2-anilinoethanol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed according to OECD guidance and in compliance with GLP criteria.

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Qualifier:

according to guideline

Guideline:

EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

GLP compliance:

yes (incl. QA statement)

Remarks:

BASF Aktiengesellschaft, Experimental Toxicology and Ecology, 67056 Ludwigshafen/Rhein, Germany

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Activated sludge from Iaboratory wastewater plant treating municipal sewage. The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated during the night. 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test temperature:

20 ± 2°C

pH:

- Blanc control and test substance: 7.2 - 7.4
- Reference substance: 7.2 - 7.6

Dissolved oxygen:

- During aeration: >2.5 mg/L
- Immediately before measurement: >6.5 mg/L

Nominal and measured concentrations:

- Nominal: 9.6, 100, 496 and 1000 mg/L

Details on test conditions:

- Test vessels: Erlenmeyer-vessel (nominal volume 250 mL)
- Test volume: 250 mL
- Synthetic medium: 8 mL/vessel 100-fold concentrated OECD medium
- Duration of the measurement of oxygen consumption: 8 - 10 min
- pH correction: yes

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

ca. 15 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

- EC20: ca. 2.7 mg/L
- EC50: ca. 12 mg/L

Oxygen consumption of blank control (BC), mean value of blank control (mv BC) test substance (TS) and reference substance after 30 minutes incubation time

Vessel No.:	BC1	BC2	BC3	Mw BC	TS1	TS2	TS3	TS4	RS1	RS2	RS3
Concentration of test substance (mg/L)	-	-	-	-	1000	496	100	9.6	1	10	100
Added volume of stock solution (mL/vessel)	-	-	-	-	125	62	12.5	1.2	0.5	5.0	50
Direct addition (mg/vessel)	-	-	-	-
Synthetic medium (mL/vessel)	8	8	8	-	8	8	8	8	8	8	8
Inoculum (mL/vessel)	50	50	50	-	50	50	50	50	50	50	50
filled up to the total volume with deionized water (mL/vessel)	250	250	250	-	250	250	250	250	250	250	250
Oxygen concentration start (Mg O2/L)	6.8	7.6	7.1	-	6.7	6.8	6.6	6.7	7.8	8.4	8.4
Oxygen concentration after 6 min (Mg O2/L)	4.0	4.9	4.5	-	5.0	5.0	4.6	4.5	5.1	7.0	8.0
Oxygen consumption rate (Mg O2/L*6 min)	2.8	2.7	2.6	2.7	1.7	1.8	2.0	2.2	2.7	1.4	0.4
Oxygen consumption rate (Mg O2/I*h)	28	27	26	27	17	18	20	22	27	14	4
Specific oxygen consumption rate (mg O2/g*h)	28	27	26	27	17	18	20	22	27	14	4
Change in oxygen consumption rate compared with the blank control (%)	-	-	-	-	37	33	26	19	0	48	85

Reference 2

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Company internal study with unknown GLP status. Limitations in design and reporting but results considered reliable and adequate for assessment.

Qualifier:

no guideline followed

GLP compliance:

no

Analytical monitoring:

no

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Strain: DSM 50026
- Medium: AK-medium DIN 38412 part 8 design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

17 h

Test temperature:

20°C

Nominal and measured concentrations:

- Nominal: 156.25, 312.5, 625, 1250, 2500, 5000, 7500 and 10.000 mg/L

Reference substance (positive control):

no

Duration:

17 h

Dose descriptor:

EC10

Effect conc.:

703.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Duration:

17 h

Dose descriptor:

EC50

Effect conc.:

1 660.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Description of key information

Depending on local conditions and existing concentrations, disturbances in the biodegradation process of activated sludge are possible.

Additional information

The toxicity of 2 -aminoethanol for microorganisms was investigated in two studies. First, the key study, was run according to OECD 209 under GLP, is dated 16 Jul 2003 and identified an EC20 of approx. 15 mg/L (BASF SE, study number 02/0635/08/2). Secound, the supporting study was done as a growth inhibition test for Pseudomonas putida in 1988, non-GLP (BASF SE; Study number 9/0846/88). The study identified an EC10 at 703.4 mg/L after 17h of incubation.