N,N,N-trimethylanilinium chloride

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

of read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on toxicity study of microorganism for the test chemical :
1) luminiscence reduction test was performed for test material on Photobacterium phosphoreum.
2)To evaluate antibacterial activity of test chemical

GLP compliance:

not specified

Test organisms (species):

other: 1) P. phosphoreum 2) Bacillus subtilis

Test type:

static

Water media type:

freshwater

Total exposure duration:

30 min

Test temperature:

1) 15 deg C

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

0.22 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

other: Luminiscent

Remarks on result:

other: not specified

Dose descriptor:

other: IC50

Effect conc.:

500 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: not specified

Validity criteria fulfilled:

not specified

Conclusions:

The effect of test material on bioluminence of bacterial P.phosphorium and growth inhibition of Bacillus subtilis was observed to be 0.22 mg/l and 500 mg/l respectively.

Executive summary:

The effect of test material was evaluated on microorganisms ,Microtox bioassay was performed for the test material onP. phosphoreumbased on the light emission of these bacteria, as a measurement of their metabolic activity. 50% reductionof the light emitted by the bacteria (EC50) is calculated from a concentration response curve by regression analysis. The osmotic pressure of the samples was adjusted by NaCl addition (2%). Toxicity data were based on a 30-min exposure of bacteria to the surfactantsolution at 15 deg C.The effect concentration  EC50 of test material on  P. phosphoreum was evaluated to be 0.22 mg/l.

In another study , the test chemical was used to evaluate minimum inhibition concentration on the test organism Bacillus subtilis after inoculation of test substance , the effect concentration was observed to be 500 mg/l. The effect of test material on bioluminence of bacterial P.phosphorium and growth inhibition of Bacillus subtilis was observed to be 0.22 mg/l and 500 mg/l respectively.

Description of key information

Toxicity to microorganism:

The effect of test material was evaluated on microorganisms ,Microtox bioassay was performed for the test material onP. phosphoreumbased on the light emission of these bacteria, as a measurement of their metabolic activity. 50% reductionof the light emitted by the bacteria (EC50) is calculated from a concentration response curve by regression analysis. The osmotic pressure of the samples was adjusted by NaCl addition (2%). Toxicity data were based on a 30-min exposure of bacteria to the surfactantsolution at 15 deg C.The effect concentration  EC50 of test material on  P. phosphoreum was evaluated to be 0.22 mg/l.

In another study , the test chemical was used to evaluate minimum inhibition concentration on the test organism Bacillus subtilis after inoculation of test substance , the effect concentration was observed to be 500 mg/l. The effect of test material on bioluminence of bacterial P.phosphorium and growth inhibition of Bacillus subtilis was observed to be 0.22 mg/l and 500 mg/l respectively.

Additional information

Toxicity to microorganism:

The effect of test material was evaluated on microorganisms ,Microtox bioassay was performed for the test material onP. phosphoreumbased on the light emission of these bacteria, as a measurement of their metabolic activity. 50% reductionof the light emitted by the bacteria (EC50) is calculated from a concentration response curve by regression analysis. The osmotic pressure of the samples was adjusted by NaCl addition (2%). Toxicity data were based on a 30-min exposure of bacteria to the surfactantsolution at 15 deg C.The effect concentration  EC50 of test material on  P. phosphoreum was evaluated to be 0.22 mg/l.

In another study , the test chemical was used to evaluate minimum inhibition concentration on the test organism Bacillus subtilis after inoculation of test substance , the effect concentration was observed to be 500 mg/l. The effect of test material on bioluminence of bacterial P.phosphorium and growth inhibition of Bacillus subtilis was observed to be 0.22 mg/l and 500 mg/l respectively.