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EC number: 300-346-5 | CAS number: 93925-43-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 - 16 Feb 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- March 2006
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- July 2009
- Qualifier:
- according to guideline
- Guideline:
- ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
- Version / remarks:
- October 2004
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Food and Consumer Product Safety Authority (VWA), Utrecht, The Netherlands
- Analytical monitoring:
- no
- Remarks:
- No suitable analytical method could be established to quantify the test substance in test medium.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Preparation of test solutions started with individually prepared loading rates of 1.0, 10 and 100 mg/L applying a 1 h period of magnetic stirring. The obtained mixtures containing undissolved material were allowed to settle for a period of 1 h after which the Water Accommodated Fractions were siphoned off and used as test solutions. The lowest test concentration was prepared as a 10-fold dilution of the WAF prepared at 1.0 mg/L. After preparation, volumes of 50 mL were added to each replicate of the respective test concentration. Subsequently, 1 mL of an algal suspension was added to each replicate providing a cell density of 10E+04 cells/mL.
- Eluate: no
- Differential loading: yes, except for the lowest test concentration
- Controls: yes, test medium control
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): The final test solutions were all clear and colourless. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: NIVA CHL 1
- Source (laboratory, culture collection): laboratory culture
- Method of cultivation: Algae stock cultures were started by inoculating growth medium with algal cells from a pure culture on agar. The suspensions were continuously aerated and exposed to light in a climate room at a temperature of 21-24 °C. The stock culture medium was M1 medium according to NPR 6505 formulated in Milli-RO water. 3 d before the start of the test, cells from the algal stock culture were inoculated in culture medium at a cell density of 1 x 10E+04 cells/mL. The pre-culture was maintained under the same conditions as used in the test. The cell density was measured immediately before use. The medium for the pre-culture was M2; according to the OECD 201 Guideline, formulated using Milli-Q water (tap water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ion-exchange cartridges. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- 24 mg CaCO3/L
- Test temperature:
- 21.5 - 22.2 °C
- pH:
- 8.0 - 8.2
- Nominal and measured concentrations:
- nominal: control, 0.1, 1.0, 10, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: all-glass test vessels
- Material, size, headspace, fill volume: all-glass, 100 mL, headspace: 50 mL, fill volume: 50 mL
- Aeration: continuous shaken
- Initial cells density: 1.00E+04 cells/mL
- Control end cells density: 79.5 - 134E+04 cells/mL
- No. of vessels per concentration (replicates): 3 (0.1, 1.0 and 10 mg/L), (100 mg/L)
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes, according to guideline
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M2; according to the OECD 201 Guideline, formulated using Milli-Q water (tap water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ion-exchange cartridges preventing precipitation.
- Culture medium different from test medium: M1 medium was used for stock culture, M2 medium was used for pre-culture
- Intervals of water quality measurement: The pH was measured at the beginning and end of the test in the control and the highest test concentration. Temperature was continuously monitored in a temperature control vessel.
OTHER TEST CONDITIONS
- Photoperiod: continuous illumination
- Light intensity and quality: TLD-lamps of the type ‘Cool-white’ of 30 W, with a light intensity within the range of 75 to 87 µE*m-2*s-1.
EFFECT PARAMETERS MEASURED
- Determination of cell concentrations: Algal density was determined by use of a microscope and a counting chamber throughout the test.
- Appearance: At the end of the test microscopic observations were performed on the limit concentration to observe for any abnormal appearance of the algae.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- other: yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- other: yield
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): Microscopic observations at the end of the test revealed a normal and healthy appearance of the exposed cells when compared to the control.
- Any stimulation of growth found in any treatment: yes, stimulation of growth compared to the control was recorded in each tested loading rate.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: In the three highest test groups a floating layer was observed at 24 and 48 h. At the end of the test small particles of the test substance were observed in the highest concentration. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: ErC50 (72 h) 1.8 mg/L (95% CI: 1.4 - 2.4 mg/L), EyC50 (72 h) 0.69 mg/L (95% CI 0.50 - 0.95 mg/L)
- Other: The historical ranges for growth rate reduction lies between 0.82 and 2.3 mg/L. The historical ranges of the EC50 (72 h) for yield inhibition lie between 0.43 and 1.1 mg/L. - Reported statistics and error estimates:
- Statistical analysis of the data was not needed as the effects recorded were not significant (<10%). Stimulation of growth was recorded.
- Validity criteria fulfilled:
- yes
Reference
Table 1: Growth rate and reduction of growth rate during exposure.
Loading rate [mg/L] |
Test vessel |
Growth rate [µ] |
Growth rate reduction [%] |
||||
0-24 h |
24-48 h |
48-72 h |
0-24 h |
24-48 h |
48-72 h |
||
control |
1 |
0.07103 |
0.06512 |
0.04617 |
|
|
|
2 |
0.08108 |
0.05701 |
0.05870 |
||||
3 |
0.07956 |
0.04778 |
0.06060 |
||||
4 |
0.07466 |
0.06636 |
0.06306 |
||||
5 |
0.07636 |
0.04407 |
0.06541 |
||||
6 |
0.08395 |
0.05099 |
0.05458 |
||||
Mean |
0.07777 |
0.05522 |
0.05809 |
||||
CV |
6% |
17% |
12% |
||||
10% of 1.0 |
1 |
0.06706 |
0.06622 |
0.06568 |
14 |
-20 |
-13 |
2 |
0.06909 |
0.06862 |
0.05479 |
11 |
-24 |
6 |
|
3 |
0.06029 |
0.07036 |
0.06558 |
22 |
-27 |
-13 |
|
1.0 |
1 |
0.05776 |
0.08108 |
0.06064 |
26 |
-47 |
-4 |
2 |
0.06029 |
0.07818 |
0.06136 |
22 |
-42 |
-6 |
|
3 |
0.07636 |
0.06395 |
0.05517 |
2 |
-16 |
5 |
|
10 |
1 |
0.07103 |
0.06927 |
0.05281 |
9 |
-25 |
9 |
2 |
0.07288 |
0.06596 |
0.06267 |
6 |
-19 |
-8 |
|
3 |
0.07103 |
0.06855 |
0.06059 |
9 |
-24 |
-4 |
|
100 |
1 |
0.06909 |
0.07049 |
0.06373 |
11 |
-28 |
-10 |
2 |
0.06267 |
0.07388 |
0.06594 |
19 |
-34 |
-14 |
|
3 |
0.06267 |
0.07103 |
0.06863 |
19 |
-29 |
-18 |
|
4 |
0.07288 |
0.06952 |
0.05725 |
6 |
-26 |
1 |
|
5 |
0.07103 |
0.06391 |
0.06383 |
9 |
-16 |
-10 |
|
6 |
0.07636 |
0.04949 |
0.07131 |
2 |
10 |
-23 |
Analytical measurements
The test substance is an organic compound with the element tin (Sn) in its molecular structure. An ICP-MS method was developed for the determination of the test substance based on the element Sn. Validation of the analytical assay was carried out, however, it was found that the accuracies on test samples prepared with the test substance were inadequate. The ICP-MS method developed was considered to be the best possible for the determination of the test substance:
- Oxygen and helium gas were used to remove the organic and tetrahydrofuran (THF) content of the test substance and accuracy samples.
- The analytical sequence had a normal number of samples and the instrument was cleaned prior to use.
- The substance must be dissolved in THF since it was not directly soluble in (aqueous) strong acids such as HNO3.
According to the results obtained it was concluded that it is not possible to use the ICP-MS method for quantification of test samples to support physico-chemical- and (eco)toxicologal studies. Other analytical techniques such as GC, HPLC and UV-Vis were not suitable for the determination of the test substance due to its hydrophobic molecular structure.
Description of key information
ErL50 (72 h) > 100 mg/L (nominal, OECD 201)
NOErLR (72 h) ≥ 100 mg/L (nominal, OECD 201)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
One experimental study is available investigating the effects of the substance to freshwater algae.The study was performed according to OECD 201 (GLP) with Pseudokirchneriella subcapitata under static conditions. Test solutions were prepared as loading rates of 0.1, 1.0, 10.0 and 100 mg/L by adding appropriate amounts of the substance to test medium followed by 1 h stirring 1 h settling and siphoning off the soluble fraction to be used for testing. Only the lowest loading rate of 0.1 mg/L was prepared by serial dilution (10x) of 1 mg/L. All test solutions were clear and colorless throughout the test. Analytical measurements were not possible to be conducted since no suitable analytical method could be established (see also IUCLID section 5.1.2). No inhibition of growth was recorded after 72 h resulting in an ErL50 (72 h) of > 100 mg/L and a NOErLR (72 h) ≥ 100 mg/L.
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