Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-706-1 | CAS number: 124-63-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.12 (Mutagenicity - In Vivo Mammalian Erythrocyte Micronucleus Test)
- Version / remarks:
- Cited as Directive 2000/32/EC, B.12
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Methanesulphonyl chloride
- EC Number:
- 204-706-1
- EC Name:
- Methanesulphonyl chloride
- Cas Number:
- 124-63-0
- Molecular formula:
- CH3ClO2S
- IUPAC Name:
- methanesulfonyl chloride
- Details on test material:
- Test article name : Methane sulfonyl chloride
Cas n°: 124-63-0
Origin: Atofina NA, Riverview, USA
Batch: 1491F10CD1
Purity: 99.9 %
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- Swiss
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST SYSTEM
Animals:
Strain: Swiss Ico: OF1 (IOPS Caw).
Breeder: Charles River Laboratories France, l'Arbresle, France.
Age: 6 weeks old on the day of treatment
Acclimation: at least 5 days
ENVIRONMENTAL CONDITIONS:
- temperature: 22 ± 2°C,
- relative humidity: 30 to 70%,
- light/dark cycle: 12 h/12 h (07:00 - 19:00),
- ventilation: at least 12 cycles/hour of filtered non-recycled fresh air.
Housing:
The animals were housed by groups in polycarbonate cages.
Food and water:
Food: A04 C pelleted maintenance diet (SAFE, Villemoisson-sur-Orge, France), ad libitum.
Water: drinking water filtered by a FG Millipore membrane (0.22 micron), ad libitum.
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- - Vehicle(s)/solvent(s) used: paraffin oil
- Justification for choice of solvent/vehicle: good solubility
- Concentration of test material in vehicle: the test item was supplied as preparations at 200.25 mg/mL and 20.03 mg/mL in
paraffin oil. The latter was used directly for treatment or diluted in the vehicle immediately before use, depending on the target dose-level..
- Amount of vehicle (if gavage or dermal): 10 ml/kg - Details on exposure:
- EXPERIMENTAL DESIGN
Range-finding toxicity assays:
In order to select the top dose-level for the cytogenetic study, 200.3, 50 or 30 mg/kg/day were administered twice, to three males and three females. The interval between each administration was 24 hours. Clinical signs and any mortality were recorded for a period of 48 hours.
Micronucleus assay:
Administration:
Three groups of five male and five female mice were given intraperitoneal administrations (10 ml/kg) of Methane Sulfonyl Chloride at dose-levels of 0 (paraffin oil), 7.5,15 and 30 mg/kg/day, over a 2-day period. One group of five males and five females received the positive control test item (cyclophosphamide) once by oral route at the dose-level of 50 mg/kg. - Duration of treatment / exposure:
- 2 administrations at 24 hours interval
- Frequency of treatment:
- daily
- Post exposure period:
- 24 h
Doses / concentrations
- Remarks:
- Doses / Concentrations:
7.5, 15 and 30 mg/kg/day
Basis:
- No. of animals per sex per dose:
- 5 or 8
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- The positive control was cyclophosphamide (CPA) dissolved in distilled water at a concentration of 5 mg/mL.
Examinations
- Tissues and cell types examined:
- Bone marrow
- Details of tissue and slide preparation:
- Preparation of the bone marrow smears:
The animals of the treated and vehicle control groups were killed 24 hours after the last treatment and the animals of the positive control group were killed 24 hours after the single treatment. The femurs of the animals were removed and the bone marrow was flushed out using fetal calf serum. After centrifugation, the supernatant was removed and the cells in the sediment were resuspended by shaking. A drop of this cell suspension was placed and spread on a slide. The slides were air-dried and stained with Giemsa. The slides were coded so that the scorer is unaware of the treatment group of the slide under evaluation ("blind" scoring).
Microscopic examination of the slides:
For each animal, the number of the micronucleated polychromatic erythrocytes (MPE) was counted in 2000 polychromatic erythrocytes; the polychromatic (PE) and normochromatic (NE) erythrocyte ratio was established by scoring a total of 1000 erythrocytes (PE + NE). - Evaluation criteria:
- For a result to be considered positive, a statistically significant increase in the frequency of MPE must be demonstrated when compared to the concurrent vehicle control group. Reference to historical data, or other considerations of biological relevance was also taken into account in the evaluation of data obtained.
- Statistics:
- When there was no significant within-group heterogeneity, using the heterogeneity chi-square test value (Lovell et al., 1989), the frequencies of MPE in each treated group was compared with those in the concurrent vehicle control groups by using a 2 x 2 contingency table to determine the X2 value (Lovell et al., 1989).
When there was significant within-group heterogeneity, then that group was compared with the control group using a non-parametric analysis, the Mann-Whitney test (Schwartz, 1969).
The student "t" test was used for the PE/NE ratio comparison.
Probability values of p <= 0.05 was considered as significant.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- Hypoactivity and piloerection were noted in all treated animals at 30 mg/kg/day
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- PRELIMINARY TOXICITY TEST
At 200.3 mg/kg/day, 3/3 males and 2/3 females dead in the 24 hours following the first treatment.
At 50 mg/kg/day, hypoactivity and piloerection were noted in all treated animals. At the end of the observation period no mortality was noted. However, at the time of sacrifice of the animals 24 hours later, 3/6 treated animals were found dead showing the high toxicity of this
dose.
At 30 mg/kg/day, hypoactivity and piloerection were noted in all treated animals but no mortality was observed.
CYTOGENETIC TEST
No clinical signs and no mortality were observed in the animals of both sexes given 7.5 or 15 mg/kg/day. Hypoactivity and piloerection were noted in all treated animals at 30 mg/kg/day.
For both males and females, the mean values of MPE as well as the PE/NE ratio in the groups treated with the test item, were equivalent to those of the vehicle group.
Cyclophosphamide induced a highly significant increase (p < 0.001) in the frequency of MPE, indicating the sensitivity of the test system under our experimental conditions. The study was therefore considered valid.
Any other information on results incl. tables
Results of the cytogenetic test: data summary
Group |
Doses |
MPE/1000PE |
PE/NE ratio |
Time of sacrifice after the last administration |
||
(mg/kg/day) |
mean |
(sd) |
mean |
(sd) |
24 h |
|
Males |
||||||
Vehicle |
- |
1.7 |
(0.6) |
0.3 |
(0.1) |
|
Test item |
7.5 |
1.4 |
(1.0) |
0.4 |
(0.1) |
|
15 |
1.1 |
(0.7) |
0.5 |
(0.2) |
||
30 |
0.9 |
(0.4) |
0.3 |
(0.1) |
||
Cyclophosphamide |
50 mg/kg |
18.6 |
(7.1)*** |
0.5 |
(0.1) |
|
Females |
24 h |
|||||
Vehicle |
- |
0.9 |
(0.5) |
0.6 |
(0.2) |
|
Test item |
7.5 |
0.9 |
(1.1) |
0.8 |
(0.2) |
|
15 |
1.5 |
(0.6) |
0.4 |
(0.1) |
||
30 |
0.6 |
(0.8) |
0.5 |
(0.1) |
||
Cyclophosphamide |
50 mg/kg |
17.1 |
(5.4)*** |
0.6 |
(0.1) |
MPE: Micronucleated Polychromatic Erythrocytes
PE: Polychromatic Erythrocytes
NE: Normochromatic Erythrocytes
sd: Standard Deviation
*** p < 0.001
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Methane Sulfonyl Chloride (purity: 99.9%) did not induce damage to the chromosomes or the mitotic apparatus of mice bone marrow cells after two intraperitoneal administrations, with a 24-hour interval, at the dose-levels of 7.5, 15 and 30 mg/kg/day. - Executive summary:
The potential of methanesulfonyl chloride (purity 99.9%) to induce structural or numerical damage in bone marrow cells of mice was evaluated a study performed according to the OECD guideline #474 and GLP.
Three groups of five male and five female mice were given intraperitoneal administrations (10 ml/kg) of methanesulfonyl chloride at dose-levels of 0 (paraffin oil), 7.5,15 and 30 mg/kg/day, over a 2-day period. One group of five males and five females received the positive control test item (cyclophosphamide) once by oral route at the dose-level of 50 mg/kg.
Animal were sacrificed 24 hours after the last administration, and bone marrow smears examined for the presence of micronuclei in 2000 polychromatic erythrocytes per mouse and for the ratio PCE/NCE.
For both males and females, the mean values of MPE as well as the PE/NE ratio in the groups treated with the test item, were equivalent to those of the vehicle group. Methanesulfonyl chloride was concluded to be negative in the mouse micronucleus assay.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.