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EC number: 944-041-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- other: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Source study has reliability 2. Details on the read across are available in section 13.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: an aqueous solution (1% w/v) of substance was added to test medium. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium GmbH, Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 2.8 cm (range: 2.4 - 3.4 cm)
- Weight at study initiation (mean and range, SD): 0.26 g
ACCLIMATION
- Type and amount of food: TETRA MIN, standard feed for aquarium fish, Tetra-Werke, Melle, Germany; ad libitum.
- Feeding frequency: daily.
- Health during acclimation (any mortality observed): no mortality observed. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 23 - 25°C
- pH:
- 7.8 - 8.1
- Dissolved oxygen:
- 7.4 - 8 mg/l
- Nominal and measured concentrations:
- Nominal test substance concentrations:
6.81, 10.00, 14.70, 21.50, 31.60, 46.40, 68.10, 100.00 mg/l
Analytically determined concentrations:
5.10, 7.80, 11.40, 16.90, 24.00, 35.70, 51.00 and 76.60 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquarium ( 30 × 22 × 24 cm)
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.26 g fish/l
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412 part 11
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 24, 48, 72 and 96 hours. - Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 20.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 46.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Gasping and tumbling were observed. Since test solutions were intensly coloured, symptoms could not be observed in more detail.
- Reported statistics and error estimates:
- FINNEY, D.J., PROBIT ANALYSIS, CAMBR. UNIV. PRESS, 3RD ED., 1971
- Conclusions:
- LC50 (96h) = 20.7 mg/l.
- Executive summary:
Method:
Acute toxicity to fish was assessed using nominal test concentrations of 6.81, 10.0, 14.7, 21.5, 31.6, 46.4, 68.1, 100 mg/l. Ten fish per concentration were exposed under static conditions for up to 96 h. Effects in terms of mortality were recorded at 1, 24, 48, 72 and 96 h after start.
Results:
LC50 (96h) = 20.7 mg/l
- Endpoint:
- short-term toxicity to fish
- Type of information:
- other: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Source study has reliability 2. Details on the read across are available in section 13.
- Principles of method if other than guideline:
- Test method described in JIS K 0102 - 1974-ITEM 55.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: the substance was added to test medium without any pretreatment. - Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: red Killifish.
- Source: bred at testing facility.
- Length at study initiation (length definition, mean, range and SD): 3.0 cm (range: 2.8 - 3.4 cm).
- Weight at study initiation (mean and range, SD): 0.26 g.
ACCLIMATION
- Type and amount of food: TETRA MIN, standard feed for aquarium fish, Tetra-Werke, Melle, Germany; ad libitum.
- Feeding frequency: daily. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 26°C
- pH:
- 8.2 - 8.6
- Dissolved oxygen:
- 7.3 - 8.1 mg/l
- Nominal and measured concentrations:
- Nominal test substance concentrations:
46.4, 100, 215, 464 and 1000 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquarium ( 30 × 22 × 24 cm)
- Fill volume: 5 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.5 g fish/l
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water, filtered by carbon filter
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptomas of intoxication after 24, 48 and 96 hours.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Results used to determine the conditions for the definitive study: LC50 after 96 h: ca. 250 mg/l - Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 46.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 181 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Symptoms of intoxication were not observed.
- Reported statistics and error estimates:
- FINNEY, D.J., PROBIT ANALYSIS, CAMBR. UNIV. PRESS, 3RD ED., 1971
- Conclusions:
- LC50 (96h) = 181 mg/l
- Executive summary:
Method:
Acute toxicity to fish was assessed using nominal test concentrations of 46.4, 100, 215, 464 and 1000 mg/l. Ten fish per concentration were exposed under static conditions for up to 96 h. Effects in terms of mortality were recorded at 0, 24, 48 and 96 h after start.
Results:
LC50 (96h) = 181 mg/l.
Referenceopen allclose all
Description of key information
LC50 (96h) freshwater, Zebra fish = 20.7 mg/l
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 20.7 mg/L
Additional information
No data on target substance was available, thus a read across approach was followed, using data on Similar Substance 01. Details are reported in section 13.
Short term toxicity to fish was assessed in 2 studies on Similar Substance 01, under static exposure conditions and exposure duration of 96 h, using 2 different species. Both studies were well-documented and reliable, thus the study reporting the lowest LC50 value was used for classification, following a precautionary approach.
In a study conducted according to OECD guideline 203 on Danio rerio species, nominal tested concentrations ranged from 6.81 to 100 mg/l. One replicate with 10 fish per concentration was tested. Effects of mortality were recorded at 1, 24, 48, 72 and 96 h after start of exposure. LC50(96h) of 20.7 mg/l was found.
In a study conducted on the species Oryziaz latipes, concentrations in the range of 46.1 up to 1000 mg/l were used. After 96 h, a LC50 of 181 mg/l was found.
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