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EC number: 242-828-7 | CAS number: 19125-99-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Short term toxicity to fish:
The Lethal concentration (LC50)
value for 2-butyl-6-(butylamino)-1H-benzo[de]isoquinoline-1,3(2H)-dione
on Danio rerio (previous name: Brachydanio rerio) in a 96 hrs. study was
estimated to be 172.24 mg/L on the basis of effects on mortality in
static freshwater by OECD QSAR toolbox.
Short term toxicity to Aquatic
Invertebrates:
The median effective
concentration (EC50) for the test substance,
2-butyl-6-(butylamino)-1H-benz[de]isoquinoline-1,3(2H)-dione, in Daphnia
magna was determined to be 0.17 mg/L on the basis of mobility inhibition
effects in a 48 hour study.
Toxicity to Aquatic Algae and
Cyanobacteria:
The median effective
concentration (ErC50) for the test substance,
2-butyl-6-(butylamino)-1H-benz[de]isoquinoline-1,3(2H)-dione, in a
freshwater algae Desmodesmus subspicatus was determined to be 381.3 mg/L
on the basis of effects on growth rate in a 72 hour study.
Toxicity to microorganism:
The study does not need to be
conducted because the substance is highly insoluble in water, hence
indicating that aquatic toxicity is unlikely to occur.
Additional information
Short term toxicity to fish:
Predicted data for the substance
2-butyl-6-(butylamino)-1H-benzo[de]isoquinoline-1,3(2H)-dione and its
read across substance were reviewed for short term toxicity to fish
endpoint and are represented here as weight of evidence approach:
The toxic effects of the test compound 2-butyl-6-(butylamino)-1H-benzo[de]isoquinoline-1,3(2H)-dione were predicted using QSAR toolbox version 3.4. The prediction was based on the effects of the test compound on Danio rerio (previous name: Brachydanio rerio) in a static fresh water system during a 96 hour exposure. The Lethal concentration (LC50) value was estimated to be 172.24 mg/L on the basis of effects on mortality.
96 hour short term toxicity to fish was carried for the substance 2-methyl-1H-isoindole-1,3(2H)-dione (CAS 550-77-4)according to OECD guideline 203 (HPVIS database, 2017). The test species used was fresh water Oncorhynchus mykiss in static condition. Nominal concentrations used were 0, 40, 68,116, 200, 293.3 mg/L while measured concentrations were 0, 34.6, 58.7, 100.3, 174.6, 293.3 mg/L. LC50 were calculated at 24, 48, 72, and 96 h. At test termination (96-hour exposure), all fish appeared normal in the control. As per the observations made, the 50% Lethal concentration value of the substance 2-methyl-1H-isoindole-1,3(2H)-dione to Oncorhynchus mykiss is determined to be 110 mg/L.
Considering above data and by applying
weight of evidence approach it can be concluded that the substance
2-butyl-6-(butylamino)-1H-benzo[de]isoquinoline-1,3(2H)-dione is not
toxic to fish and is considered to not classified as per CLP regulation.
Short term toxicity to Aquatic
Invertebrates:
Determination of the inhibition
of the mobility of daphnids was carried out with the substance,
2-butyl-6-(butylamino)-1H-benz[de]isoquinoline-1,3(2H)-dione according
to OECD Guideline 202. The test substance was tested at the
concentrations 0, 0, 0.025, 0.05, 0.10, 0.25 and 0.50 mg/L. Effects on
immobilisation were observed for 48 hours. The median effective
concentration (EC50) for the test substance,
2-butyl-6-(butylamino)-1H-benz[de]isoquinoline-1,3(2H)-dione, in Daphnia
magna was determined to be 0.17 mg/L. Thus considering the above value,
it can be concluded that 2-butyl-6-(butylamino)-
1H-benz[de]isoquinoline-1,3(2H)-dione exhibits toxicity to aquatic
invertebrate (Daphnia magna) in the acute 1 classification category.
Also since long term toxicity data is not available and the substance is
not readily biodegradable, the final classification for aquatic
invertebrate is considered to be Aquatic Acute 1 as well as Chronic
category 1 as per CLP criteria.
Toxicity to Aquatic Algae and
Cyanobacteria:
Freshwater algal growth
inhibition test was carried out on Desmodesmus subspicatus with the
substance 2-butyl-6-(butylamino)-1H-benz[de]isoquinoline -1,3(2H)-dione
according to OECD Guideline 201. The test substance was dissolved in
DMSO and tested at the concentrations 0, 0, 5, 10, 20, 40 and 50 mg/L.
Effects on the growth rate of the organism were studied. The median
effective concentration (ErC50) for the test substance,
2-butyl-6-(butylamino)-1H-benz[de]isoquinoline-1,3(2H)-dione, in
Desmodesmus subspicatus was determined to be 381.3 mg/L. Based on this
ErC50 value and after comparing with CLP criteria for aquatic
classification of the substance it is concluded that the substance
2-butyl-6-(butylamino)-1H-benz[de]isoquinoline-1,3(2H)-dione does not
exhibit toxicity to aquatic algae (Desmodesmus subspicatus).
Toxicity to microorganism:
The study does not need to be
conducted because the substance is highly insoluble in water, hence
indicating that aquatic toxicity is unlikely to occur.
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