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Diss Factsheets
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EC number: 203-614-9 | CAS number: 108-77-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1986-12-09 to 1986-12-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: - Guideline study - GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Version / remarks:
- as at 1983
- Deviations:
- yes
- Remarks:
- : in contrast to the OECD guideline from 1997, only 1000 PCE analyzed
- Principles of method if other than guideline:
- - cells were harvested from bone marrow
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 2,4,6-trichloro-1,3,5-triazine
- EC Number:
- 203-614-9
- EC Name:
- 2,4,6-trichloro-1,3,5-triazine
- Cas Number:
- 108-77-0
- Molecular formula:
- C3Cl3N3
- IUPAC Name:
- trichloro-1,3,5-triazine
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: BOR: NMRI, (SPF Han)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Versuchstierzucht GmbH & Co. KG., D-4799 Borchen
- Age at study initiation: 6 weeks
- Weight at study initiation: 33 - 39 g (males), 26 - 32 g (females)
- Assigned to test groups randomly: yes, under following basis: computer generated random numbers
- Fasting period before study:
- Housing: 1 per cage (Makrolon cage, type II)
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2 °C
- Humidity (%): 50 ± 15 %
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle(s)/solvent(s) used:peanut oil
- Justification for choice of solvent/vehicle: non stated, but test substance decomposes in water
- Concentration of test material in vehicle: 61.9 mg/mL
- Amount of vehicle: 10 mL/kg/bw
- Lot/batch no. : not reported
- Purity: not reported
- Identity of vehicle: Peanut oil (Oleum arachidis DAB 8, Fa. H. Lamotte, D-2800 Bremen) - Details on exposure:
- PREPARATION OF DOSING SOLUTIONS:
- suspension of the test substance in the vehicle was prepared immediately before administration by adding the test substance to the vehicle. The mixture was homogenized using an ultra turrax homogenizer (Janke u. Kunkel, D-7813 Staufen, Germany)
- Duration of treatment / exposure:
- - single dosage via gavage
- Frequency of treatment:
- - single dosage via gavage
- Post exposure period:
- - 24, 48, and 72 h post administration 6 animals of each sex were sacrificed for examination
Doses / concentrations
- Remarks:
- Doses / Concentrations:
619 mg/kg bw
Basis:
other: via gavage
- No. of animals per sex per dose:
- 18
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- cyclophosphamide
- Justification for choice of positive control(s): none stated
- Route of administration: oral via gavage
- Dose: 51.1 mg/kg bw, dissolved in 0.9% saline solution
Examinations
- Tissues and cell types examined:
- bone marrow cells from the femurs
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION:
Obvious clinical symptoms of intoxication and deaths were observed at 1000 mg/kg bw in an orientating study
TREATMENT AND SAMPLING TIMES ( in addition to information in specific fields):
single dosage via gavage, sampling at 24, 48, and 72 h post administration (6 animals of each sex)
DETAILS OF SLIDE PREPARATION:
- at least two slides per animals were prepared
- animals were sacrificed by CO2 overdose
- both femurs removed and bone marrow cells flushed into labelled centrifuge tubes with 1.5 ml fetal calf serum (Art. No. 210463, Boehringer Mannheim, D-6800 Mannheim 1)
- centrifugation of tubes: 1000 rpm, 5 min (Labofuge 6000, Heraeus-Christ GmbH, D3360 Osterode am Harz)
- supernatant discarded, bone marrow cells suspended upon a thin layer of serum
- the marrow serum suspension was smeared on a slide and dried over night (slide identification: project no., animal no., species, sex, material, date of preparation)
- two slides were stained using the panoptic stain method (Pappenheim et. al, Das Knochenmark, p. 12, ed. Queisser W., Georg Thieme Verlag, Stuttgart, 1978)
METHOD OF ANALYSIS:
- 1000 PCE were scored for the incidence of micronuclei under the microscope (magnification 1000 x, C. Zeiss, D-7082 Oberkochem, Germany) (one slide used per animal, the second as back up)
OTHER:
- The ration of polychromatic to normochromatic erythrocytes was calculated based on 1000 erythrocytes as a measure of the toxic efficacy of the test material - Evaluation criteria:
- - If a test material produced no statistical significant and reproducible positive response at any one of the test points compared to the negative control group, it was considered non mutagenic in this system (significance level: 5%)
- Statistics:
- - The frequencies of micronuclei of the test group and the positive control group were compared with those of the negative control group at each sampling time.
- A POISSON test was applied. Estimation and test for each treatment group and each sex by means of a VAX 750 computer
Results and discussion
Test resultsopen allclose all
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- two animals died, one at day 1, one at day 3 post administration
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Sex:
- female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- two animals died, both on day 2 post administration
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Based on the results of the present mammalian erythrocyte micronucleus test cyanuric chloride can be regarded as non mutagenic under the test conditions.
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