Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 206-743-9 | CAS number: 372-09-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412, part L15
- GLP compliance:
- no
Test material
- Reference substance name:
- Cyanoacetic acid
- EC Number:
- 206-743-9
- EC Name:
- Cyanoacetic acid
- Cas Number:
- 372-09-8
- Molecular formula:
- C3H3NO2
- IUPAC Name:
- cyanoacetic acid
- Details on test material:
- - Name of test material (as cited in study report): Cyanoacetic acid
- Analytical purity: 99.9 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solution was prepared by adding the test item to the test media without any pre-treatment, subsequently the fish were placed into the aquaria.
Test organisms
- Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, 2354 Hohenweststedt, Germany
- Length at study initiation (length definition, mean, range and SD): 7.6 cm (range 7.0 - 8.6 cm)
- Weight at study initiation (mean and range, SD): 4.0 g (range 2.7 - 6.1 g)
ACCLIMATION
- Acclimation period: 3 days
- Acclimation conditions (same as test or not): same
- Type and amount of food: Growing feed FB 50, Ssniff Spezialdiaeten GmbH, Soest, Germany; ad libitum
- Feeding frequency: 1 day before and during exposure
- Health during acclimation (any mortality observed): no mortality observed
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 2.5 mmol/l
- Test temperature:
- 20 - 21 °C
- pH:
- 8.0
- Dissolved oxygen:
- 7.1 - 8.8 mg/l
- Nominal and measured concentrations:
- Nominal test concentration (mg/l):
10.0,
21.5,
46.4,
100,
215,
control,
pH control (215 mg/l) - Details on test conditions:
- TEST SYSTEM
- Test vessel: All glass aquaria (30 x 22 x 24 cm)
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 4 g fish/l
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982.
- Ca/mg ratio: 4 : 1
OTHER TEST CONDITIONS
- Adjustment of pH: at a concentration of 215 mg/l
- Photoperiod: 16 : 8 hours day-night regime
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Results used to determine the conditions for the definitive study: LC 50 (96h) 10 > 100 mg/l - Reference substance (positive control):
- yes
- Remarks:
- Chloroacetamide
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 67.82 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: ; (geom. mean)
- Details on results:
- The test substance causes a change in the pH value of the test system. The results refer to an unneutralised sample. After neutralisation of the test solution at a test concentration level of 215 mg/l, cyanoacetic acid was observed to have a significant reduction in the level of toxicity.
- Results with reference substance (positive control):
- 48 h-LC50 of the positive control chloroacetamide: about 31 mg/l (corresponds to normal sensitivity).
Any other information on results incl. tables
- Sublethal observations / clinical signs:
- Nominal test / Cumulative mortality (%) after x hours /
concentration / 1 / 4 / 24 / 48 / 72 / 96 /
(mg/l)
Control / 0 / 0 / 0 / 0 / 0 / 0 /
1.0.0 / 0 / 0 / 0 / 0 / 0 / 0 /
21.5 / 0 / 0 / 0 / 0 / 0 / 0 /
46.4 / 0 / 0 / 0 / 0 / 0 / 0 /
100.0 / 60 / 100 / 100 / 100 / 100 / 100 /
215.0 / 100 / 100 / 100 / 100 / 100 / 100 /
Control / 0 / 0 / 0 / 0 / 0 / 0 /
215.0** / 0 / 0 / 0 / 0 / 0 / 100 /
(** = neutralized)
Symptoms of intoxication:
Nominal test / Symptoms of intoxication after x hours
concentration / 1 / 4 / 24 / 48 / 72 / 96 /
(mg/l)
Control / - / - / - / - / - / - /
10.0 / - / - / - / - / - / - /
21.5 / - / - / - / - / - / - /
46.4 / - / - / - / - / - / - /
100.0 / AN / + / + / + / + / + /
215.0 / + / + / + / + / + / + /
Control / - / - / - / - / - / - /
215.0 / - / - / - / - / - / + /
(** = neutralized)
Definitions of Symptoms:
-: No symptoms
A: Apathy
N: Narcotic-like state
+: All fish dead
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.