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EC number: 942-532-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From February 12,2014 to March31,2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Test conducted according to internationally accepted testing guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Method for Testing the Biodegradability of Chemical Substances by Microorganisms
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- NACET00502
- IUPAC Name:
- NACET00502
- Reference substance name:
- 3-Methyl-5-(2,2,3-trimethylcyclopentyl)pentan-2-one
- Cas Number:
- 119464-63-0
- Molecular formula:
- C14H26O
- IUPAC Name:
- 3-Methyl-5-(2,2,3-trimethylcyclopentyl)pentan-2-one
- Test material form:
- other: light yellow oil
Constituent 1
Constituent 2
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): On-site sludge sampling was carried out at 10 locations in Japan (samples were from surface water and surface soil of rivers, lakes, and inland sea; return sludge from sewage plants).
- Laboratory culture: Activated sludge, which was prepared and controlled in this laboratory according to the test method described in OECD, was us ed in this study (sampling period: November, 2013, initiation date of use: December 20, 2013).
- Method of cultivation: The activated sludge, which was cultivated for 22 hours after the synthetic sewage was added.
- Preparation of inoculum for exposure: The synthetic sewage was prepared according to the following method; glucose, peptone, and potassium
dihydrogenphosphate were dissolved in purified water, and the pH of the solution was adjusted to 7.0 ± 1
- Pretreatment: Decision of additive amount of activated sludge
Additive amount of activated sludge into the test vessel was 2.51 mL on the basis of the concentration of suspended solid in the activated sludge
which was determined by the following methods:
Method In accordance with Japanese Industrial Standards (JIS) K 0102-2008 Section 14.1
Result 3590 mg/l
- Concentration of sludge: 30 mg/l - Duration of test (contact time):
- ca. 28 d
Initial test substance concentration
- Initial conc.:
- ca. 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The basal culture medium (4 L) was prepared at the same proportion as the following method; purified water (The Japanese Pharmacopoeia, Takasugi Pharmaceutical) was added to each 3 ml aliquot of solutions A, B, C and D, which are described in JIS K 0102-2008 Section 21, in order to prepare 1 l of solution.
- Test temperature: 25±1°C
- pH: The pH of this solution was then adjusted to 7.0.
- pH adjusted: yes
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: 3
CONTROL AND BLANK SYSTEM
- Inoculum blank: In one test vessel, nothing was added to the basal culture medium [the volume subtracting the volume (2.36 ml) of activated sludge from 300 ml].
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradationopen allclose all
- Parameter:
- other: BOD
- Value:
- ca. 6
- Sampling time:
- 28 d
- Parameter:
- % degradation (test mat. analysis)
- Remarks:
- GC
- Value:
- ca. 37
- Sampling time:
- 28 d
Any other information on results incl. tables
In the vessel Nos. 3 and 4, the percentage biodegradation by BOD was -3 % and -4 %, suggesting that the test item was not biodegraded. The percentage residue of the test item was good as 98 % and 97 %. From the result of the qualitative analysis for converted products by LC-MS, the converted product was not detected. Therefore, all the test item remained. Detected DOC (percentage detection: 4%) was thought to be derived from the test item dissolved in the test solution.
In vessel No. 2, the percentage biodegradation by BOD was 26 %, suggesting that some of the test item were biodegraded. The percentage residue of the test item was 0% and the peak derived from converted product was detected on the GC chromatogram. From the results of the qualitative analysis for converted products, the converted product A (2,2,3-trimethylcyclopentyl acetate) was detected. Detected DOC (percentage detection: 70 %) was thought to be derived from the converted product A dissolved in the test solution because the converted product A was detected in the sample for LC-MS -2 (for water-soluble converted products). The DOC value calculated from the theoretical amount of the converted product A was compared with experimental DOC value. As a result, the calculated DOC of 17.2 mgC was agreed with experimental value (1 6.8 mgC). Therefore, all of the test item was thought to be converted to the converted product A in the vessel No. 2, and then the produced converted product A was dissolved
in the test solution.
The percentage biodegradation by BOD was thought to be derived from the biodegradation of a part of the alkyl chain in the structural formula of the test item.
Sludge +test item | |||||
Vessel No. 2 |
Vessel No. 3 |
Vessel No. 4 |
average | ||
Percentage biodegradation by BOD |
% | 26 | -3 | 4 | 6 |
Percentage biodegradation of test item (by GC) |
% | 100 | 4 | 5 | 37 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Under the test conditions of this study, the test item was not biodegraded and remained in two of three test vessels. In another test vessel, the test item was completely converted and 2,2,3-trimethylcyclopentyl acetate was produced. 2,2,3-trimethylcyclopentyl acetate was not furher biodegraded.
- Executive summary:
The substance has been tested for ready biodegradation according to the Regulation OECD 301C.
Conditions of incubation:
Concentration of test item: 100 mg/L
Concentration of activated sludge: 30 mg/L (as concentration of suspended solid)
Volume of test solution: 300 mL
Incubation temperature: 25±1°C
Incubation duration: 28 days (under dark conditions)
Measurement and analysis for calculation of percentage biodegradation:
a) Measurement of biochemical oxygen demand (BOD) with a closed system oxygen consumption measuring apparatus
b) Determination of test item by gas chromatography (GC)
Under the test conditions of this study the test item was not biodegraded and remained in two of three test vessels. In another vessel the test item was completely converted and 2,2,3-trimethylcyclopentyl acetate was produced. 2,2,3-trimethylcyclopentyl acetate was not biodegraded.
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