Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 812-497-9 | CAS number: 1893414-79-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name: Phosphoric acid, C14-15 branched and linear alkyl esters, potassium salts
CAS No.: 1893414-79-3
Physical state: white solid at 20 °C
Batch No.: PU61810016
Re-certification date of batch: 09 March 2018
Purity: 100 % (UVCB, lyophilized solid, water content 0.85 % (w/w))
Stability: stable under test conditions
Storage condition of test material: Room temperature, protected from light - Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge from the municipal wastewater treatment plant of 75175 Pforzheim, Germany, collected from the aeration tank was used as the microbial inoculum for the test. This plant was predominantly treating domestic sewage. The sludge was used one day after collection. It was settled and the upper layer with finer solids was decanted and discarded. Before starting the test, the remaining sludge was washed two times with chlorine free tap water by centrifugation (10 minutes at 3000 rpm equals 1966 g). After centrifuging, the supernatant was decanted and discarded and the sludge was resuspended in chlorine free tap water. This procedure was repeated once. The mixed liquor suspended solids (MLSS) in the sludge inoculum were adjusted to a concentration of 3.0 g/L (± 10 %). The activated sludge was continuously aerated at the test temperature, the solids did not settle down.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Hardness:
- The test was performed in a synthetic sewage feed that was prepared with the following amounts of substances in 1 litre of deionised water:
16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCl, 0.4 g CaCl2 × 2 H2O, MgSO4 × 7 H2O, 2.8 g K2HPO4. - Test temperature:
- Temperature in the main test: 18.4 – 20.8 °C (measured during O2-determination after 3 h of exposure)
- pH:
- pH of all test item solutions, the controls and reference item in the main test: 7.31 – 7.94
- Dissolved oxygen:
- The test solutions without microbial inoculum were aerated for some minutes to saturate the solutions with O2 above 60 - 70 %. Then the test was started (t0 = 0 hours) by adding 250 mL of microbial inoculum. During the incubation period of 3 hours all test solutions were continuously aerated by compressed air.
- Salinity:
- The test was performed in a synthetic sewage feed that was prepared with the following amounts of substances in 1 litre of deionised water:
16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCl, 0.4 g CaCl2 × 2 H2O, MgSO4 × 7 H2O, 2.8 g K2HPO4. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (DCP)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- The EC50 of the total oxygen uptake for DCP was estimated (following the normal distribution) to be 2.11 mg/L. This is within the accepted range of 2 to 25 mg/L for total respiration.
- Reported statistics and error estimates:
- The statistical evaluation was performed for the respiration rate of the control and the test highest item concentration using SAS® (2002–2010). The data were tested on normal distribution by the Shapiro-Wilk’s statistics and for homogeneity of variance by using F-test. The NOEC and LOEC were determined by using the Satterthwaite t-test. The statistical EC50 estimation of the toxic reference item was performed using probit analysis following the normal distribution.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a Klimisch 1 study according to OECD 209 guideline the test item had no statistically significant inhibitory effect on the total oxygen uptake of activated sludge microorganisms after 3 hours. The NOEC is therefore determined to be 1000 mg/L. All validity criteria according to OECD Guideline for Testing Chemicals 209 were fulfilled.
- Executive summary:
In a Klimisch 1 study according to OECD 209 guideline the test item had no statistically significant inhibitory effect on the total oxygen uptake of activated sludge microorganisms after 3 hours. The NOEC is therefore determined to be 1000 mg/L. The EC50 for total oxygen uptake was above the highest test item concentration of 1000 mg/L. All validity criteria according to OECD Guideline for Testing Chemicals 209 were fulfilled.
Reference
Validity Criteria of the Study
Control oxygen uptake:
The oxygen uptake rate RS in the blank controls (without test item or reference substance) should be not less than 20 mg O2/(g×h). In this study the mean control oxygen uptake was determined to be 24 mg O2/(g×h) after 3 hours.
Coefficient of variation:
The coefficient of variation of the total oxygen uptake rate in control replicates should be ≤ 30% at the end of the test. In this test, the coefficient of variation was 9 % after 3 hours.
Reference item EC50:
The EC50 of the total oxygen uptake for DCP was estimated (following the normal distribution) to be 2.11 mg/L. This is within the accepted range of 2 to 25 mg/L for total respiration.
Biological Results
The specific respiration rate of the range-finding test in a limit-test design and the percentage inhibition are summarised in below. No statistical significant differences to the control were determined up to 1000 mg/L after 3 hours for the total oxygen uptake so no main test was required. The NOEC was therefore determined to be at 1000 mg/L. The EC50 for total oxygen uptake was above 1000 mg/L. No chemical oxygen depletion was observed in the abiotic control. The EC50 of the total oxygen uptake for DCP was estimated (following the normal distribution) to be 2.11 mg/L. This is within the accepted range of 2 to 25 mg/L for total respiration.
Specific respiration rate and inhibition of total oxygen uptake
Test assay nominal [mg/L] |
Mean specific respiration rates after 3 h [mg O2/(g × h)] |
Inhibition after 3 h [%] |
Control (mean) | 23.97 | - |
10.0 | 22.17 | 7.5 |
100 | 23.61 | 1.5 |
1000 (mean of three replicates) | 20.98 | 12.5 |
DCP 0.2 | 18.31 | 23.6 |
DCP 1 | 13.93 | 41.9 |
DCP 5 | 10.76 | 55.1 |
DCP 25 | 4.42 | 81.6 |
Description of key information
In a Klimisch 1 study according to OECD 209 guideline the test item had no statistically significant inhibitory effect on the total oxygen uptake of activated sludge microorganisms after 3 hours. The NOEC is therefore determined to be 1000 mg/L. All validity criteria according to OECD Guideline for Testing Chemicals 209 were fulfilled.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.