the inoculated dilution series.
Study design
- Test type:
- not specified
- Water media type:
- other: The water media type follows the provisions of the method: surface, ground, or waste water to be tested.
- Limit test:
- yes
- Total exposure duration:
- 16 h
- Remarks on exposure duration:
- according to guideline requirements
- Post exposure observation period:
- according to guideline requirements
Test conditions
- Hardness:
- not applicable
- Test temperature:
- 20°C - 21°C
- pH:
- Initial pH of all test compound solutions and the control solution: 6.94 - 6.95
- Dissolved oxygen:
- not applicable
- Salinity:
- not applicable
- Conductivity:
- not applicable
- Nominal and measured concentrations:
- Nominal concentrations apply according to study report information. Preparation of the inoculation medium is described accordingly.
- Details on test conditions:
- Both inoculated and non-inoculated dilution series as well as the control culture flasks were left at 20°C - 21°C in the dark for 16 +- 1 hours (shaker: 75 - 85 rpm).
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- After termination of the test period the extinction of the monochromatic light at 436 nm was measured in a 10 mm cell with samples from the four test substance flasks per concentration and the control flasks. Since turbidity occured in the diluiton series for chemical-physical reasons, the analogous steps of dilution of the non-inoculated series were used as photometric blank values for the turbidity of the inoculated dilution series.
- Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- The following values were calculated
(i) mean extinction value of the control cultures
(ii) mean extinction values of each concentration of the inoculated dilution series
The mean extinction values of each dilution step were plotted against the logarithm of the values of the concentration of the test substance. The extinction values were corrected for turbidity.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Sulfonated Shale Oil, Pale (ICHTHYOL PALE) was investigated for its ability to inhibit the cell multiplication of the bacteria species Pseudomonas putida. Cultures of the bacteria were exposed to concentrations ranging from 625 mg to 10000 mg test substance/l.
Under the present test conditions no inhibition of cell multiplication of the bacteria species Pseudomonas putida was observed. On the contrary, the sulfonated shale oil, pale (ICHTHYOL PALE) appeared to have a nutritive effect.
The highest tested concentration which is equivalent to the highest reasonable concentration (limit test) was 10000 mg test substance/l.
The following parameters were determined for Pseudomonas putida:
EC10(16 h) : > 10000 mg sulfonated shale oil, pale/l
EC50(16 h): > 10000 mg sulfonated shale oil, pale/l
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