5-chloro-2-(2,4-dichlorophenoxy)aniline

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

5-chloro-2-(2,4-dichlorophenoxy)aniline

EC Number:

260-480-4

EC Name:

5-chloro-2-(2,4-dichlorophenoxy)aniline

Cas Number:

56966-52-0

Molecular formula:

C12H8Cl3NO

IUPAC Name:

5-chloro-2-(2,4-dichlorophenoxy)aniline

Details on test material:

- Name of test material (as cited in study report): FAT 80023/G
- Physical state: solid
- Analytical purity: 100%
- Batch No.: ICMD CH 2.23
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Product Ecology TF, Textile Dyes Devision
- Method of breeding: Cultures were held in glass vessels containing approx. 3 L of reconstituted water at 18-22°C. The water was renewed partially three times per week.
- Feeding during breeding:
- Food type: suspension of green algae (Scenedesmus subspicatus) supplemented by a TETRAMIN-extract
- Amount: quantities consumed within 24 hours
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

249 mg/L CaCO3

Test temperature:

20.8 - 21.1 °C

pH:

7.6 - 7.7

Dissolved oxygen:

96 - 98%

Nominal and measured concentrations:

Nominal test concentrations: control, 0.18, 0.4, 0.9, 1.9, 4.3 mg/L
Measured test concentrations: n.m., n.m., n.m., < 0.06, 0.17, 0.4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 ml beakers containing 80 ml test medium
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: bidistilled water
- Alkalinity: 0.8 mmol/L
- Ca/Mg ratio: 4:1
- Intervals of water quality measurement: Temperature, oH, oxygen content after 0 and 48 h

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light/ 12 hours darkness

EFFECT PARAMETERS MEASURED: Mobility after 24 and 48 hours

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

< 0.06 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

< 0.06 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

0.17 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Nominal effect concentrations (48 h):
- EC0 = 0.18 mg/L
- EC50 = 0.68 mg/L
- EC100 = 1.9 mg/L

- Immobility of control: none

Results with reference substance (positive control):

EC50 = 1.13 mg/L (control experiment 05 Oct 1995)