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EC number: 701-039-2 | CAS number: 156324-78-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-05-10 to 1994-05-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- (1984)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- signed 1994-03-16
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Samples were taken of fresh and the 48-h old media at test end
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct dispersion in water
- Controls: yes, one control - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna Straus
- Source: laboratory culture
- Age of parental stock: gravid adults
- Feeding during test: none
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Adults were isolated 24 hours prior to test initiation and the overnight produced young daphnids were used for testing. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 270 mg/L as CaCO3
- Test temperature:
- 21.0 °C
- pH:
- 7.6 - 7.7
- Dissolved oxygen:
- at test start 8.4 mg/L
at test end 7.9 - 8.0 mg/L - Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56, and 100 mg test item/L
For measured concentrations please refer to "any other information on results incl. tables", since not all nominal concentrations were analytically verified at 0 hours. - Details on test conditions:
- TEST SYSTEM
- Test vessel:glass jars containing 250 mL test solution
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 25 mL per organism
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water (ISO) according to OECD 202
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: only if necessary to 7.8 ± 0.2
- Photoperiod: 16 h light and 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobilisation
RANGE-FINDING STUDY
- Test concentrations: 0.1, 1.0, 10, and 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 42 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 %-CI: 36 - 51 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- Analysis of the immobility data by the moving average method of Thompson (1947).
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item was found to immobilise Daphnia magna at concentrations higher than 18 mg/L after 48 hours. Based on the nominal test item concentrations, the 48-hour EC50 for Daphnia magna amounts to 42 mg/L (95 %-CI: 36 - 51 mg/L).
- Executive summary:
The toxic effect of the test item to Daphnia magna was examined according to OECD 202 (1984) under static conditions over a period of 48 hours. For the determination of the test concentrations, a range-finding test was performed. In the definitive test, each test concentration and the control was tested in duplicate with ten daphnids each. Effect concentrations were calculated based on nominal test concentration since analytical recoveries were in the range of 80 to 120 % throughout the test. After 48 hours of exposure the test item was found to immobilise Daphnia magna at concentrations above 18 mg/L (NOEC). The corresponding 48-hour EC50 value was estimated with 42 mg/L (95 %-CI: 36 - 51 mg/L).
Reference
Cumulative Immobilisation
Nominal Concentration (mg/L) |
Cumulative immobilised Daphnia (initial population: 10 per replicate) |
|||||||
24 hours |
48 hours |
|||||||
R1 |
R2 |
Total |
% |
R1 |
R2 |
Total |
% |
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1.0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1.8 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3.2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
5.6 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
18 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
32 |
0 |
0 |
0 |
0 |
4 |
3 |
7 |
35 |
56 |
4 |
3 |
7 |
35 |
7 |
6 |
13 |
65 |
100 |
10 |
10 |
20 |
100 |
10 |
10 |
20 |
100 |
R1and R2= Replicates 1 and 2
Analytical results for the test item
Samples |
Nominal concentration (mg/L) |
Concentration found (mg/L) |
Concentration expressed as % of nominal |
0 hours |
Control 1.0 3.2 10 32 100 |
<L.O.D. 0.938 2.88 8.94 28.3 87.5 |
- 93.8 90.0 89.4 88.6 87.5 |
48 hours |
Control 1.0 1.8 3.2 5.6 10 18 32 56 100 |
<L.O.D. 1.01 1.73 3.19 4.72 9.83 16.5 25.3 46.1 84.9 |
- 100.6 95.6 99.6 84.3 98.3 91.6 79.0 82.3 84.9 |
L.O.D. = Limit of detection
Description of key information
The test item was found to immobilise Daphnia magna at concentrations above 18 mg/L (NOEC). The 48-hour EC50 value was estimated with 42 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 42 mg/L
Additional information
The toxic effect of the test item to Daphnia magna was examined according to OECD 202 (1984) under static conditions over a period of 48 hours. For the determination of the test concentrations, a range-finding test was performed. In the definitive test, each test concentration and the control was tested in duplicate with ten daphnids each. Effect concentrations were calculated based on nominal test concentration since analytical recoveries were in the range of 80 to 120 % throughout the test. After 48 hours of exposure the test item was found to immobilise Daphnia magna at concentrations above 18 mg/L (NOEC). The corresponding 48-hour EC50 value was estimated with 42 mg/L (95 %-CI: 36 - 51 mg/L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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