N,N-diethyl-2-propynylamine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not specified

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

no

Test type:

traditional method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

N,N-diethyl-2-propynylamine

EC Number:

223-804-5

EC Name:

N,N-diethyl-2-propynylamine

Cas Number:

4079-68-9

Molecular formula:

C7H13N

IUPAC Name:

diethyl(prop-2-yn-1-yl)amine

Specific details on test material used for the study:

- Name as used in study report: Golpanol DEP; Diethylaminopropin
- Physical: clear yellowish liquid
- Purity: 99%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

- Source: Mura: SPRA (SPF 68 Han), Firma MUS RATTUS, Brunnthal,
- Body weight range: 185 ± 15 g
- The animals were offered Herilan MRH of H. EGGERSMANN KG, Rinteln/Weser, and tap water ad libitum during the post-exposure observation period.
- The animals were kept in fully air-conditioned rooms (temperature 22 ± 2°C and humidity 55 ± 5%) with a day/night rhythm of 12 hours.

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

clean air

Details on inhalation exposure:

- A concentration estimated on the basis of the data from the inhalation hazard test was used as a first orienting concentration. A concentration allows a no effect level to be established (0 lethality).
- Dynamic inhalation system. Whole-body inhalation system (groups of 5 animals are placed in wire cages which are located in a glass-steel inhalation chamber, V = 200 L)
- Generator system: Continuous infusion pump UNITA I; glass evaporator with thermostat
- By means of a continuous infusion pump, constant amounts of the test substance were supplied to an evaporator heated to 49°C. The vapors that were formed were mixed with a flow of fresh air and passed into the inhalation chamber.
- By means of an exhaust air system, the pressure ratios in the inhalation system were adjusted in such a way that there was a pressure slightly below the atmospheric pressure (negative pressure) .

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

- Analytical concentrations: 0.60, 1.54, 1.86, 2.64, 5.95 mg/L
- Nominal concentrations: 1.60, 4.02, 5.35, 8.03, 9.64 mg/L

No. of animals per sex per dose:

10

Control animals:

yes

Details on study design:

- After the exposure period, the surviving animals were observed for 14 days.
- The body weight of the animals was checked before the beginning of the test, after 7 days and at the end of the observation period. The absolute body
weight gain and the relative body weight gain (difference in weight between the initial weight of the animals and the following weights) were determined from the values and presented graphically.
- Clinical findings and lethality were recorded daily.
- At the end of the 14-day observation period, the animals were sacrificed with C02 and were subjected to a gross-pathological examination like all other animals which had died before.

Statistics:

The statistical evaluation of the experiment was based on a probit analysis by D.J. Finney (D. J. Finney; Probitanalysis 1971, page 1 - 150, publisher: Syndics of the Cambridge University Press, Bentley House, 200 Euston Road, London N.W. 1.)

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LC50

Effect level:

2.1 mg/L air

Exp. duration:

4 h

Mortality:

- 0.6 (1.6) mg/L and 1.54 (4.02) mg/L: no deaths;
- 1.86 (5.35) mg/L: 5/10 male and 5/10 females dead animals
- 2.64 (8.03) mg/L: 7/10 male and 8/10 females dead animals
- 9.64 (5.95) mg/L: 10/10 male and 10/10 female dead animals

Clinical signs:

other: - All dose groups: watery eye/nasal secretion (partly reddish), eyelid closure, salivation, intermittent breathing, crouching position, abdominal position, twitching, trembling rsp. clonic convulsions, apathy, staggering gait, ruffled fur. - All symptoms

Body weight:

- Males showed no abnormalities in the body weight growth (compared with the control group).
- Female animals of the test group 2 (2.64 resp. 8.03 mg/L) showed a reduced body weight gain.

Gross pathology:

Animals that died:
- Heart: acute dilatation of the atrium;
- Acute congestive hyperemia;
- Lung: intense blood filling with slight edema;
- Liver: broadened, loam-brown liver lobe periphery; partly liver with lateral corrugation.

Sacrificed animals:
- Nothing abnormal detected.

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria