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EC number: 915-634-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 October 2020 - 04 March 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Date of inspection: 20 to 21 November 2018 and 07 June 2019; Date of decision: 21 August 2019
- Analytical monitoring:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture
- Name and location of sewage treatment plant where inoculum was collected:
Activated sludge was obtained from a water treatment plant treating predominantly domestic sewage (Bois-de Bay, 1242 Satigny, Switzerland). The sludge was kept aerobic until being used on the next day - Test type:
- static
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2°C
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500-ml scaled cylinders
- Type: closed
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Sludge concentration (weight of dry solids per volume): 1.5 g/L
- Nutrients provided for bacteria: Synthetic sewage feed
EFFECT PARAMETERS MEASURED: oxygen consumption at 3 hours - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- The EC50 (3 hours) of 3,5-dichlorophenol is between 2 and 25 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the EC50(3 hours) of test item > 100 mg/L and NOEC (3 hours) of test item is 100 mg/L.
- Executive summary:
An activated sludge respiration inhibition test was performed with test item according to OECD Guideline 209, ISO 8192-1986 and EU method C.11, Activated Sludge Respiration Inhibition Test. In a limit test, a concentration of 100 mg/L (nominal) was evaluated.
The percentage values of inhibition in the three test replicates of 11.7%, 11.7% and 9.9% are well within the 30% error (validity criteria) of the test, and therefore we can consider that at the tested concentration (nominal 100 mg/L) no significant inhibition with test item was observed.
Therefore, EC50(3 hours) of test item > 100 mg/L; NOEC (3 hours) of test item is 100 mg/L.
Reference
Table 6.1.7/1 % inhibition by test item
Recipient |
pH |
O2(mg/L) T0 min |
O2(mg/L) T5.0 min |
Δ O2(mg/L) |
Temps (min) |
Rate |
% Inhibition |
Concentration finale (mg/L) |
C1 |
8.41 |
6.80 |
4.30 |
2.50 |
5.0 |
0.50 |
- |
- |
C2 |
8.46 |
6.70 |
4.30 |
2.40 |
5.0 |
0.48 |
- |
- |
C3 |
8.50 |
7.75 |
5.25 |
2.50 |
5.0 |
0.50 |
- |
- |
S1 |
8.40 |
6.80 |
4.35 |
2.45 |
5.0 |
0.49 |
11.7 |
100.16 |
S2 |
8.47 |
7.80 |
5.35 |
2.45 |
5.0 |
0.49 |
11.7 |
99.84 |
S3 |
8.44 |
7.30 |
4.80 |
2.50 |
5.0 |
0.50 |
9.9 |
100.10 |
R1 |
8.45 |
- |
- |
- |
- |
- |
- |
2.14 |
R2 |
8.42 |
6.75 |
4.30 |
2.45 |
5.0 |
0.49 |
11.7 |
2.12 |
R3 |
8.47 |
7.50 |
5.00 |
2.50 |
5.0 |
0.50 |
9.9 |
2.08 |
R4 |
8.51 |
8.30 |
7.40 |
0.90 |
5.0 |
0.18 |
67.6 |
25.14 |
R5 |
8.55 |
8.45 |
7.55 |
0.90 |
5.0 |
0.18 |
67.6 |
24.92 |
R6 |
8.47 |
8.25 |
7.30 |
0.95 |
5.0 |
0.19 |
65.8 |
24.92 |
C4 |
8.51 |
6.65 |
3.70 |
2.95 |
5.0 |
0.59 |
- |
- |
C5 |
8.56 |
8.15 |
5.15 |
3.00 |
5.0 |
0.60 |
- |
- |
C6 |
8.58 |
8.10 |
4.80 |
3.30 |
5.0 |
0.66 |
- |
- |
The data of reference number 1 (R1) is not exploitable due to a measurement error, as Reference 2 (R2) and Reference 3 (R3) are in very good agreement the results of this study are not impacted.
- The variation coefficient of the controls was 13.0%. This is within the validity range (30%).
- the EC50(3 hours) of 3,5-dichlorophenol is between 2 and 25 mg/L.
Description of key information
An activated sludge respiration inhibition test was performed with test item according to OECD Guideline 209, ISO 8192-1986 and EU method C.11, Activated Sludge Respiration Inhibition Test. In a limit test, a concentration of 100 mg/L (nominal) was evaluated. The percentage values of inhibition in the three test replicates of 11.7%, 11.7% and 9.9% are well within the 30% error (validity criteria) of the test, and therefore it can be considered that at the tested concentration (nominal 100 mg/L) no significant inhibition with Ethyl Safranate was observed.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
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