Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 226-841-5 | CAS number: 5502-88-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Screening Test
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Version / remarks:
- Guideline currently in draft with the OECD
- Principles of method if other than guideline:
- An equal volume of specialized growth medium containing BlueScreen HC cells was added to each well.
Microplates were covered with a breathable membrane and incubated at 37°C, 5% CO2 and 95% humidity. After 3 hours, cells were
washed with phosphate buffered saline, centrifuged, and placed in recovery medium. After an additional 45 hours of incubation, the
fluorescence and flash luminescence of each well was measured using a microplate reader. Cytotoxicity was measured by the lysis of
cells followed by staining with a fluorescent DNA binding stain to provide an estimate of relative cell density. - GLP compliance:
- yes
- Type of assay:
- other: (GLuc-T01), which contain a Gaussia luciferase (GLuc)-based reporter system
Test material
- Reference substance name:
- 4-isopropyl-1-methylcyclohexene
- EC Number:
- 226-841-5
- EC Name:
- 4-isopropyl-1-methylcyclohexene
- Cas Number:
- 5502-88-5
- Molecular formula:
- C10H18
- IUPAC Name:
- 1-methyl-4-(propan-2-yl)cyclohex-1-ene
- Reference substance name:
- rel-(1R,4R)-1-isopropyl-4-methylcyclohexane
- Cas Number:
- 1678-82-6
- Molecular formula:
- C10H20
- IUPAC Name:
- rel-(1R,4R)-1-isopropyl-4-methylcyclohexane
- Reference substance name:
- rel-(1S,4S)-1-isopropyl-4-methylcyclohexane
- Cas Number:
- 6069-98-3
- Molecular formula:
- C10H20
- IUPAC Name:
- rel-(1S,4S)-1-isopropyl-4-methylcyclohexane
- Test material form:
- liquid
Constituent 1
impurity 1
impurity 2
- Specific details on test material used for the study:
- Colourless liquid
Method
- Target gene:
- (GLuc-T01), which contain a Gaussia luciferase (GLuc)-based reporter system
Species / strain
- Species / strain / cell type:
- human lymphoblastoid cells (TK6)
- Details on mammalian cell type (if applicable):
- TK6 cells (GLuc-T01), which contain a Gaussia luciferase
(GLuc)-based reporter system. - Additional strain / cell type characteristics:
- not applicable
- Cytokinesis block (if used):
- No
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rats
- Test concentrations with justification for top dose:
- 2097.15 to 10000 μM based on cytoxicity
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- cyclophosphamide
- Evaluation criteria:
- Luminescence induction below threshold of 1.8.
Microsoft Excel was used to store raw data that were analyzed by BlueScreen HC software to produce a semi-quantitative assessment of cytotoxicity and genotoxicity.
Results and discussion
Test results
- Key result
- Species / strain:
- human lymphoblastoid cells (TK6)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- In the absence of S9:
Dose: 10000 micromolar Effects: Cytotoxicity
Results: Luminescence induction below threshold of 1.8; value=0.0
In the presence of S9:
Dose: 10000 micromolar Effects: Cytotoxicity
Results: Luminescence induction below threshold of 1.5; value=0.0. - Remarks on result:
- other: Negative
Applicant's summary and conclusion
- Conclusions:
- Absence of S9: For this protocol, the statistically defined threshold for a positive genotoxic result is 1.8 (i.e., 80% induction over and above the baseline for vehicle controls). The positive control passed the test criteria.
Presence of S9: For this protocol, the statistically defined threshold for a positive genotoxic result is 1.5 (i.e., 50% induction over and above the baseline for vehicle controls). The positive control passed the test criteria. - Executive summary:
Negative for genotoxicity in the absence and presence of S9.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.