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EC number: 810-021-4 | CAS number: 111304-31-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- This study followed the procedures indicated by the following internationally accepted gudelines and recommendations: - Ninth Addendum to OECD Guidelines for Testing of Chemicals, Section 4, No. 471, "Bacterial Reverse Mutation Test", adopted 21st July, 1997 - Council Regulation (EC) No 440/2008 B 13/14 Mutagenicity: Reverse Mutation Test Using Bacteria dated May 30, 2008 - EPA Health Effects Test Guidelines, OPPTS 870.5100 "Bacterial Reverse Mutation Test" EPA 712-C-98-247, August 1998 - ICH Guideance S2 (R1): Guideance on Genotoxicity testing and Data Interpretation for Pharmaceuticals Intended for Human Use, June 2012
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: CH Guideance S2 (R1): Guideance on Genotoxicity testing and Data Interpretation for Pharmaceuticals Intended for Human Use, June 2012
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- methyl (2Z)-2-(2-nitrobenzylidene)-3-oxobutanoate
- EC Number:
- 810-021-4
- Cas Number:
- 111304-31-5
- Molecular formula:
- C12H11NO5
- IUPAC Name:
- methyl (2Z)-2-(2-nitrobenzylidene)-3-oxobutanoate
- Test material form:
- solid: crystalline
- Details on test material:
- ame: Niliden
Cas no.: 111304-31-5
Batch no.: ND-78
Date of analysis: October 2014
Re-test date: October 2015
Storage: In well-closed vial, up to 25°C
Description: a white to pale yellow crystalline substance
Purity by HPLC: 99,6%
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- The test bacteria were also exposed to the test item in the presence of an appropriate metabolic activation system, which is a cofactor-supplemented post-mitochondrial fraction (S9).
- Test concentrations with justification for top dose:
- Concentration tested (µg/plate): 500,158, 50, 15.8, 5, 1.58
- Vehicle / solvent:
- Dimethyl sulfoxide (DMSO)
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Control groups: Dimethyl sulfoxide (DMSO), Ultrapure water
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- with and without metabolic activation
- Positive control substance:
- other: 4-nitro-1,2-phenylendiamine ; 2-aminoanthracene
- Details on test system and experimental conditions:
- The tester strains arrived to the test facility in a form of the disc cultures. The origin of the tester strains: S. typhimurium TA 98 and TA 100.
In addition to histidine mutation ,each strain has additional mutations which enhance its sensitivity to mutagens.
The strains are stored in the Laboratory of Toxi Coop Zrt. in the form of lyophilized discs and at (-) 80 ±10 °C in frozen permanent copies. Frozen permanent cultures of the tester strains are prepared from fresh, overnight cultures to which DMSO is added as cryoprotective agent. - Evaluation criteria:
- The test item is considered mutagenic if:
- a dose-related increase in the number of revertants occur and/or;
- a reproducible biologically relevant positive response for at least one of the dose groups occurs in at least one strain with or without metabolic activation.
Criteria for a negative response:
A test item is considered non-mutagenic if it produces neither a a dose-related increase in the number of revertants nor a reproducible biologically relevant positive response at any of the dose groups, with or without metabolic activation.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- No biologically relevant increases were observed in revertant colony numbers of nay of the examined strains following treatment with Niliden at any concentration level, either in the presence or absence of metabolic activation (S9 Mix) in the performed experiments.
The reference mutagen treatments ( positive controls) showed the expected, biological relevant increases in induced revertant colonies in all experimental phases, in both tester strains. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test item did not induce gene mutations by base pair changes or frameshifts in the genome of the strains used.
In conclusion, the test item Niliden has no mutagenic activity on the applied bacterium tester strains under the test conditions used in this study.
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