4-aminonaphthalene-1,8-dicarboximide

Biodegradation in water: screening tests

Description of key information

Estimation Programs Interface Suite (EPI suite, 2016) was run to predict the biodegradation potential of the test compound 4-Amino-1,8 -naphthalimid e (CAS no. 1742-95-6) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI LInear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that 4-Amino-1,8-naphthalimide is not expected to be readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

The predicted data for the test compound4-Amino-1,8-naphthalimide(CAS No. 1742-95-6) and the study for its read across substance were reviewed for the biodegradation end point which are summarized as below:

Estimation Programs Interface Suite (EPI suite, 2016) was run to predict the biodegradation potential of the test compound 4-Amino-1,8 -naphthalimid e (CAS no. 1742-95-6) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradatio n Timeframe, MITI LInear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that 4-Amino-1,8-naphthalimide is not expected to be readily biodegradable.

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance1,5-Naphthalenediamine (J-CHECK, 2016). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 0% by BOD and 3% by HPLC in 28 days. Thus, the substance1,5-Naphthalenediamine is not considered to be readily biodegradable in water.

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance1-Naphthylamine (J-CHECK, 2016). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 0% by BOD and 3% by HPLC in 28 days. Thus, the substance1-Naphthylamine is not be considered to be readily biodegradable in water.

Biodegradation study was conducted for evaluating the percentage biodegradability of test substance1-Naphthylamine (Environmental Fate Data Base, 2016). Concentration of inoculum i.e, activated sludge used was 100 mg/l and initial test substance conc. used in the study was 200 mg/l. The percentage degradation of test substance was determined to be 0% by COD removal in 5 days at a temperature of 20°C. Thus, the substance 1-Naphthylamine is not considered to be readily biodegradable in water.

Biodegradation study was conducted for evaluating the percentage biodegradability of test substance1-Naphthylamine (George W. Malaney, 1960). Aniline-acclimated sludge was used as a test inoculum. The original source of microorganisms was mixed liquor from the aeration tank of a municipal treatment plant. Biodegradation of test chemical was tested by the conventional Warburg technique, using 125 ml flasks. Concentration of inoculum i.e, sludge used was 2,500 mg/l and initial test substance conc. used in the study was 500 mg/l. Incubation of the test vessel was carried out at 20ᵒC for 5-8 days i.e, 120-192 hrs. The percentage degradation of test substance was determined to be 0% by BOD and 3% by HPLC in 28 days. Oxidation in the test flask was recorded as mg O2 uptake per liter of the mixture in the flask. The mg of O2 uptake by the test substance1-Naphthylaminewas determined to be 890 mg/l and the percentage degradation of the test substance was determined to be 23% by BOD in 8 days. Thus, the substance1-Naphthylamine is not expected to be readily biodegradable in water.

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance 1,8-Naphthalenediamine (J-CHECK, 2016). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 0% by BOD, 9% by TOC removal and 1% by HPLC in 28 days. Thus, the substance 1,8-Naphthalenediamine is not considered to be readily biodegradable in water.

On the basis of above results for target and read across substance, it can be concluded that the test substance compound 4-Amino-1,8-naphthalimide can be expected to be not readily biodegradable in nature.