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EC number: 939-559-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 Feb – 14 Mar 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesanstalt für Umwelt, Messungen und Naturschutz Baden-Würtemberg, Germany
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: To the test mixtures (500 mL), which contained deionised water (234 mL), synthetic sewage feed (16 mL) and the appropriate test / reference item concentration, the inoculum of activated sludge (250 mL) was added. The test item was prepared by directly weighing 50 mg of the test item into 500 mL. All components were dispersed by intense aeration.
A stock solution of 3,5-dichlorophenol was prepared by dissolving 200 mg DCP in 200 mL water. The reference item was dosed by dissolving defined volumes of the stock solution in the test solutions. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: Municipal wastewater treatment plant Pforzheim, Germany, collected from the aeration tank
- Preparation of inoculum for exposure: The sludge was used one day after collection. It was settled and the upper layer with finer solids was decanted and discarded. Before starting the test, the remaining sludge was washed two times with tap water by centrifugation (10 minutes at 3000 rpm). After centrifuging, the supernatant was decanted and discarded and the sludge was resuspended in chlorine free tap water. This procedure was repeated once. The mixed liquor suspended solids (MLSS) in the sludge inoculum were adjusted to a concentration of 3.0 g/L (± 10%). The activated sludge was continuously aerated at the test temperature, the solids did not settle down. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 19.3 – 20.9 °C
- pH:
- 7.21 - 7.25 (control)
6.38 -6.88 (pH of test item concentration and abiotic control) - Nominal and measured concentrations:
- control, 100 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 1 litre glass beakers filled with 500 mL test solution
- Aeration: yes
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per abiotic control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Synthetic medium: The test was performed in a synthetic sewage feed that was prepared with the following amounts of substances in 1 litre of deionised water: 16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCl, 0.4 g CaCl2 × 2 H2O, 0.2 g MgSO4 × 7 H2O, 2.8 g K2HPO4.
The solution was sterilized prior to storage. If the preparation was not used immediately it was stored in the dark at a temperature of between 0 – 10 ºC for 4 days.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Respiration rate measurements were conducted 3 hours after test start
TEST CONCENTRATIONS
- Range finding study
Test concentrations: control, 10.0, 100 and 1000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- The EC50 of the total oxygen uptake for the reference item 3,5-dichlorophenol (DCP) was estimated to be between 5 and 25 mg/L after 3 hours. This is within the recommended range of 2 to 25 mg/L.
- Reported statistics and error estimates:
- The statistical evaluation was performed for specific respiration rate of the control and the test item concentrations using SAS® (2002–2010). The
calculation was performed using SAS Software service pack 9.3. The NOEC was determined using the t-Test pooled. P-values below 0.05 showed statistically significant differences to the control.
Reference
Table: Results of the range-finding test, specific respiration rates
Test assay nominal [mg/L] |
Specific respiration rates after 3 h [mg O2/(g × h)] |
Control (1) |
24.13 |
Control (2) |
24.58 |
Control (3) |
23.93 |
10.0 |
23.71 |
100 |
24.06 |
1000 (1) |
0.88 |
1000 (2) |
0.27 |
1000 (3) |
0.63 |
DCP 0.2 |
23.44 |
DCP 1 |
18.35 |
DCP 5 |
14.94 |
DCP 25 |
3.47 |
Abiotic control |
-0.26 |
Table: Results of the range-finding test, inhibition
Test assay nominal [mg/L] |
Inhibition after 3 h [%] |
10.0 |
2.1 |
100 |
0.6 |
1000 (mean) |
97.6 |
DCP 0.2 |
3.2 |
DCP 1 |
24.2 |
DCP 5 |
38.3 |
DCP 25 |
85.7 |
Table: Specific respiration rates in the main test
Test assay nominal [mg/L] |
Specific respiration rates after 3 h [mg O2/(g × h)] |
Control (1) |
24.11 |
Control (2) |
25.04 |
Control (3) |
18.53 |
Mean |
22.56 |
Stdev |
3.52 |
CV (%) |
16 |
100 (1) |
22.57 |
100 (2) |
24.65 |
100 (3) |
26.07 |
Mean |
24.43 |
Stdev |
1.76 |
DCP 0.2 |
23.11 |
DCP 1 |
18.50 |
DCP 5 |
17.09 |
DCP 25 |
2.73 |
Abiotic control (1) |
-0.19 |
Abiotic control (2) |
0.35 |
Abiotic control (3) |
0.01 |
Table: Inhibition of total oxygen uptake in the main test
Test assay nominal [mg/L] |
Mean inhibition after 3 h [%] |
100 |
-8.3 |
DCP 0.2 |
-2.4 |
DCP 1 |
18.0 |
DCP 5 |
24.2 |
DCP 25 |
87.9 |
Description of key information
NOEC (3h) = 100 mg/L with activated sludge (nominal, OECD 209)
Key value for chemical safety assessment
Additional information
One study investigating the toxicity of Reaction mass of ammonium diaqua[bis(oxalate)]oxoniobate(1-) hydrate and ammonium hydrogen oxalate oxalic acid (1:1:1) dihydrate to microorganisms is available. The limit test at 100 mg/L test substance concentration was performed with activated sludge in a static test design according to OECD 209 (CBMM Europe BV, Key, 2014, Activated sludge). Mean specific respiration rates in the control and in the 100 mg/L test item concentration were 22.56 and 24.43 mg O2/(g x h), respectively. No significant inhibition occurred, resulting in a NOEC of 100 mg/L.
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