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EC number: 205-860-2 | CAS number: 156-60-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was selected as the key study because the information provided for the hazard endpoint is sufficient for the purpose of classification and labelling and/or risk assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- no
- Remarks:
- The study was conducted according to the guideline in effect at the time of study conduct.
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1300 (Acute inhalation toxicity)
- Deviations:
- no
- Remarks:
- The study was conducted according to the guideline in effect at the time of study conduct.
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.2 (Acute Toxicity (Inhalation))
- Deviations:
- no
- Remarks:
- The study was conducted according to the guideline in effect at the time of study conduct.
- Qualifier:
- according to guideline
- Guideline:
- other: MAFF Japan 59 NohSan No. 4200 Testing Guidelines for Toxicity Studies (1985)
- Deviations:
- no
- Remarks:
- The study was conducted according to the guideline in effect at the time of study conduct.
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- trans-dichloroethylene
- EC Number:
- 205-860-2
- EC Name:
- trans-dichloroethylene
- Cas Number:
- 156-60-5
- Molecular formula:
- C2H2Cl2
- IUPAC Name:
- (1E)-1,2-dichloroethene
- Details on test material:
- - Purity: 99.89%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Crl:CD®(SD)IGS BR
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: approximately 8 or 9 weeks old
- Weight at study initiation: Males - 233 to 348 grams; Females - 184 to 235 grams
- Housing: Rats were housed either singly or in pairs (sexes separate) in suspended, stainless steel, wire-mesh cages.
- Diet: PMI Nutrition International, Inc. Certified Rodent LabDiet® 5002, ad libitum
- Water: tap water from United Water Delaware, ad libitum
- Acclimation period: 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): targeted to be within a temperature range of 23 ± 1°C
- Humidity (%): 50 ± 10%
- Photoperiod: 12-hour light/12-hour dark cycle
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: Houseline nitrogen and air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: stainless steel and glass (NYU style)
- Exposure chamber volume: 150 L
- Method of holding animals in test chamber: rats were placed within wire-mesh cages and exposed whole-body inside the exposure chamber
- Source and rate of air: 37 L/min
- System of generating vapour: Chamber atmospheres were generated by flash evaporation of liquid test substance in nitrogen (N2 flow = 3 L/min). For each exposure, the subject test substance was metered into a heated (90-171°C), glass mixing flask with a Cole Palmer Masterflex® Console Drive pump. Houseline nitrogen was introduced to the heated mixing flask to carry the vapour into the exposure chamber. Houseline air was introduced before the exposure chamber to dilute the atmosphere generated at the flask.
- Temperature and humidity in air chamber: 22 to 26°C and 32 to 55%, respectively
TEST ATMOSPHERE
- Brief description of analytical method used: Chamber atmosphere samples were directly injected into a Hewlett Packard model 6890 Plus Series Gas Chromatograph equipped with a flame ionization detector for analysis at approximately 15-minute intervals during each exposure. Vapour samples were drawn by vacuum pump from representative areas of the chamber where rats were exposed. All samples were chromatographed isothermally at 100°C on a Restek RTX-200 column.
- Samples taken from breathing zone: yes
VEHICLE
- Composition of vehicle (if applicable): Houseline nitrogen and air - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 0, 12300, 22500, 28100 and 34100 ppm
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: Rats were observed for mortality and response to alerting stimuli during the exposure and observed for mortality and clinical signs of toxicity immediately after they were removed from the chambers following exposure. During a 14-day postexposure period, all surviving rats were observed each day for mortality, and were weighed and observed daily for clinical signs of toxicity.
- Necropsy of survivors performed: yes
- All rats were given a complete gross pathological examination of their internal organs including observation of the nasal passages. Representative samples of the liver, kidney, lung, and heart were saved at necropsy. All tissues were processed, embedded in paraffin, cut at a nominal thickness of 5 micrometers, stained with hematoxylin and eosin (H&E) and examined microscopically. - Statistics:
- Probit Analysis
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 24 100 ppm
- Based on:
- test mat.
- 95% CL:
- 19 200 - 26 900
- Exp. duration:
- 4 h
- Remarks on result:
- other: Diminished or a lack of response to an alerting stimulus observed during exposure at all doses
- Sex:
- male/female
- Dose descriptor:
- other: NOEL
- Effect level:
- 34 100 ppm
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Remarks on result:
- other: for gross and microscopic pathology; highest concentration tested
- Mortality:
- See Table in Results below. All deaths occurred during exposures. In calculating the LC50, the male and female rat lethality data was combined because there was no apparent sex difference in the lethality response to the tested compound.
- Clinical signs:
- other: During exposure, rats were prostrate, many had their eyes open, and exhibited a diminished or a lack of response to an alerting stimulus. After the 12300 ppm exposure, rats appeared to recover and resume a normal appearance within about thirty minutes af
- Body weight:
- Rats that survived the 22500 ppm exposure showed slight weight loss for one day followed by a normal weight-gain rate. Rats exposed to 28100 ppm showed slight to severe weight loss for one day.
- Gross pathology:
- There were no test substance-related gross observations.
Any other information on results incl. tables
VAPOUR CONCENTRATION |
MORTALITY (# deaths/# exposed) |
|
ppm |
MALES |
FEMALES |
Control |
0/5 |
0/5 |
12300 |
0/5 |
0/5 |
22500 |
1/5 |
3/5 |
28100 |
3/5 |
4/5 |
34100 |
5/5 |
5/5 |
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- This study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability).
LC50 = 24100 ppm - Executive summary:
Groups of 5 male and 5 female Crl:CD®(SD)IGS BR rats were exposed whole-body to the test item for a single 4-hour exposure period. A control group was treated similarly except for exposure to the test substance. The test atmosphere was generated by flash evaporating the liquid test substance in nitrogen. The concentration was determined by gas chromatographic analysis. During a 14-day recovery period, rats were weighed and observed for clinical signs of toxicity. All rats underwent gross pathologic examination immediately after death or at the end of the recovery period and the liver, kidney, heart, and lung were evaluated histologically. In calculating the LC50, the male and female rat lethality data was combined because there was no apparent sex difference in the lethality response to the test compound. The LC50 for the test item was 24100 ppm. All deaths occurred during exposures. During each exposure, the rats were prostrate, many had their eyes open, and showed a diminished or lack of response to an alerting stimulus.
After the 12300 ppm exposure, rats appeared to recover and resume a normal appearance within about thirty minutes after the end of the exposure. There were no effects on rat body weights at this concentration. Rats that survived the 22500 ppm exposure showed lethargy and irregular respiration immediately after exposure and showed slight weight loss for one day followed by a normal weight-gain rate. Rats exposed to 28100 ppm of showed weakness immediately after exposure and slight to severe weight loss for one day. There were no test substance-related gross or microscopic observations for the test substance.
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