Ibuprofen

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

number of replciates lower than recommended in guideline, less soil per replicate

Data source

Reference

Reference Type:

publication

Title:

Acute toxicological effects on the earthworm Eisenia fetida of 18 common pharmaceuticals in artificial soil

Author:

M Rosa Pino, Jonatan Val, Ana M Mainar, Estefanía Zuriaga, Cecilia Español, Elisa Langa

Year:

2015

Bibliographic source:

Science of the Total Environment 518–519 (2015) 225–237

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Version / remarks:

1984

Deviations:

yes

Remarks:

3 replicates instead of 4; 500 g soil instead of 750 g soil per replicate

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ibuprofen

EC Number:

239-784-6

EC Name:

Ibuprofen

Cas Number:

15687-27-1

Molecular formula:

C13H18O2

IUPAC Name:

2-(4-isobutylphenyl)propanoic acid

Specific details on test material used for the study:

Purity: 100%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: separate assay without earthworms: 0.0100 g/5 g sampled after 14 d

Test substrate

Vehicle:

no

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Remarks:

standardized OECD soil substrate: industrial fine sand, sphagnum peat, and kaolin clay in a 7:1:2 ratio, water added at amount of 35% of soil dw

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

20±2 °C

Moisture:

80-85% air humidity

Details on test conditions:

TEST SYSTEM
- Test container (material, size): 1 L polypropylene rectangular jars with lid
- Amount of soil or substrate: 500 g ww
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 3
- No. of replicates per control: 3

OTHER TEST CONDITIONS
- Photoperiod: constant light
- Light intensity: 400-800 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- mortality after 7 and 14 d

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 0–10–200–1000 mg/kg soil dw
- Results used to determine the conditions for the definitive study: not reported

Nominal and measured concentrations:

nominal: 0, 15, 30, 50, 60, 80, 100, 150 mg/kg dw soil (stability verified in separate assay)

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Results and discussion

Effect concentrations

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

64.8 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL: 58.49–71.78 mg/kg soil dw, P < 0.0001, R² of the regression = 0.961

Details on results:

- Mortality in negative control at end of exposure period: < 10%

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: 100% mortality at 24 mg/kg soil dw
- Other:

Reported statistics and error estimates:

log Probit analysis