4-tert-butyltoluene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study, a solubiliser was used.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

Cited as Directive 84/449/EEC, C.1 ("Acute toxicity for fish")

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-tert-butyltoluene

EC Number:

202-675-9

EC Name:

4-tert-butyltoluene

Cas Number:

98-51-1

Molecular formula:

C11H16

IUPAC Name:

1-tert-butyl-4-methylbenzene

Details on test material:

- Name of test material (as cited in study report): p-tert-butyltoluene
- Analytical purity: 96.3 %
- Lot/batch No.: none, Reaktor C 510

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1.05 g test substance were mixed with 1 g solubiliser (Marlowet R40) and filled up with 1 l dionised water.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): ricinus oil

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Common carp



ACCLIMATION
- Acclimation period: 14 days
- Type and amount of food: Trouvit, ca 1 % of body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): mortality 7 days before test start <= 5 %

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

12 °dH = ca. 2.14 mmol/l

Test temperature:

20 ± 1°C

pH:

7.8 - 8.2

Dissolved oxygen:

90 - 97 %

Nominal and measured concentrations:

Nominal test concentrations (mg/l)
0.5, 1.0, 2.0, 4.0 and 8.0

Calculated concentrations (based on concentration of test substance in stock solution)
0.32, 0.63, 1.26, 2.52, 5.05 mg/l

Measured test concentrations at test start:
0.56, 0.94, 1.9, 3.9 and 6.1 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: test aquaria (20 l)
- Fill volume: 10 l
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality after 24, 48, 72 and 96 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1.4 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Remarks:

concentrations are given as arithm. means of calculated test concentrations and measured test concentrations after 24 hours

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Remarks:

concentrations are given as arithm. means of calculated test concentrations and measured test concentrations after 24 hours

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

2.8 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Remarks:

concentrations are given as arithm. means of calculated test concentrations and measured test concentrations after 24 hours

Basis for effect:

mortality (fish)

Details on results:

The test substance was not stable in the test medium. The substance was not detectable after 24 hours.