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EC number: 226-841-5 | CAS number: 5502-88-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- The study was conducted between 19 August 2017 and 27 September 2017 (biological phase).
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 4-isopropyl-1-methylcyclohexene
- EC Number:
- 226-841-5
- EC Name:
- 4-isopropyl-1-methylcyclohexene
- Cas Number:
- 5502-88-5
- Molecular formula:
- C10H18
- IUPAC Name:
- 1-methyl-4-(propan-2-yl)cyclohex-1-ene
- Reference substance name:
- rel-(1R,4R)-1-isopropyl-4-methylcyclohexane
- Cas Number:
- 1678-82-6
- Molecular formula:
- C10H20
- IUPAC Name:
- rel-(1R,4R)-1-isopropyl-4-methylcyclohexane
- Reference substance name:
- rel-(1S,4S)-1-isopropyl-4-methylcyclohexane
- Cas Number:
- 6069-98-3
- Molecular formula:
- C10H20
- IUPAC Name:
- rel-(1S,4S)-1-isopropyl-4-methylcyclohexane
- Test material form:
- liquid
Constituent 1
impurity 1
impurity 2
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Details on test solutions:
- Frequency and Duration of Administration, Treatment Levels, and Replication
Based on the results of a preliminary non-GLP range finding test, the following concentrations in a geometrical series (spacing factor: 2.2) were tested in the definitive test (DT2):
4.27, 9.39, 20.7, 45.5 and 100% of a saturated solution at a loading rate of 200 mg/L.
The test period (exposure of test organisms to the test solutions in a semi-static system) was 48 hours.
Additionally, daphnids were exposed under control conditions (non-treated test medium without solvent).
Four replicates were used for the test item concentrations and the control. Each replicate vessel contained five daphnids.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test System
• Species: The test system used in this study was Daphnia magna STRAUS (clone M10), a water flea, cultured at ECT Oekotoxikologie GmbH since December 22, 2011.
• Origin: The organisms were originally supplied by KU Leuven, Belgium.
The health of the Daphnia culture and the overall quality of the test organisms is monitored regularly by using the reference toxicant potassium dichromate (K2Cr2O7) in an acute test design in order to determine the sensitivity of the organisms from the culture and to demonstrate that the test conditions are appropriate.
The most recent reference test with potassium dichromate performed in a separate study (Study No. IDA1705) resulted in an EC50(24 h) of 1.29 mg/L.
According to the OECD guideline No. 202 (OECD, April 2004) EC50(24 h)-values for potassium dichromate obtained from different laboratories should range between 0.6 mg/L and 2.1 mg/L. The toxicity of the reference item is within this range. Therefore the results of this reference test are acceptable and the test conditions are reliable and the sensitivity of the test system could be demonstrated.
Study design
- Test type:
- semi-static
- Water media type:
- other: Elendt medium M4 was used to dilute the test item and to keep the daphnids during the period of the test.
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20.2 - 21.2 °C
- pH:
- 7.5 - 7.8
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.292 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI: 0.253 to 0.367 mg/L
- Duration:
- 48 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 0.229 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.195 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.137 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the statistical evaluation of biological results and geometric measured concentrations of the test item, the 48-hour EC50 for Daphnia magna was 0.292 mg/L (95% confidence limits: 0.253 - 0.367 mg/L).
- Executive summary:
The acute toxicity of the test item Menthene,-1, para to Daphnia magna was determined in a 48-hour test according to the OECD Guideline for Testing of Chemicals, No. 202 (2004) and the Commission Regulation (EC) No. 440/2008, Part C.2.
Based on the statistical evaluation of biological results and mean measured concentrations of the test item, the 48-hour EC50 for Daphnia magna was 0.292 mg/L (95% confidence limits: 0.253 - 0.367 mg/L).
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