Propiononitrile

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2nd July 1979- 31st July 1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

2079

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

not specified

GLP compliance:

not specified

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Propiononitrile

EC Number:

203-464-4

EC Name:

Propiononitrile

Cas Number:

107-12-0

Molecular formula:

C3H5N

IUPAC Name:

propanenitrile

Test material form:

liquid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Male rats used in the study with average weight/dose ranging 230-245 g; Female rats used in the study with average weight/dose ranging 235-245 g.

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

unchanged (no vehicle)

Doses:

Administered to male: 50.1, 63.1, 79.4, 100, 126, 158 mg/kg body weight;
Administered to female: 158, 200, 251, 316 mg/kg body weight.

No. of animals per sex per dose:

5 males/dose; and 5 females/dose.

Control animals:

not specified

Statistics:

95% confidence limits stated

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

75 mg/kg bw

Based on:

test mat.

95% CL:

58 - 98

Effect levels 2

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

270 mg/kg bw

Based on:

test mat.

95% CL:

235 - 310

Mortality:

Two out of five males died following 63.1 mg/kg exposure to the test substance; Four out of five males died following 79.4 mg/kg exposure to the test substance; Three out of five males died following 100 mg/kg exposure to the test substance; Three out of five males died following 126 mg/kg exposure to the test substance; Four out of five males died following 158 mg/kg exposure to the test substance.
One out of five females died following 200 mg/kg exposure to the test substance; One out of five females died following 251 mg/kg exposure to the test substance; Four out of five females died following 316 mg/kg exposure to the test substance;

Clinical signs:

other: Signs of intoxication in both sexes included: weight loss, increasing weakness, ocular discharge, tremors, convulsions, collapse and death. In females in addition to already listed signs, diarrhoea and dyspnoea were also observed.

Gross pathology:

In both sexes: haemorrhagic lungs, liver hyperthermia and discolouration in some instances, gastrointestinal inflammation (acute in some instances).

Other findings:

In survivors (14 days), viscera appeared normal in both sexes.

Any other information on results incl. tables

Acute oral toxicity in male rats.

Dose (mg/kg)	Mean initial weight (g)	Mortality out of five/dose
50.1	230	0
63.1	235	2
79.4	245	4
100	240	3
126	240	3
158	235	5

Time of mortality: 1 -2 days post exposure. Slope 3.9

Acute oral toxicity in female rats.

Dose (mg/kg)	Mean initial weight (g)	Mortality out of five/dose
158	245	0
200	245	1
251	245	1
316	235	4

Time of mortality: 1 -3 days post exposure. Slope 9.0

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

The oral LD50 for males was 75 mg/kg bw and for females it was 270 mg/kg bw. The substance is therefore classified as category 3 for acute oral toxicity.

Executive summary:

The substance was used in an acute oral toxicity study using male and female rats (n=5/sex). The male rats were orally exposed to 50.1, 63.1, 79.4, 100, 126, 158 mg/kg body weight of undiluted substance. The female rats were orally exposed to 158, 200, 251, 316 mg/kg body weight of undiluted substance. Two out of five males died following 63.1 mg exposure to the test substance. Four out of five females died following 316 mg/kg exposure to the test substance. The oral LD50 for males was 75 mg/kg bw and for females it was 270 mg/kg bw. The substance is therefore classified as category 3 for acute oral toxicity.