N-[2-[(2-cyano-4,6-dinitrophenyl)azo]-5-(diethylamino)phenyl]acetamide

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experimental Starting Date: 23/02/2015; Experimental Completion Date: 27/02/2015; Study Completion Date: 05/03/2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

Principles of method if other than guideline:

None

GLP compliance:

no

Type of method:

flask method

Specific details on test material used for the study:

Identification: FAT 36156/D TE
Lot. 20140804
Appearance: Blue powder

Water solubility:

< 0.2 mg/L

Temp.:

20 °C

Remarks on result:

other: Without correction of the purity.

Details on results:

None

Individual Results

Preliminary Visual Estimation of the Water Solubility

Table 1: Appearance of the test mixtures in the preliminary test

Amount of the test item in mg	Total volume in ml	Appearance of mixture
	10	undissolved
10	50	undissolved
	100	undissolved

The preliminary visual experiments showed that the water solubility of the test item is below 100mg/l

 Flask-Method

 

Table 2: Water solubility of the test item – experiments A to D

 

Experiment	A	B	C	D(blank)
Amount of test item(mg)	500	500	500	---
Stirring time at 30°C (h)	24	48	72	72
Measured concentration (µg/ml)	0.16	0.21	0.20	---
Aliquot	undiluted	undiluted	undiluted	undiluted
Water solubility (mg/l)	0.16	0.21	0.20	---

 

Average experiments A to C: 0.20 mg/l

 

Table 3 : Standard addition for recheck :

 

Standard	Measured concentration (µg/ml)	% Recovery
Sample 3 + 0.25 µg test item	0.46	102.2

 

The recovery of 102.2 % indicates a sufficient accuracy of the analytical method.

 

Conclusions:

The water solubility of FAT 36156/D TE at 20 °C was determined to be 0.20 mg/l.

Executive summary:

The purpose of the study was determination of the water solubility of the test item according to the European Commission Regulation (EC) No.440/2008 and A6. Water solubility, flask – method and OECD test guidelines OECD 105 water solubility were used to perform the experiment. Shake flask method was used for this purpose. Based on the findings of the study, the water solubility of FAT 36156/D TE at 20 °C was determined to be 0.20 mg/l.

Description of key information

The purpose of the study was determination of the water solubility of the test item according to the European Commission Regulation (EC) No.440/2008 and A6. Water solubility, flask – method and OECD test guidelines OECD 105 water solubility were used to perform the experiment. Shake flask method was used for this purpose. Based on the findings of the study, the water solubility of FAT 36156/D TE at 20 °C was determined to be 0.20 mg/l.

Key value for chemical safety assessment

Water solubility:

0.2 mg/L

at the temperature of:

20 °C