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Diss Factsheets
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EC number: 944-042-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From July 17, 2000 to March 16, 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- other: Royal Decree 363/1995 (BOE no. 133 of June 1995).
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Strauss (Cladocera, Crustacea)
- Source: University of Stirling UK
- Age at study initiation: < 24 hours
- Feeding during test: no
- Food type: Chlorella (freshwater microalgae) and seaweed extract.
ACCLIMATION
- Temperature: 18 - 22 ºC
- Type of food: Chlorella (freshwater microalgae) and seaweed extract.
- Feeding frequency: three time per week.
- Photoperiod: 16 hours light / 8 hours of darkness. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20 ± 2 ºC
- pH:
- 7.96 - 7.97
- Dissolved oxygen:
- 8.08 - 8.21 mg/l O2
- Nominal and measured concentrations:
- NOMINAL: 0.1 mg/l, 1 mg/l, 10 mg/l, 100 mg/l and 1000 mg/l
MEASURED: 0.1 mg/l, 1 mg/l, 10 mg/l, 100.1 mg/l and 1000.8 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: 40 ml tube
- Type (delete if not applicable): closed
- Material, size: 10 ml
- Aeration: no, closed with parafilm.
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- Photoperiod: continuous darkness.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD 202
— Solution 1: CaCl 2 x 2H2O 11,76 g/l H2O
— Solution 2: MgSO4 x 7H2O 4,93 g/l H2O
— Solution 3: NaHCO3 2,59 g/l H2O
— Solution 4: KCl 0,23 g/l H2O - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 1.03 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- EC50 (24 h) = 1,1 mg/l
- Conclusions:
- The substance was tested for short term toxicity to invertebrates following OECD 202. Under the experimental conditions the EC50 (48 h) equal to 1.03 mg/l, nominal
- Executive summary:
- The substance has been tested for short-term toxicity on freshwater invertebrates according to the guideline OECD 202 (1984) and Royal Decree 363/1995 (BOE no.133 of June 1995).
The freshwater crustacean Daphnia magna has been subjected to the dye at concentrations of: 0.1mg/L, 1 mg/L, 10 mg/L, 100mg/L and 1000 mg/L of active ingredient of that product. The total test duration was 48 hours.
The result obtained in this test indicates that the dye had a nominal value of EC50 (48 h) equal to 1.03 mg/L.
Reference
Inhibition of mobility
Conc. mg/l | % Inhibition mobility (48h) |
Control | 0 |
0.1 | 15 |
1 | 55 |
10 | 85 |
100 | 90 |
1000 | 100 |
Description of key information
EC50 (48 h) = 1.03 mg/l
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1.03 mg/L
Additional information
OECD 202 (1984)
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.