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EC number: 217-168-8 | CAS number: 1761-71-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-05-14 - 1990-05-17
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test procedure according to national standard with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: German Industrial Standard DIN 38412, Part 9
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- - stock solution: 400 mg test substance / L bidest. water 80 mg solvent (Tween 80) / L bidest. water
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80 - Details on test organisms:
- TEST ORGANISM
- Common name: Scenedesmus subspicatus CHODAT
- Source (laboratory, culture collection): Supplier: Sammlung für Algenkulturen der Universität Goettingen
- medium: according to DIN 38 412, Part 9
- cultivation flasks/volume: 250 ml-Erlenmeyer flasks, 100 ml liquid volume
- reinoculation: once per week - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- pH:
- pH range at start: - 8.2 (control [w/o Tween 80], control [w Tween 80]) - 10.9 (200 mg/L)
pH range after 72 h: - 9.0 (control [w Tween 80]) - 9.6 (141.42 mg/L) - Nominal and measured concentrations:
- - test concentrations (nominal): 35.36, 50, 70.71, 100, 141.42, 200 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: test tubes
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, 10-mL liquid volume
- Aeration: shaken twice a day to hold cells in suspension
- Initial cells density: 10000 cells/mL
- replicates: per concentration, control (w/o Tween 80), control (w Tween 80): 4; additional non-inoculated samples were prepared to check intrinsic fluorescence of the test substance
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: artificial light, approx. 120 µE/(m2*s)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter: 0, 24, 48 and 72 h (fluorescence: in-vivo chlorophyll-a fluorescence at 685 nm as a size for the biomass (pulsed excitation with light flashes having a wavelength of 435 nm)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 1.4
- Justification for using less concentrations than requested by guideline: not applicable - Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 141.42 - <= 200 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: based on not pH-adjusted test solutions
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: based on not pH-adjusted test solutions
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- >= 141.42 - <= 200 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: based on not pH-adjusted test solutions
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- < 35.36 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: based on not pH-adjusted test solutions
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 141.42 - <= 200 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: based on not pH-adjusted test solutions
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- >= 141.42 - <= 200 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: based on not pH-adjusted test solutions
- Conclusions:
- Based on the nominal initial concentrations of the test item, the 72 -hour EC50 for green algae biomass (non pH adjusted) was between 141,42 and 200 mg/L.
- Executive summary:
The toxicity of the test item was determined according to German Industrial Standard DIN 38412, Part 9.
The study was conducted under static conditions over a period of 72 hours with a negative control a solvent control and six nominal test concentrations: 35.36, 50, 70.71, 100, 141.42, 200 mg/L. TWEEN 80 was used as a solving agent.These chosen concentrations were based on the results from a range finding toxicity with the test item. The solutions were not pH adjusted.
16000 mg/L green algae Scenedesmus subspicatus were exposed with the test item in 10 mL test medium in glass tubes at20 ±1°C under permanent light. The tubes were stirred twice a day against sedimentation of algae. pH values were determined at initiation and termination of the study.
Based on the nominal initial concentrations of the test item, the 72 -hour EC50 for green algae biomass (non pH adjusted) was between 141,42 and 200 mg/L.
Reference
Biomass, growth rate and inhibition data
Test concentration |
Biomass |
Growth rate |
Inhibition |
Inhibition |
nominal (mg/L) |
B |
µ |
%, biomass |
%, growth rate |
solvent control |
1.88 |
1.17 |
|
|
0.00 |
1.75 |
1.17 |
7.10 |
-0.29 |
35.36 |
1.55 |
1.08 |
17.53 |
7.66 |
50.00 |
1.59 |
1.10 |
15.67 |
5.60 |
70.71 |
1.61 |
1.12 |
14.65 |
4.27 |
100.00 |
1.51 |
1.05 |
19.88 |
10.33 |
141.42 |
1.26 |
0.99 |
33.07 |
14.91 |
200.00 |
|
-0.40 |
100.00 |
100.00 |
pH values at 0 h and 72 h
Test concentration (mg/L) |
pH |
|
0 hour |
72 hour |
|
0 (control) |
8.2 |
9.2 |
0 (solvent control) |
8.2 |
9.0 |
35.36 |
8.4 |
9.4 |
50.00 |
8.5 |
9.5 |
70.71 |
8.6 |
9.6 |
100.00 |
8.8 |
9.4 |
141.42 |
9.3 |
9.6 |
200.00 |
10.9 |
9.2 |
Description of key information
The EC50 / EC10 for aquatic algae and cyanobacteria was based on the 72 hour growth rate inhibition from the key study on Desmodesmus subspicatus (BASF, 1990).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 141.42 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
The 72-hour ECr50 of 141.42 mg/L (ECr10 of ~100mg/L) for non-pH adjusted solution for Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) was the representative value for the short-term toxicity of 4,4'-methylenebis(cyclohexylamine) to aquatic algae and cynaobacteria. This value represented one of the three short-term aquatic toxicity values for each of three trophic levels (fish, aquatic invertebrates, and aquatic algae and plants). The lowest of the three short-term aquatic toxicity values from each of the three trophic levels was used as the point of departure for the derivation of the PNECaquatic (freshwater).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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