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EC number: 205-745-7 | CAS number: 149-73-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1966
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Study performed in 1966, probably no GLP, reasonably documented (2-page summary report). Only male animals investigated (6 per dose). Necropsy and histopathological evaluation, but shortened observation time (1-7 days) in lowest dose group. Submitted to US Office of Toxic Substances under TSCA, 1982.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 966
- Report date:
- 1966
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Four-hour vapour inhalation, 5 dose groups of 6 male rats each. Time of death and symptoms recorded, weight development described, sacrifice and macro-/histopathology after 14 days (except lowest group).
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Trimethyl orthoformate
- EC Number:
- 205-745-7
- EC Name:
- Trimethyl orthoformate
- Cas Number:
- 149-73-5
- Molecular formula:
- C4H10O3
- IUPAC Name:
- trimethoxymethane
- Details on test material:
- - Name of test material (as cited in study report): Trimetyl orthoformate TMOF
- Substance type: pure active substance
- Physical state: liquid
- Analytical purity: 98%
- Vapour pressure: 50 mm Hg (6.67 kPa) at 30°C
- Boiling point: 100.6°C
- Impurities (identity and concentrations): no data
- Used as received
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: ChR-CD
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 244-297 g
- Housing, diet, water, temperature, humidity etc.: no data
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: chamber containing 6 animals
- Exposure chamber volume: 16 L
- Method of holding animals in test chamber: not specified
- Source and rate, conditioning of air: no data
- System of generating vapour: Metering of test substance by syringe drive into T-tube heated to 120-140°C; stream of air carrying the vapour into the test chamber
- Method of particle size determination: not applicable to vapor (maximum concentration approximately 1/7 of saturation conc.)
- Treatment of exhaust air: not specified
- Temperature, humidity, pressure in air chamber: no data
TEST ATMOSPHERE
- Brief description of analytical method used: Hourly sampling of chamber atmosphere and determination of test substance by gas chromatography
- Samples taken from breathing zone: yes (assuming a homogeneous chamber atmosphere)
VEHICLE
- none
TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: not applicable to vapor (approximately 1/7 of saturation concentration reached in chamber) - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- gas chromatography
- Duration of exposure:
- 4 h
- Concentrations:
- 47.8, 44.0, 38.0, 36.2, 16.5 mg/L
- No. of animals per sex per dose:
- 6 males
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days (except for lowest dose 16.5 mg/L: 2 rats each sacrificed at 1, 2, and 7 days post-exposure)
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, histopathology - Statistics:
- Litchfield JT & Wilcoxon F (1949) J. Pharmacol. Exp. Therap. 96: 99-113
Results and discussion
Effect levels
- Key result
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- > 40 mg/L air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- Ranging from 6/6 at top dose to 0/6 at lowest dose. Death times: from 3 h 50 min (during the exposure) to 2 days post-exposure.
Sacrifice of survivors after 14 days (except lowest dose group, which had interim sacrifices of 2 animals each on days 1, 2, and 7). - Clinical signs:
- other: During exposure: lacrimation, salivation, hyperpnea, dyspnea, hyperemia at all levels. Severe moist rales at lethal levels only. Unresponsiveness to slight responsiveness at lethal levels, mild responsiveness at non-lethal level. Post-exposure: Unresponsi
- Body weight:
- Normal weight gain after initial loss at lethal levels. Normal weight gain throughout at non-lethal level.
- Gross pathology:
- No gross pathalogical effects attributable to the test substance.
- Other findings:
- - Histopathology: pulmonary and hepatic congestions; other heaptic effects (not specified). Effects reversible: not found in survivors after 14 days, nor in interim sacrifices of the lowest dose group.
- Potential target organs: Lungs and liver; no findings in brain, spleen, bone marrow, testis, thymus, stomach, intestine, and skin.
- Other observations: Clinical signs and histologic findings indicate that trimethyl orthoformate is a respiratory irritant.
Any other information on results incl. tables
Mortaliy
Concentration (mg/L | Mortality ratio |
47.8 | 6 / 6 |
44.0 | 5 / 6 |
38.0 | 2 / 6 |
36.2 | 1 / 6 |
16.2 | 0 / 6 |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The substance does not meet GHS classification criteria for acute inhalation toxicity.
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