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EC number: 243-325-5 | CAS number: 19800-42-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- From March 19, 1976 to March 26, 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- No analytical verification of test concentrations
- Principles of method if other than guideline:
- Golden orfe (Leuciscus idus melanotus) were acclimated for at least 14 days before test begin. The animals were fed during the acclimation period but not during the study. Approximately 65 h before test begin, 10 fish/group were placed in the respective glass aquaria (40 x 25 x 30 cm) filled with 20 L of water and aerated at a rate of 120 ml/min. The test medium was deionised water containing 30 mg/L MgSO4, 48 mg/L Na2CO3, 3 mg/L KCl and 38 mg/L CaSO4.2H2O. The test substance, in the form of a yellow-brown viscous liquid, was dispersed in the test medium at nominal concentrations of 0, 10, 100 and 500 mg/L. Fish were observed daily for mortality and clinical signs during 96 h. Oxygen, pH and temperature were monitored daily. At the end of the study, all fish were sacrificed, dissected and macroscopically evaluated.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Leuciscus idus melanotus
- Details on test organisms:
- Body weight and length 1.0-3.6 g and 5.2-6.9 cm, respectively.
10 animals per dose - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 20.0 +/- 0.5°C
- pH:
- 7.20 - 7.95
- Dissolved oxygen:
- 7.30 - 8.40 mg/L
- Nominal and measured concentrations:
- Nominal: 0, 10, 100 and 500 mg/L, equivalent to 0, 3.3, 33 and 165 mg a.i./L based on a 33% test substance purity
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 165 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- 33% test substance purity
- Basis for effect:
- mortality (fish)
- Details on results:
- There was no mortality during the study and no behavioural abnormalities were noted.
At 100 and 500 mg/L (33 and 165 mg a.i./L), an orange-brown discoloration of the bile and yellowish liquid were observed in the gastrointestinal tract. There were no findings in the other dose groups. - Validity criteria fulfilled:
- not applicable
- Conclusions:
- Under the study conditions, the 96h LC50 of the test substance to golden orfe (Leuciscus idus f. melanotus) was determined to be > 500 mg/L, equivalent to > 165 mg a.i./L.
- Executive summary:
A study was conducted to determine the toxicity of the test substance (in the form of a water soluble yellow-brown viscous liquid of 33% purity) to Golden orfe (Leuciscus idus melanotus). After acclimation, 10 fish/group were exposed to the substance dispersed in test medium at nominal concentrations of 0, 10, 100 and 500 mg/L (equivalent to 0, 3.3, 33 and 165 mg a.i./L). The animals were observed daily for mortality and clinical signs during 96 h. Oxygen, pH and temperature were monitored daily. At the end of the study, all fish were sacrificed, dissected and macroscopically evaluated. No analytical validation of test concentrations was conducted. There was no mortality during the study and no behavioural abnormalities were noted. At 100 and 500 mg/L (33 and 165 mg a.i./L), an orange-brown discoloration of the bile and yellowish liquid were observed in the gastrointestinal tract. There were no findings in the other dose groups. Under the study conditions, the nominal 96 h LC50 was determined to be > 500 mg/L, equivalent to > 165 mg a.i./L (Markert, 1976). The study was repeated under the same conditions in 1977 and similar results were obtained (Report 521/77, Markert, 1977).
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From February 12, 1990 to February 16, 1990 and from March 12, 1990 to March 16, 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- No analytical verification of test concentrations
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- water
- Details on test solutions:
- The test substance was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Supplier/Breeder: West aquarium GmbH U. Co. KG
Body length and weight: 2.4-3.0 cm and 0.12-0.26 g, respectively. Loading: 0.34 g fish/L
Acclimatation: 1 week
Photoperiod: 16h light and 8h dark
Diet: "tetra min" std feed for aquarium fish, ad libitum - Test type:
- flow-through
- Water media type:
- freshwater
- Remarks:
- slight continuous aeration
- Total exposure duration:
- 96 h
- Hardness:
- ca. 2.5 mmol/L
- Test temperature:
- 21 +/- 1°C
- pH:
- ca. 8.0
- Dissolved oxygen:
- > 60% of maximum saturation
- Salinity:
- The resalting was carried out by addition of:
- 294.0 mg/L CaCl2.2H2O, 123.3 mg/L MgSO4.7H2O, 63.0 mg/L NaHCO3, and 5.5 mg/L KCl - Nominal and measured concentrations:
- Nominal: 0, 1000, 1800, 3200 and 5000 mg/L, equivalent to 0, 200, 360, 640 and 1000 mg a.i./L based on a purity of 20%
- Details on test conditions:
- Based on the results of 2 range finding studies
10 fishes per condition - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2 364 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 473 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- 20% test substance purity
- Duration:
- 96 h
- Dose descriptor:
- other: LC5
- Effect conc.:
- 1 128 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: LC5: 226 mg/L (a.i.)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 4 956 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: LC100: 991 mg/L (a.i.)
- Details on results:
- No symptoms were observed at any time and concentration tested.
Exposure to the test substance at concentrations of 1800, 3200 and 5000 mg/L induced mortality after 72 and 96h (all fish died at the concentration 5000 mg/L after 96 h of exposure). - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 96 h LC5, 50 and 100 of the test substance to Zebra fish (Brachydanio rerio) were determined to be 1128, 2364 and 4956 mg/L (nominal), respectively (corresponding to 225.6, 472.8 and 991.2 mg a.i./L).
- Executive summary:
A study was conducted to determine the toxicity of the test substance (in the form of a water soluble liquid dispersion of 20% purity) to Zebra fish (Brachydanio rerio) according to OECD Guideline 203. The fish were exposed for 96 h to nominal concentrations of 0, 1000, 1800, 3200 and 5000 mg/L (equivalent to 0, 200, 360, 640 and 1000 mg a.i./L). Mortality and symptoms were assessed daily. No analytical verification of test concentrations was conducted. There were no symptoms observed in any group. Exposure at 1800, 3200 and 5000 mg/L induced mortality after 72 and 96 h. Under the study conditions, the nominal 96 h LC5, 50 and 100 were determined to be 1128, 2364 and 4956 mg/L, respectively (corresponding to 226, 473 and 991 mg a.i./L) (Munk, 1990).
Referenceopen allclose all
Description of key information
Key value for chemical safety assessment:
LC50 for freshwater fish: 473.0 mg a.i./L
LC50 for marine fish: -
Key value for chemical safety assessment
Additional information
A study was conducted to determine the toxicity of the test substance (in the form of a water soluble liquid dispersion of 20% purity) to Zebra fish (Brachydanio rerio) according to OECD Guideline 203. The fish were exposed for 96 h to nominal concentrations of 0, 1000, 1800, 3200 and 5000 mg/L (equivalent to 0, 200, 360, 640 and 1000 mg a.i./L). Mortality and symptoms were assessed daily. No analytical verification of test concentrations was conducted. There were no symptoms observed in any group. Exposure at 1800, 3200 and 5000 mg/L induced mortality after 72 and 96 h. Under the study conditions, the nominal 96 h LC5, 50 and 100 were determined to be 1128, 2364 and 4956 mg/L, respectively (corresponding to 226, 473 and 991 mg a.i./L) (Munk, 1990).
A study was conducted to determine the toxicity of the test substance (in the form of a water soluble yellow-brown viscous liquid of 33% purity) to Golden orfe (Leuciscus idus melanotus). After acclimation, 10 fish/group were exposed to the substance dispersed in test medium at nominal concentrations of 0, 10, 100 and 500 mg/L (equivalent to 0, 3.3, 33 and 165 mg a.i./L). The animals were observed daily for mortality and clinical signs during 96 h. Oxygen, pH and temperature were monitored daily. At the end of the study, all fish were sacrificed, dissected and macroscopically evaluated. No analytical validation of test concentrations was conducted. There was no mortality during the study and no behavioural abnormalities were noted. At 100 and 500 mg/L (33 and 165 mg a.i./L), an orange-brown discoloration of the bile and yellowish liquid were observed in the gastrointestinal tract. There were no findings in the other dose groups. Under the study conditions, the nominal 96 h LC50 was determined to be > 500 mg/L, equivalent to > 165 mg a.i./L (Markert, 1976). The study was repeated under the same conditions in 1977 and similar results were obtained (Report 521/77, Markert, 1977; results not shown).
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