Registration Dossier
Registration Dossier
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EC number: 256-456-8 | CAS number: 49744-25-4
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 Mar - 02 May 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 425-310-7
- EC Name:
- -
- Cas Number:
- 77889-90-8
- Molecular formula:
- C14H10ClN5O4
- IUPAC Name:
- 5-[2-(4-chloro-2-nitrophenyl)diazen-1-yl]-6-hydroxy-1,4-dimethyl-2-oxo-1,2-dihydropyridine-3-carbonitrile
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: WWTP STP Odenthal, Germany, collected on 2006.03-27
- Pretreatment: none
- Concentration of sludge: 30 mg/L ss - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Initial conc.:
- 966 other: mg O2/g
- Based on:
- ThOD
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 20 - 24°C
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Voith Sapromat, 250 mL test volume
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: magnet stirring
SAMPLING
- Sampling frequency: days 2, 6, 8 , 10, 14, 16, 22, 24, 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- St. dev.:
- 0
- Sampling time:
- 28 d
- Details on results:
- Toxicity control: 54% biodegradation on day 14. Test item is not inhibitory to activated sludge respiration.
pH: 7.5 - 7.6
BOD5 / COD results
BOD5 / COD
- Parameter:
- BOD5
- Value:
- 0 mg O2/g test mat.
- Results with reference substance:
- 86% biodegradation on day 14, 91% on day 28; validity criteria fulfilled.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test item is not readily biodegradable (0% after 28 d based on oxygen consumption). The substance is not inhibitory to activated sludge respiration.
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