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EC number: 220-688-8 | CAS number: 2867-47-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Original report is only available in Japanese. Translated report present.
Data source
Referenceopen allclose all
- Reference Type:
- other: summary report
- Title:
- Unnamed
- Year:
- 1 998
- Reference Type:
- publication
- Title:
- MHW, Japan (1998) Ministry of Health and Welfare, Toxicity Testing Reports of Environmental ChgmicaIs 6, 539-568
- Author:
- Ministry of Health and Welfare, Japan
- Year:
- 2 003
- Bibliographic source:
- cited in: OECD SIDS, 2-Dimethylaminoethylmethacrylate, CAS No: 2867-47-2, 07/2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-dimethylaminoethyl methacrylate
- EC Number:
- 220-688-8
- EC Name:
- 2-dimethylaminoethyl methacrylate
- Cas Number:
- 2867-47-2
- Molecular formula:
- C8H15NO2
- IUPAC Name:
- 2-(dimethylamino)ethyl methacrylate
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- TEST MATERIAL:
- Name of test material: 2-dimethylaminoethyl methacrylate
- Purity: 99.9%
- Impurities: Hydroquinone monomethyl ether (as polymerization inhibitor) 2000 ppm, dimethyl amino ethanol less than 0.1%, methylmethacrylate less than 0.02%
- Supplier: Sanyo Kasei Co.
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9: Rat liver, induced with phenobarbital and 5,6-benzoflavone
- Test concentrations with justification for top dose:
- Without S9 mix: 156, 313, 625, 1250, 2500, 5000 µg/plate
With S9 mix: 156, 313, 625, 1250, 2500, 5000 µg/plate
Confirmative test without S9 mix: 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, 5000 µg/plate - Vehicle / solvent:
- Distilled water
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Remarks:
- Without S9 mix: 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide (TA100, TA98, WP2 uvrA), Sodium azide (TA1535) and 9-Aminoacridine (TA1537). With S9 mix: 2-Aminoanthracene (all strains).
- Details on test system and experimental conditions:
- By the preliminary test to decide the highest concentration, toxicity was observed at 5000 ug/plate in the direct method without S9 mix for TA 98 and TA 1537. Then the highest concentration was set at 5000 µg/plate for all tests.
- Procedure: Pre-incubation method
- No. of replicates: 2
- No. of plates/test: 3
Two trial tests were done for all cells and a confirmation test was conducted for TA 98 and TA 1537 which showed positive results in the trial tests. - Evaluation criteria:
- 1) The revertant colony increase should be more than two times of the control.
2) The revertant colony increase should increase proportionally to the concentration of the test substance (concentration dependency).
3) The same revertant colony increase should be observed repeatedly by more than two tests.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- at 2500 and 3000 µg/plate
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- > 3500 µg/plate
- Additional information on results:
- In the two tests, the test substance caused a revertant colony increase of 2-times as much as that of the control to S. typhimurium TA 1537 at 2500 µg/plate without S9. However, a concentration dependency was not clear. Additionally, a revertant colony increasing tendency was observed for S. typhimurium TA98 at 2500 µg/plate and 5000 µg/plate without S9.
Consequently, to confirm the concentration dependent increase of revertant colonies at 2500 - 5000 µg/plate, the confirmation test was conducted for S. typhimurium strains TA 1537 and TA 98 by the direct method without S9. In this test, toxic effects were observed at 3500 µg/plate and more to TA 98 and TA 1537 without S9 mix. For TA 1537 a revertant colony increase was observed by more than 2-times of the control at 2500 and 3000 µg/plate. Furthermore, a concentration dependency was observed. For TA 98, although a revertant colony increase was observed at 2500 and 3000 µg/plate, it was less than 2-times as much as that of the control.
According to this study, the test substance is considered to be positive in this bacterial reverse mutation test. The number of the induced revertant colonies per mg was calculated to be 3.6/mg.
Any other information on results incl. tables
Number of revertant colonies (mean ± SD):
Dose/plate (µg) |
Without S9 mix |
With S9 mix |
||
Mean |
SD |
Mean |
SD |
|
1st Experiment |
||||
Salmonella typhimurium TA 98 |
||||
0 (Water) |
20 |
4 |
35 |
9 |
625 |
|
|
36 |
3 |
1250 |
20 |
4 |
42 |
7 |
2500 |
35 |
8 |
37 |
5 |
5000 |
34* |
14 |
48 |
9 |
AF-2 0.1 µg |
389 |
11 |
|
|
2-AA |
|
|
275 |
23 |
|
||||
Salmonella typhimurium TA 1537 |
||||
0 (Water) |
7 |
1 |
12 |
4 |
625 |
|
|
14 |
1 |
1250 |
7 |
1 |
15 |
2 |
2500 |
15 |
4 |
18 |
3 |
5000 |
4* |
2 |
17 |
3 |
9-AA 80 µg |
946 |
132 |
|
|
2-AA |
|
|
89 |
12 |
|
||||
2nd Experiment |
||||
Salmonella typhimurium TA 98 |
||||
0 (Water) |
26 |
6 |
37 |
7 |
625 |
|
|
35 |
5 |
1250 |
23 |
7 |
34 |
10 |
2500 |
39 |
2 |
40 |
6 |
5000 |
38* |
14 |
43 |
14 |
AF-2 0.1 µg |
406 |
31 |
|
|
2-AA |
|
|
372 |
20 |
|
||||
Salmonella typhimurium TA 1537 |
||||
0 (Water) |
6 |
1 |
19 |
2 |
625 |
|
|
19 |
5 |
1250 |
8 |
3 |
16 |
2 |
2500 |
15 |
3 |
17 |
2 |
5000 |
2* |
2 |
27 |
3 |
9-AA 80 µg |
964 |
18 |
|
|
2-AA |
|
|
90 |
9 |
|
||||
Confirmation Test |
||||
Salmonella typhimurium TA 98 |
||||
0 (Water) |
25 |
2 |
|
|
1000 |
23 |
2 |
|
|
1500 |
26 |
6 |
|
|
2000 |
33 |
3 |
|
|
2500 |
44 |
7 |
|
|
3000 |
42 |
6 |
|
|
3500 |
34* |
10 |
|
|
4000 |
18* |
3 |
|
|
4500 |
11* |
4 |
|
|
5000 |
14* |
4 |
|
|
AF-2 0.1 µg |
365 |
26 |
|
|
|
||||
Salmonella typhimurium TA 1537 |
||||
0 (Water) |
5 |
1 |
|
|
1000 |
7 |
2 |
|
|
1500 |
9 |
2 |
|
|
2000 |
8 |
1 |
|
|
2500 |
13 |
3 |
|
|
3000 |
15 |
5 |
|
|
3500 |
7* |
2 |
|
|
4000 |
4* |
1 |
|
|
4500 |
3* |
1 |
|
|
5000 |
5* |
3 |
|
|
9-AA 80 µg |
933 |
29 |
|
|
* = Toxic effect was observed.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results:
positive without metabolic activation
The test substance was mutagenic in Salmonella typhimurium TA1537 without an exogenous metabolic activation system. - Executive summary:
The study was performed according to OECD TG 471 & TG 472 under GLP conditions.
The test substance induced mutations only in S. typhimurium TA1537 without metabolic activation at 2500 and 3000 µg/plate. The number of the induced revertant colonies/mg was calculated as 3.6/mg. Toxicity was observed at 5000 µg/plate (TA98, TA1537) without S9 mix. In a confirmation test, toxicity was observed at more than 3500 µg/plate (TA98, TA1537) without S9 mix.
Conclusion: The test substance was mutagenic in Salmonella typhimurium TA1537 without an exogenous metabolic activation system.
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