Sodium cumenesulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

GLP compliance:

yes

Specific details on test material used for the study:

CAS Number: 28348-53-0
Identity: Cumene sulfonic acid, sodium salt
Purity: 45%
Remarks: Substances tested are aqueous solutions; % purity
equates to chemical content

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Concentrations: Nominal: 100, 180, 320, 560 and 1000 mg/L (no vehicle);
untreated controls. Nominal concentrations have not been corrected for
sample purity.
Test conditions: Static without aeration; at 20 ± 2 °C in 250 mL glass vessels
containing 200 mL of medium of hardness 146 mg/L (CaCO3) and pH 8.2;
16 hours light, unfed.
Physical measurements: At 0 and 48 hours: overall ranges for pH 8.2-8.4; O2
86-89%; temperature 20-21 °C.
Observations: Immobility/symptoms at 24 and 48 hours.

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magna, <24 hours old
Number of daphnids: 10/beaker, 2 beakers/treatment.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

146 mg/L (CaCO3

Test temperature:

20 ± 2 °C

pH:

8.2

Dissolved oxygen:

O2
86-89%

Salinity:

NR

Nominal and measured concentrations:

Concentrations: Nominal: 100, 180, 320, 560 and 1000 mg/L (no vehicle);
untreated controls. Nominal concentrations have not been corrected for sample purity

Details on test conditions:

Test conditions: Static without aeration; at 20 ± 2 °C in 250 mL glass vessels containing 200 mL of medium of hardness 146 mg/L (CaCO3) and pH 8.2;
16 hours light, unfed.
Physical measurements: At 0 and 48 hours: overall ranges for pH 8.2-8.4; O2 86-89%; temperature 20-21 °C.
Observations: Immobility/symptoms at 24 and 48 hours.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 48-h EC50 >1000 mg/L (equivalent to 450 mg/L active ingredient considering the 45% sample purity).

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 450 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Remarks on result:

other: 48-h EC50 >1000 mg/L (equivalent to 450 mg/L active ingredient considering the 45% sample purity).

Details on results:

A 48 h EC50 > 1000 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna (48-h EC50 >1000 mg/L (equivalent to 450 mg/L active ingredient considering the 45% sample purity)

Results

Unit: mg/L

		Nominal Concentration (mg/L)
Parameter	Time [hours]	0	100	180	320	560	1000
Immobility [%]	48	0	0	0	0	5	0
Symptoms	48	No treatment related effects

Validity criteria fulfilled:

yes

Conclusions:

A 48 h EC50 > 1000 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna (48-h EC50 >1000 mg/L (equivalent to 450 mg/L active ingredient considering the 45% sample purity).
The test material is not acutely toxic to Daphnia magna at up to 1000 mg/L, corresponding to 450 mg/L active ingredient

Executive summary:

A 48 h EC50 > 1000 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna (48-h EC50 >1000 mg/L (equivalent to 450 mg/L active ingredient considering the 45% sample purity).

The test material is not acutely toxic to Daphnia magna at up to 1000 mg/L, corresponding to 450 mg/L active ingredient

Description of key information

A 48 h EC50 > 1000 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna (48-h EC50 >1000 mg/L (equivalent to 450 mg/L active ingredient considering the 45% sample purity).

The test material is not acutely toxic to Daphnia magna at up to 1000 mg/L, corresponding to 450 mg/L active ingredient

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

450 mg/L

Marine water invertebrates

Marine water invertebrates

Effect concentration:

45 mg/L

Additional information

The available data for saltwater invertebrates a are limited and to obtaine the value for PNECs for saltwaters is taken account a assesment factor 10 to extrapolate to NOEC following the “Guidance on Information Requirement and Chemical Safety Assessment R.10: Characterisation of dose [concentration]- response for environment”and “Table R.10-5 Assessment factors proposed for deriving PNEC water for saltwater for different data sets”, to account for the possibility that greater taxonomic diversity in the marine environment makes it likely that some marine taxa may be more sensitive than the most sensitive freshwater taxa