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EC number: 237-457-2 | CAS number: 13811-50-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993-06-02
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant and guideline similar study. Only 4 strains were tested.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- , 4 instead 5 strains tested
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,3-divinylimidazolidin-2-one
- EC Number:
- 237-457-2
- EC Name:
- 1,3-divinylimidazolidin-2-one
- Cas Number:
- 13811-50-2
- Molecular formula:
- C7H10N2O
- IUPAC Name:
- 1,3-diethenylimidazolidin-2-one
- Test material form:
- solid: flakes
- Details on test material:
- - Name of the test substance: N,N'-Divinylimidazolidon
- Batch no.: 26300/9
- Analytical purity: 96.3 %
- Date of manufacturing: 1993-01-20
- Appearance: Colorless to yellow flakes
- Storage: Refrigerator
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: TA 1535 and TA 100 used to detect base pair substitutions; TA 1537 and TA 98 are strains for the detection of frameshift mutagens (+1 mutant his C 3076 in the case of TA 1537 and the +2 type his D 3052 in the case of TA 98)
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor induced rat (male) liver S-9 mix
- Test concentrations with justification for top dose:
- 20, 100, 500, 2500, 5000 µg/plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: with metabolic activation: 2-aminoanthracene (all strains); without metabolic activation: N-methyl-N'-nitro-N-nitrosoguanidine (MNNG for TA 100 and TA 1535), 4-nitro-o-phenylendiamine (NPD for TA 98) and 9-aminoacridine chloride monohydrate (ACC, TA 1537)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: In agar (plate incorporation) and preincubation
Standard plate test:
2 mL portions of soft agar which consists of 100 mL agar and 10 mL amino acid solution are kept in a water bath at 45°C, the remaining components were added in the following order:
0.1 mL test solution or vehicle
0.1 mL bacteria] suspension
0.5 mL S-9 mix (in tests with metabolic activation)
or
0.5 mL phosphate buffer (in tests without metabolic activation )
After mixing, the samples were poured onto Vogel-Bonner agar plates (minimal glucose agar plates) within approx. 30 seconds.
Preincubation test:
0.1 mL test solution or vehicle, 0 .1 mL bacterial suspension and 0.5 mL S-9 mix are incubated at 37°C for the duration of 20 minutes. Subsequently, 2 mL of soft agar was added and, after mixing, the samples were poured onto the Vogel-Bonner agar plates within approx. 30 seconds.
After incubation at 37°C for 48 hours in the dark, the bacterial colonies (his revertants) were counted.
DURATION
- Exposure duration: 20 min
- Expression time: 48 hours
NUMBER OF REPLICATIONS: 3 test plates per dose or per control
DETERMINATION OF CYTOTOXICITY
- Method: Relative total growth
OTHER EXAMINATIONS:
Strains are checked for the following characteristics at regular intervals : deep rough character (rfa) ; UV sensitivity (A uvrB) ; ampicillin resistance (R factor plasmid).
Histidine auxotrophy is automatically checked in each experiment via the spontaneous rate. - Evaluation criteria:
- In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: A weakly bacteriotoxic effect was observed only with the strains TA 1535 and TA 100 (details see below).
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- An increase in the number of his+ revertants was not observed both in the standard plate test and in the preincubation test either without S-9 mix or after the addition of a metabolizing system.
TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: No test substance precipitation was found.
ADDITIONAL INFORMATION ON CYTOTOXICITY:
A slight decrease in the number of his(+) revertants was observed in the standard plate test and preincubation assay using TA 100 depending on the test conditions at doses >= 500 µg/plate.
A weakly bacteriotoxic effect was also found in the preincubation test without S-9 mix at 5000 µg/plate with TA 1535. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Number of revertants per strain (mean from three plates), Standard Plate Test
|
Strain TA 1535 |
Strain TA 1537 |
Strain TA 98 |
Strain TA 100 |
||||
Conc. [µg/plate] |
-S9 mix |
+S9 mix |
-S9 mix |
+S9 mix |
-S9 mix |
+S9 mix |
-S9 mix |
+S9 mix |
Positive control* |
90 |
785 |
317 |
88 |
725 |
561 |
723 |
478 |
Negative control |
20 |
19 |
10 |
12 |
34 |
44 |
114 |
129 |
20 |
23 |
19 |
8 |
11 |
29 |
39 |
113 |
110 |
100 |
25 |
20 |
11 |
12 |
38 |
27 |
110 |
108 |
500 |
20 |
19 |
9 |
10 |
32 |
33 |
120 |
91 |
2500 |
24 |
15 |
9 |
18 |
34 |
59 |
86 |
80 |
5000 |
23 |
18 |
8 |
14 |
29 |
42 |
74 |
75 |
*without S9 mix: MNNG (5 µg/plate): TA1535, TA 100; AAC (100 µg/plate): TA 1537; NPD (10 µg/plate): TA98
*with S9 mix: 2-AA (2.5 µg/plate): TA1535, TA 100; TA 1537, TA 98
Table 2: Number of revertants per strain (mean from three plates), Preincubation Test
|
Strain TA 1535 |
Strain TA 100 |
Strain TA 1537 |
TA 98 |
||||
Conc. [µg/plate] |
-S9 mix |
+S9 mix |
-S9 mix |
+S9 mix |
-S9 mix |
+S9 mix |
-S9 mix |
+S9 mix |
Positive control* |
827 |
93 |
1088 |
487 |
428 |
79 |
928 |
342 |
Negative control |
19 |
13 |
107 |
108 |
9 |
10 |
28 |
43 |
20 |
10 |
14 |
96 |
113 |
8 |
10 |
31 |
36 |
100 |
11 |
15 |
99 |
123 |
6 |
8 |
33 |
38 |
500 |
13 |
16 |
64 |
100 |
7 |
9 |
28 |
36 |
2500 |
14 |
12 |
69 |
100 |
12 |
10 |
33 |
35 |
5000 |
9 |
10 |
17 |
76 |
9 |
12 |
32 |
42 |
*without S9 mix: MNNG (5 µg/plate): TA1535, TA 100; AAC (100 µg/plate): TA 1537; NPD (10 µg/plate): TA98
*with S9 mix: 2-AA (2.5 µg/plate): TA1535, TA 100; TA 1537, TA 98
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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