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EC number: 203-013-1 | CAS number: 102-20-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish, juvenile growth test
- Type of information:
- experimental study
- Remarks:
- Experimental data of read across substances
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data for the target chemical is summarized based on the read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- WoE report is based on two long term toxicity to fish studies as-
WoE 2 and WoE 3. - GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- WoE 2 - Concentrations: (nominal values) control, vehicle control, concentration 1: 0.020 mg/L, concentration 2: 0.058 mg/L, concentration 3: 0.17 mg/L, concentration 4: 0.48 mg/L, concentration 5: 1.4 mg/L (geometric ratio: 2.9)
- Vehicle:
- yes
- Remarks:
- WoE 2- N,N-Dimethylformamide, HCO-60
- Details on test solutions:
WoE 2- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): N,N-Dimethylformamide, HCO-60
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): constant concentration, N,N-Dimethylformamide: ca. 100 microL/L, HCO-60: ca. 2.0 mg/L- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- WoE 2- TEST ORGANISM
- Common name: Japanese rice fish
POST-HATCH FEEDING
- Type/source of feed: BSN* (*: brine shrimp hatching larva just after hatching) for larval and juvenile fish)
- Amount given: full feeding - Frequency of feeding: 1- 2 times per day
WoE 3- TEST ORGANISM
- Common name: Japanese rice fish - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 42 d
- Remarks on exposure duration:
- WoE 2- exposed from fertilized egg, until after 30 days from the day when hatching rate became more than 70 % in control
WoE 3- 28 days - Test temperature:
- WoE 2- egg and embryo stages: 24 ±1 °C larval and juvenile fish stages: 23 ± 2 °C
- Dissolved oxygen:
- WoE 2- maintained more than 60% of the saturated concentration (not aeration)
- Nominal and measured concentrations:
- WoE 2- (nominal values) control, vehicle control, concentration 1: 0.020 mg/L, concentration 2: 0.058 mg/L, concentration 3: 0.17 mg/L, concentration 4: 0.48 mg/L, concentration 5: 1.4 mg/L (geometric ratio: 2.9)
- Details on test conditions:
- WoE 2- TEST SYSTEM
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): test solution supply volume: 48 L/vessel/day, liquid volume in test vessel: 2.5 L (renewal rate: 19 times/day)
- No. of fertilized eggs/embryos per vessel: 60/concentration (20/vessel)
- No. of vessels per concentration (replicates): 3
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light per 8 hours darkness
- Light intensity: less than 1000 lux
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 0.154 - < 0.92 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: NOEC value of test chemical was determined to be > 0.154 to < 0.92 mg/l.
- Key result
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 0.435 - < 1.92 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: LOEC value of test chemical was determined to be > 0.435 to < 1.92 mg/l.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 28 days NOEC value of test chemical was determined to be > 0.154 to < 0.92 mg/l and LOEC value was determined to be > 0.435 to < 1.92 mg/l.
- Executive summary:
Data available of the structurally similar read across chemicals have been reviewed to determine the effect of the test chemical on fishes. The studies are as mentioned below:
A first study of long-term toxicity to fish was carried out according to OECD 210 guideline. Oryzias latipes (Japanese rice fish) was taken as test organism. The duration of test was 42 days, exposed from fertilized egg, until after 30 days from the day when hatching rate became more than 70 % in control. Post-hatch feeding includes BSN type of food (*: brine shrimp hatching larva just after hatching for larval and juvenile fish). Full feeding was given to test fishes, frequency of feeding was 1- 2 times per day. N,N-Dimethylformamide, HCO-60 was taken as vehicle, N,N-Dimethylformamide was taken as ca. 100 microL/L, HCO-60 was taken as ca. 2.0 mg/L. Test concentrations were taken as 0.020 mg/L, 0.058 mg/L, 0.17 mg/L, 0.48 mg/L, 1.4 mg/L in geometric ratio of 2.9. The renewal rate of the test solution was 19 times/day, 48 L/vessel/day. The liquid volume in test vessel was 2.5 L. Number of fertilized eggs/embryos per vessel was 60 per concentration, 20 per vessel. The number of vessels per concentration replicates was taken as three. Adjustment of pH was not adjusted. Photoperiod was given as 16 hours of light per 8 hours darkness. Light intensity was given as less than 1000 lux. Test temperature at egg and embryo stages was 24 ±1 °C and at larval -juvenile fish stages was 23 ± 2 °C. Dissolved oxygen was maintained at more than 60% of the saturated concentration. Analytical monitoring was done by HPLC. On the basis of survival rate effect of test chemical on test organisms Oryzias latipes (Japanese rice fish) the 42 days NOEC and LOEC value of test chemical was determined to be 0.154 mg/l and 0.435 mg/l respectively. Based of the value of NOEC the test chemical was classified as Chronic category 2 as per CLP criteria.
The second study of long-term toxicity to fish was carried out. Oryzias latipes (Japanese rice fish) was taken as test organism. The duration of test was 28 days. On the basis of growth effect of test chemical on test organisms Oryzias latipes (Japanese rice fish) the 28 days NOEC and LOEC value of test chemical was determined to be 0.92 mg/l and 1.92 mg/l respectively. Based of the value of NOEC the test chemical was classified as 'Chronic category 2' as per CLP criteria.
On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 28 days NOEC value of test chemical was determined to be > 0.154 to < 0.92 mg/l and LOEC value was determined to be > 0.435 to < 1.92 mg/l. Based of the value of NOEC the test chemical was classified as 'Chronic category 2' as per CLP criteria.
Reference
Description of key information
On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 28 days NOEC value of test chemical was determined to be > 0.154 to < 0.92 mg/l and LOEC value was determined to be > 0.435 to < 1.92 mg/l.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- NOEC
- Effect concentration:
- > 0.154 - < 0.92 mg/L
Additional information
Data available of the structurally similar read across chemicals have been reviewed to determine the effect of the test chemical on fishes. The studies are as mentioned below:
The first study of long-term toxicity to fish was carried out according to OECD 210 guideline. Oryzias latipes (Japanese rice fish) was taken as test organism. The duration of test was 42 days, exposed from fertilized egg, until after 30 days from the day when hatching rate became more than 70 % in control. Post-hatch feeding includes BSN type of food (*: brine shrimp hatching larva just after hatching for larval and juvenile fish). Full feeding was given to test fishes, frequency of feeding was 1- 2 times per day. N,N-Dimethylformamide, HCO-60 was taken as vehicle, N,N-Dimethylformamide was taken as ca. 100 microL/L, HCO-60 was taken as ca. 2.0 mg/L. Test concentrations were taken as 0.020 mg/L, 0.058 mg/L, 0.17 mg/L, 0.48 mg/L, 1.4 mg/L in geometric ratio of 2.9. The renewal rate of the test solution was 19 times/day, 48 L/vessel/day. The liquid volume in test vessel was 2.5 L. Number of fertilized eggs/embryos per vessel was 60 per concentration, 20 per vessel. The number of vessels per concentration replicates was taken as three. Adjustment of pH was not adjusted. Photoperiod was given as 16 hours of light per 8 hours darkness. Light intensity was given as less than 1000 lux. Test temperature at egg and embryo stages was 24 ±1 °C and at larval -juvenile fish stages was 23 ± 2 °C. Dissolved oxygen was maintained at more than 60% of the saturated concentration. Analytical monitoring was done by HPLC. On the basis of survival rate effect of test chemical on test organisms Oryzias latipes (Japanese rice fish) the 42 days NOEC and LOEC value of test chemical was determined to be 0.154 mg/l and 0.435 mg/l respectively. Based of the value of NOEC the test chemical was classified as Chronic category 2 as per CLP criteria.
The second study of long-term toxicity to fish was carried out. Oryzias latipes (Japanese rice fish) was taken as test organism. The duration of test was 28 days. On the basis of growth effect of test chemical on test organisms Oryzias latipes (Japanese rice fish) the 28 days NOEC and LOEC value of test chemical was determined to be 0.92 mg/l and 1.92 mg/l respectively. Based of the value of NOEC the test chemical was classified as 'Chronic category 2' as per CLP criteria.
On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 28 days NOEC value of test chemical was determined to be > 0.154 to < 0.92 mg/l and LOEC value was determined to be > 0.435 to < 1.92 mg/l. Based of the value of NOEC the test chemical was classified as 'Chronic category 2' as per CLP criteria.
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