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EC number: 206-058-5 | CAS number: 298-12-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Harlan Laboratories Ltd.,Zelgliweg 1, 4452 Itingen, Switzerland
- Analytical monitoring:
- not required
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The sludge was washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated.
Based on this ratio, an aliquot of washed sludge was suspended in tap water to obtain a concentration equivalent to 3 g dry material per liter. During the holding period of two days prior to use, the sludge was fed daily with 50 mL synthetic sewage feed* per liter and was kept at room temperature under continuous aeration until use. Before use, the dry weight of the activated sludge was measured again in the inoculum used for the test.
* Synthetic sewage feed:
16 g peptone
11 g meat extract
3 g urea
0.7 g NaCl
0.4 g CaCl2 × 2H2O
0.2 g MgSO4 × 7H2O
2.8 g K2HPO4
filled up to a final volume of 1 liter with deionized water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- Not applicable
- Hardness:
- Not applicable
- Test temperature:
- The temperature in the test media, measured in one control, was 20 °C at the start and at the end of the incubation period.
- pH:
- The pH of the activated sludge inoculum was 7.1.
The pH of the stock solution with a Glyoxylic acid 50% concentration of 10 g/L (used to prepare the five test concentrations) was adjusted from pH 1.8 to 7.4 with a 1M sodium hydroxide solution. - Dissolved oxygen:
- The concentration of dissolved oxygen did not drop below 2.5 mg/L during the incubation period. Just before measurement of the respiration rates, the dissolved oxygen concentrations were at least 8.6 mg/L.
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- The inoculum had a sludge concentration of 2.4 g/L dry weight (corresponding to about 1.0 g dry material per liter test medium).
- Details on test conditions:
- The test was performed in 2000-mL glass beakers. The test vessels were labeled with the necessary information to ensure unmistakable identification.
At the start of the test, synthetic sewage feed and activated sludge inoculum (see Sections 3.3 and 3.4.1) were added. The sludge was added in time intervals of 15 minutes (an arbitrary but convenient interval) first to a control, secondly to the test solutions of the reference item, thirdly to the test solutions of the test item, and finally to the second control.
During the incubation period of 3 hours, all test media and the controls were continuously aerated by intense stirring on magnetic stirrers to avoid possible foaming and/or stripping of the test item. - Reference substance (positive control):
- yes
- Remarks:
- Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: Not applicable
- Results with reference substance (positive control):
- The 3-hour EC50 of the reference item 3,5-dichlorophenol (positive control) was calculated to be 10 mg/L (the 95 % confidence limits could not be calculated due to mathematical reasons). The 3-hour EC50 is within the guideline-recommended range of 5–30 mg/L, confirming suitability of the activated sludge used.
- Validity criteria fulfilled:
- yes
- Executive summary:
The inhibitory effect of Glyoxylic Acid 50% on the respiration rate of aerobic wastewater microorganisms of activated sludge was investigated in a 3-hour respiration inhibition test in accordance with OECD TG 209 and GLP. The following nominal concentrations of the test item Glyoxylic Acid 50% were tested: 20, 64, 200, 640 and 2000 mg/L (corresponding to 10, 32, 100, 320 and 1000 mg active ingredient per liter, respectively). In addition, two controls and three different concentrations of the reference item 3,5-dichlorophenol (5, 16 and 50 mg/L) were tested in parallel. The results of these treatments confirmed suitability of the activated sludge and the method used.
Up to and including the highest concentration of 2000 mg/L, the test item Glyoxylic Acid 50% had no significant inhibitory effect (≤10%) on the respiration rate of activated sludge after the incubation period of three hours. Thus, the 3-hour NOEC (EC10) of Glyoxylic acid 50% to activated sludge microorganisms was at least 2000 mg/L (equivalent to 1000 mg active ingredient per liter). This value might even be higher, but concentrations above 2000 mg/L were not tested. The 3-hour EC20, EC50 and EC80 could not be calculated, but were clearly higher than 2000 mg/L.
Reference
Description of key information
Up to and including the highest concentration of 2000 mg/L, the test item Glyoxylic Acid 50% had no significant inhibitory effect (≤10%) on the respiration rate of activated sludge after the incubation period of three hours. Thus, the 3-hour NOEC (EC10) of Glyoxylic Acid 50% to activated sludge microorganisms was at least 2000 mg/L (equivalent to 1000 mg active ingredient per liter). This value might even be higher, but concentrations above 2000 mg/L were not tested. The 3-hour EC20, EC50 and EC80 could not be calculated, but were clearly higher than 2000 mg/L.
Key value for chemical safety assessment
Additional information
The inhibitory effect of Glyoxylic Acid 50% on the respiration rate of aerobic wastewater microorganisms of activated sludge was investigated in a 3-hour respiration inhibition test according to OECD TG 209 and GLP. The following nominal concentrations of the test item Glyoxylic Acid 50% were tested: 20, 64, 200, 640 and 2000 mg/L (corresponding to 10, 32, 100, 320 and 1000 mg active ingredient per liter, respectively). In addition, two controls and three different concentrations of the reference item 3,5-dichlorophenol (5, 16 and 50 mg/L) were tested in parallel. The results of these treatments confirmed suitability of the activated sludge and the method used.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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