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EC number: 226-641-8 | CAS number: 5444-75-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 Jul - 01 Aug 2000
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Appropriate weight of the test substance were added directly to the test beakers. Dechlorinated tap water (284 mL) was added and the mixtures were treated with ultrasound for 10 min. Then synthetic sewage and inoculum were added. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source of inoculum: Activated sludge was obtained on the day before test start from Oakley Sewage Treatment Works, UK, which treats predominantly domestic waste.
- Laboratory culture: The sample was maintained under aerobic conditions until required.
- Preparation of inoculum for exposure: Aliquots (25 mL) of the sludge were filtered through Whatman GFC filter papers. Synthetic sewage (50 mL/L) was added and the mixture aerated overnight.
- Initial biomass concentration: 4 g/L mixed liquor suspended solids - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 19.3 - 20.3 at test start
18.6 - 19.2 at test end - pH:
- 7.5 - 7.7 at test start
7.9 - 8.1 at test end - Dissolved oxygen:
- 5.0 - 6.5 mg/L at test start
2.5 mg/L at test end - Nominal and measured concentrations:
- Nominal: 0, 1, 10 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Respiration rate was measured in BOD bottles of 270 mL capacity.
- Aeration: The prepared mixtures were aerated for 3 h.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water
- Hardness: 200 - 250 mg/L as CaCO3
- Intervals of water quality measurement: Temperature and pH of the samples were measured at test start and test end.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Respiration rate was measured after 3 h of incubation.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Control: The specific respiration rate of the control at test end (27.8 mg O2/g*h) was 104% of the rate at test start (26.8 mg O2/g*h).
- Test samples: Respiration rate was 27.3, 28.3 and 30.0 mg O2/g*h at 1, 10 and 100 mg test item/L, respectively.
- Effect concentrations exceeding solubility of substance in test medium: Yes - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50 (3h) = 11.5 mg/L (95% CL = 9.2 - 14.8 mg/L)
Reference
Description of key information
No adverse effects on aquatic micro-organisms were observed at the highest test concentration (100 mg/L)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
The toxicity of 2-ethylhexyl benzoate to micro-organisms was investigated in a respiration inhibition test according to OECD guideline 209 and GLP (Barnes, 2000). The substance had no inhibitory effect on the respiration rate of activated sludge following three hours of exposure at 1, 10 and 100 mg/L. Therefore, the three-hour EC50 could not be calculated, but is thus considered to be greater than 100 mg/L, the highest level tested.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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