Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 257-885-3 | CAS number: 52372-39-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the skin sensitization potential of the test chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1). The studies are as mentioned below:
The peer reviewed journal conductedskin sensitization study for read across substance in 10 female guinea pigs by using modified FCA and guinea pig maximization test.In the induction phase, the test compound was dissolved in FCA, 15 mg in 4 ml. Then physiological saline was added and an emulsion was prepared by mixing the material by mean of a 5ml syringe, until emulsification was completed.The intradermal injections of 6 X 0.10-0.15 ml of this emulsion was applied in a semi-circle arc on clipped and shaved shoulder from left to right in such a way that whole quantity of emulsion was used up for 10 animals. This procedure was repeated on 5thand 9thday. , leaving a gap of 2-3 cm between the rows of injection.After induction phase of 11 days, animals were challenged by applying 0.05ml of test chemical in a subirritant concentration, with further dilution to the clipped and shaved skin of the right flank of the treated animals, allowing 5 mins for drying. Control animals were also used.Mean Observations were made after 24, 48 and 72 hours. As no sensitization effects were observed, the test material can be concluded as non-sensitizing to the female guinea pigs.
The above result was further supported by the skin sensitization study reported by safety assessment reportfor another read across chemicalin guinea pigs. In this study, the test animals were treated dermally with 10% solution of test chemical during induction. After induction, treated animals were dermally exposed to challenge concentration of 1 or 2% solutions of test chemical. Since the test animals failed to induce any cutaneous reaction at challenge concentrations, the test chemical was considered as not sensitizing to guinea pigs.
Based on the above summarized studies for target chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) and its structurally and functionally similar read across substances,it can be concluded that the testchemical is unable to cause skin sensitization and considered as non-skin sensitizer. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally and functionally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on 2 skin sensitization studies as- WoE-2 and WoE-3.
Skin sensitization test was conducted on guinea pigs to determine the potential of skin sensitization caused by the chemical. - GLP compliance:
- not specified
- Type of study:
- other: 1. Modified FCA method and Guinea pig maximization test 2.Not specified
- Justification for non-LLNA method:
- not specified
- Specific details on test material used for the study:
- - Name of test material : 3-(diethylamino)-7-imino-7H-[1]benzopyrano[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile
- Molecular formula : C23H19N5O
- Molecular weight : 381.437 g/mol
- Smiles notation : c12cc3c4nc5ccccc5n4c(=N)c(C#N)c3oc1cc(N(CC)CC)cc2
- InChl : 1S/C23H19N5O/c1-3-27(4-2)15-10-9-14-11-16-21(29-20(14)12-15)17(13-24)22(25)28-19-8-6-5-7-18(19)26-23(16)28/h5-12,25H,3-4H2,1-2H3
- Substance type : Organic
- Physical state : Solid - Details on the study design:
- 1.TEST ANIMALS
- Source: No data available
- Age at study initiation: No Data available
- Weight at study initiation:280-350 g
- Housing: animals were kept 3 to a cage
- Diet (e.g. ad libitum):altromin ad libitum
- Water (e.g. ad libitum): water ad libitum
- Acclimation period: No Data available
ENVIRONMENTAL CONDITIONS
- Temperature (°C):22-24⁰C
- Humidity (%):50-55%
- Air changes (per hr): No Data available
- Photoperiod (hrs dark / hrs light): Under artificial illumination for 10 hours a day
2.not specified - Species:
- guinea pig
- Strain:
- other: 1.Pirbrigjht white strain 2.not specified
- Sex:
- female
- Details on test animals and environmental conditions:
- not specified
- Route:
- intradermal
- Vehicle:
- other: FCA and saline
- Concentration / amount:
- 15 mg
- Day(s)/duration:
- 5th and 9th day
- Adequacy of induction:
- other: WoE-1
- Route:
- other: Dermal
- Vehicle:
- not specified
- Concentration / amount:
- 10%
- Day(s)/duration:
- not specified
- Adequacy of induction:
- other: WoE-2
- No.:
- #1
- Route:
- epicutaneous, open
- Vehicle:
- other: Acetone
- Concentration / amount:
- 0.05ml
- Day(s)/duration:
- 72 hours
- Adequacy of challenge:
- other: WoE-1
- No.:
- #1
- Route:
- other: Dermal application
- Vehicle:
- not specified
- Concentration / amount:
- 1 or 2%
- Day(s)/duration:
- not specified
- Adequacy of challenge:
- other: WoE-2
- No. of animals per dose:
- 1. Induction route-10
Challenge route-10
1.No data available. - Details on study design:
- 1.MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: 8
- Exposure period:9 days
- Test groups: 10 guinea pigs
- Control group: 10 guinea pigs
- Site: Intradermal induction -Intraderma injection of 6x0.10-0.15 ml of emulsion were applied.The emulsion was applied in a semicircular arc on clipped and shaved shoulder area (4Х6 cm) from left to right.
- Frequency of applications: repeated on 5th and 9th days
- Duration: 9 days
- Concentrations: 15 mg in 4 ml FCA
B. CHALLENGE EXPOSURE
- No. of exposures: multiple times
- Day(s) of challenge: 11 days after induction, animals were challenged.
- Exposure period:5 mins
- Test groups: 10 guinea pigs
- Control group: 10 guinea pigs
- Site: clipped and shaved skin of the right flank of treated animals.
- Concentrations:0.05ml
- Evaluation (hr after challenge): after 24, 48 and 72 hr.
2.
MAIN STUDY
A. INDUCTION EXPOSURE
- Concentrations: 10%
B. CHALLENGE EXPOSURE
- Concentrations: 1or 2%
-
- Challenge controls:
- 1.No data available.
2.not specified - Statistics:
- 1.Mean response was computed as the quotient of the sum of the observe reaction and the number of the trated animals
- Reading:
- 1st reading
- Hours after challenge:
- 72
- Group:
- test chemical
- Dose level:
- 0.05 ml
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Clinical observations:
- No skin sensitization was observed in treated group.
- Remarks on result:
- no indication of skin sensitisation
- Cellular proliferation data / Observations:
- 1.No skin sensitization was observed in treated group.
2. - Interpretation of results:
- other: not sensitising
- Conclusions:
- The test chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) was considered to be not sensitizing to the skin of guinea pigs.
- Executive summary:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the skin sensitization potential of the test chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1). The studies are as mentioned below:
The peer reviewed journal conductedskin sensitization study for read across substance in 10 female guinea pigs by using modified FCA and guinea pig maximization test.In the induction phase, the test compound was dissolved in FCA, 15 mg in 4 ml. Then physiological saline was added and an emulsion was prepared by mixing the material by mean of a 5ml syringe, until emulsification was completed.The intradermal injections of 6 X 0.10-0.15 ml of this emulsion was applied in a semi-circle arc on clipped and shaved shoulder from left to right in such a way that whole quantity of emulsion was used up for 10 animals. This procedure was repeated on 5thand 9thday. , leaving a gap of 2-3 cm between the rows of injection.After induction phase of 11 days, animals were challenged by applying 0.05ml of test chemical in a subirritant concentration, with further dilution to the clipped and shaved skin of the right flank of the treated animals, allowing 5 mins for drying. Control animals were also used.Mean Observations were made after 24, 48 and 72 hours. As no sensitization effects were observed, the test material can be concluded as non-sensitizing to the female guinea pigs.
The above result was further supported by the skin sensitization study reported by safety assessment reportfor another read across chemicalin guinea pigs. In this study, the test animals were treated dermally with 10% solution of test chemical during induction. After induction, treated animals were dermally exposed to challenge concentration of 1 or 2% solutions of test chemical. Since the test animals failed to induce any cutaneous reaction at challenge concentrations, the test chemical was considered as not sensitizing to guinea pigs.
Based on the above summarized studies for target chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) and its structurally and functionally similar read across substances,it can be concluded that the testchemical is unable to cause skin sensitization and considered as non-skin sensitizer. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.
Reference
1.
Sensitizer |
Challenged with |
Concentration |
24 hour |
48 hour |
72 hour |
24 hour |
48 hour |
72 hour |
||||||||||||||||||||||||||||||
|
|
|
|
|
|
0 |
0 |
0 |
2. Not specified
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
- Additional information:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the skin sensitization potential of the test chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1). The studies are as mentioned below:
The peer reviewed journal conductedskin sensitization study for read across substance in 10 female guinea pigs by using modified FCA and guinea pig maximization test.In the induction phase, the test compound was dissolved in FCA, 15 mg in 4 ml. Then physiological saline was added and an emulsion was prepared by mixing the material by mean of a 5ml syringe, until emulsification was completed.The intradermal injections of 6 X 0.10-0.15 ml of this emulsion was applied in a semi-circle arc on clipped and shaved shoulder from left to right in such a way that whole quantity of emulsion was used up for 10 animals. This procedure was repeated on 5thand 9thday. , leaving a gap of 2-3 cm between the rows of injection.After induction phase of 11 days, animals were challenged by applying 0.05ml of test chemical in a subirritant concentration, with further dilution to the clipped and shaved skin of the right flank of the treated animals, allowing 5 mins for drying. Control animals were also used.Mean Observations were made after 24, 48 and 72 hours. As no sensitization effects were observed, the test material can be concluded as non-sensitizing to the female guinea pigs.
The above result was further supported by the skin sensitization study reported by safety assessment reportfor another read across chemicalin guinea pigs. In this study, the test animals were treated dermally with 10% solution of test chemical during induction. After induction, treated animals were dermally exposed to challenge concentration of 1 or 2% solutions of test chemical. Since the test animals failed to induce any cutaneous reaction at challenge concentrations, the test chemical was considered as not sensitizing to guinea pigs.
Based on the above summarized studies for target chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) and its structurally and functionally similar read across substances,it can be concluded that the testchemical is unable to cause skin sensitization and considered as non-skin sensitizer. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.
Respiratory sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Justification for classification or non-classification
The skin sensitization potential of test substance 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) and its structurally and functionally similar read across substanceswere observed in various studies. From the results obtained from these studies it is concluded that the chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) is not likely to cause skin sensitization and hence can be classified as non-skin sensitizer.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.