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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 December 2014
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Not GLP Compliant
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- The study procedures described in this report are a simplified version of the Organization for Economic Co-operation and Development (OECD), OECD guidelines for Testing of Chemicals, Section 2: Effects on biotic systems, Guideline no. 209, "Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation), adopted 22 July 2010.
- Deviations:
- no
- Principles of method if other than guideline:
- A concentration of 2.0 mg/l was tested instead of 1.0 mg/l. To the weighed test substance 400 ml Milli-RO should have been added and after stirring half of the suspension should be removed. However, 200 ml Milli-RO was added and no test suspension was removed after the 24 hour stirring period.
Evaluation: No biologically relevant effects were observed at any of the tested loading rates and therefore the results were not influenced. - GLP compliance:
- no
- Remarks:
- Study not done for EU Reach as an intermediate under SCC but to evaluate the test substance for its ability to adversely affect aerobic microbial treatment plants.
Test material
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Purity 99.7%
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The pH was determined in the remaining part of the reaction mixture. This procedure was repeated for all test/reference substance concentrations and controls.
The medium temperature was recorded continuously in a temperature control vessel(s). The temperature control vessel(s) was/were identically prepared compared to the control vessels. A temperature control vessel with a REES sensor was placed in each fume cupboard of the climate room.
Oxygen recording Determination of oxygen was performed with multiple oxygen probes connected to a BlueBox (GOSystemelektronik GmbH, Germany), a multichannel measuring and controlling system.
5.6. Electronic data capture
Observations/measurements in the study were recorded electronically using the following programmes:
• REES Centron Environmental Monitoring system version SQL 2.0 (REES Scientific, Trenton, NJ, USA): Temperature.
• BlueBox Software version 3.4.2.0. (GO-Systemelektronik GmbH, Germany): Oxygen.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The test substance was not sufficiently soluble to allow the preparation of a 10 g/l stock solution in water. Therefore, 1-Litre test bottles were filled with 200 ml of test substance mixtures in Milli-RO water (tap-water purified by reverse osmosis; Millipore Corp., Bedford, Mass., USA) with initial loading rates of 2.5 times the final loading rate. These mixtures were stirred in closed dark brown bottles for 25½ hours. Subsequently, 16 ml synthetic medium made up to 50 ml with Milli-RO water and 250 ml sludge were added resulting in the required loading rates (see also paragraph 5.3). Optimal contact between the test substance and test organisms was ensured applying continuous aeration and stirring.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
Study design
- Test type:
- other: Screening
- Water media type:
- other: Tap-water purified by reverse osmosis (Millipore Corp., Bedford, Mass., USA).
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- 3 hours, during which aeration and stirring took place.
- Post exposure observation period:
- After the 3-hour contact time, the oxygen consumption was recorded for a period of approximately 10 minutes
Test conditions
- Test temperature:
- 21.4 - 22.1 °C
- pH:
- The pH in all test vessels, before addition of sludge was between 7.5 and 7.6. After the 3 hour exposure period the pH was between 7.2 and 8.0.
- Dissolved oxygen:
- The aeration was adjusted in such a way that the dissolved oxygen concentration at the start was above 60-70% saturation (60% of air saturation is > 5 mg/l at 20°C) and to maintain the sludge flocs in suspension.
- Salinity:
- In the synthetic medium there was 0.7 g NaCl, 0.4 g CaCl2.2H2O, 0.2 g MgSO4.7H2O, and 2.8 g K2HPO4 dissolved in Milli-RO water
- Details on test conditions:
- Preparation of the sludge:
The sludge was coarsely sieved (1 mm) and allowed to settle. The supernatant was removed and ISO-medium was added. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.0 g/l of sludge, as used for the test). The pH was 6.0 and adjusted to 7.1 using a sodium bicarbonate solution (70 g/l, Sigma Aldrich, St. Louis, USA) on the day of testing. The batch of sludge was used one day after collection; therefore 50 ml of synthetic medium (=sewage feed) was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated
at test temperature until use.
Medium:
Adjusted ISO medium, formulated using RO-water (tapwater purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands) with the following composition:
CaCl2.2H2O 211.5 mg/l
MgSO4.7H2O 88.8 mg/l
NaHCO3 46.7 mg/l
KCl 4.2 mg/l - Reference substance (positive control):
- yes
- Remarks:
- The batch of activated sludge was checked for sensitivity by testing the reference substance 3,5-dichlorophenol.
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.04 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- Replicate Loading rate pH Respiration rate % Inhibition respiration
(mg/l) Start End (mg O2/l.h) (mg O2/g.h)¹ rate(mean value)
R1 2.0 7.6 7.9 31 21 30
R2 5.0 7.6 8.0 23 15 48
R3 12 7.6 8.0 13 9 71 - Reported statistics and error estimates:
- Table 4 Probit analysis 3,5-dichlorophenol
Parameter Value
Computation runs: 4.0000
Slope b: 1.3693
Intercept a: -0.96209
Variance of b: 4.3890
Goodness of Fit
Chi²: 0.0030
Degrees of freedom: 1.0000
p(Chi²): 0.9567
Log EC50: 0.7026
SE Log EC50: 0.4614
g-Criterion: 1.1166
Residual Variance (Chi²/df): 0.0030
r²: 0.9930
F: 144.5900
p(F) (df: 1;1): 0.0530
Any other information on results incl. tables
Replicate Loading rate pH Respiration rate % Inhibition respiration
(mg/l) Start End (mg O2/l.h) (mg O2/g.h)¹ rate(mean value)
T1 2.0 7.5 7.4 45 (=RT) 30 -2 (=IT)
T2 10 7.5 7.4 47 (=RT) 31 -6 (=IT)
T3 100 7.5 7.3 44 (=RT) 29 1 (=IT)
T4 1000 7.5 7.2 47 (=RT) 31 -6 (=IT)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- no
- Conclusions:
- Under the conditions of this present test PF-05029508 was not toxic to waste water bacteria (activated sludge) at a loading rate of 1000 mg/l.
The EL50 was above 1000 mg/l.
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